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First grow ::UPDATE::
    #10465557 -

Just inoculated 16 jars ( was trying to get 20 but first few jars got a lilllll to much solution) hopefully this goes out well!!

I did the RR videos to a T. Hell i lysol'd the room down so much then took a shower and even wore a tyvek suit with all loose ends taped down with gloves and face mask

Only time will tell now:cool:

UPDATE:

Ok its only been 3 days since the inoculation and all seems to be going good, the tex jars have no signs of colonization but its still early, the B+ on the other hand ( which i used 10cc for 6jars compared to 10cc for 10 jars on the tex ) i THINK i am starting to see growth, i noticed a few mm wide circles forming i believe(white of course) under the inoc hole soooo we'll see what happens.

Just gotta be happy for no contams so far..:cool:

UPDATE2:
WOOOOOO, got home from today and the Texans finally showed growth in most the jars, the b+ have FAR taking off and got some GOOOD rizo myc. in them, i would up pictures but only cam i got is a shitty webcam so not even worth it, oh btw its been 5 days since inoculation.

Edited by Fangaz420 (06/12/09 08:30 PM)

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Re: First grow [Re: Fangaz420]
    #10465578 -

:frown:Now comes the hard part.... The DREADED WAITING.
Sounds like you are on your way though.
Good luck!:sunny:


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"Freedom is something that dies unless it's used." H.T.
I've come to believe that the heart is the filter of the enlightened mind. Epilson Lyrae

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Re: First grow [Re: Fangaz420]
    #10465582 -

Congrats! I know you're totally excited, here's hoping for a good grow.

If you decide to do a follow-up batch (and you will, it gets addicting) I'd recommend constructing yourself a nice glovebox. Lysoling a glovebox is waaaaay easier than lysoling an entire room, and it has the added bonus that you can pretty much sit your stankin' ass in front of it, clean or dirty, and start working with a good chance for no contamination.

Good luck!


--------------------
My Trade List        My Epic Trip Report        My Not-As-Epic Trip Report

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Re: First grow [Re: Epilson Lyrae]
    #10465584 -

welcome to the best part

this is like the flirting before you get laid


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That did it! I am now the anti BRF CAKE! I have had enough! TO HELL WITH BRF! REAL MEN USE SEED! Go now brf younging. Get some seed. COME BACK A MAN!

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Re: First grow [Re: Fangaz420]
    #10465600 -

Were you doing PF jars?

What do you mean by too much solution?




~Monk

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Re: First grow [Re: numonkei]
    #10465616 -

Ok 1. I did make a glove box but i just wanted EVERYTHING to be sterile!

2.
Quote:
Quote:
What do you mean by too much solution?





the solution in the syringes had a lil to much trigger finger going on:P

oh and btw they are B+ and Texas

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Re: First grow [Re: Fangaz420]
    #10465641 -

What a coincidence, I've got the same two strains fruiting right now. My B+ could be doing a lot better, but I think I packed the substrate too thick in the jar (RR saw some of my abysmal jars in the contam forum and recommended I just start over). My Texas, however? Holy shit. They started growing before I even birthed them, and once they had all that fresh air they got ENORMOUS. In fact I just picked a cake clean and cloned the biggest, fastest grower into LC, which is something worth researching if you wanna take things a little farther and get them going a little quicker.


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My Trade List        My Epic Trip Report        My Not-As-Epic Trip Report

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Re: First grow [Re: Fangaz420]
    #10465885 -

You likely need not worry too much about using TOO much solution, unless you are paying for your genetics, in which case you may have been able to inoculate more substrate or if it was contaminated, in which case you're likely proper fucked anyway.

I ask what you are using as substrate because the PF jars require less sterility, (outside of inoculation, and PC or steam sterilization), than attention to detail and procedure, especially if it is a first grow. Otherwise, you may have need to be more sterile throughout your entire procedure.

Best of luck, and keep an eye out for greens and blacks.




~Monk

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Re: First grow [Re: numonkei]
    #10465926 -

i wouldnt worry too much about using a little more solution than recomended. Ive done it plenty of times when the needle got plugged with substrate and, damn solution down the side of the jar. Those jars have colinised much quicker due to a larger mass of germinating spores.

I hold the same opinion as RR, use as little spores as needed, ive used a dark syringe a few times, and even just a drop of solution was awy to many spores, those grows while colinised nicely didnt fruit too well.

I make clear syringes now and still see growth by day 3 or 4

Quote:
I ask what you are using as substrate because the PF jars require less sterility, (outside of inoculation, and PC or steam sterilization), than attention to detail and procedure, especially if it is a first grow. Otherwise, you may have need to be more sterile throughout your entire procedure.



how so?


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Re: First grow [Re: veda_sticks]
    #10465939 -

Dry vermiculite layer. Allows for a little wiggle room in working with the substrate.

Not often used in pressure cooking, as far as I know.




~Monk

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Re: First grow [Re: numonkei]
    #10465984 -

In the waiting stage atm, at one point I wouldn't leave them alone on my days off, walking round with it like a baby in my arms. Now ive seen some mycelium growth I leave them alone and coverd over and check every 1-2 days once.

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Re: First grow [Re: numonkei]
    #10466007 -

In the waiting stage atm, at one point I wouldn't leave them alone on my days off, walking round with it like a baby in my arms. Now ive seen some mycelium growth I leave them alone and coverd over and check every 1-2 days once.

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Re: First grow [Re: system32]
    #10467176 -

its the BRF tek, i pc'd 10 jars at a time at 15psi for 1 hour
i have a spare room ( pretty much grow/clean room) where i did the inoc.
lysold the whole room turned off air for an hour
entered room in tyvek coveralls and gloves wit the sleves taped down to the gloves
took a shower right before all that
flame sterilized the needle for each jar using denauterd alcohol
used GB and now i have them sitten in a tub with some holes in it with micropore tape on it, humidity is at 95%+ and they are staying around 76 degrees F

oh last thing i can really remember, i also let the jars cool overnight

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Re: First grow [Re: Fangaz420]
    #10467807 -

Letting the jars cool well enough is likely more important than most of the other steps, minus the alcohol rub and inoculation, (printing), sterilization.

Just keep an eye, you should be good. You'll probably notice quicker colonization where you used "too much" substrate.

:smile:




~Monk

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Re: First grow [Re: numonkei]
    #10467994 -

yea i know that to much is not bad and figured it would colonize faster, but i wanted 20 jars :frown::S oh well, your first time you learn A LOT of things that make/will make the second run much easier

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Re: First grow [Re: Fangaz420]
    #10469601 -

Sounds like you did a top job, now you just have to wait :tongue:

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Re: First grow [Re: spoonman_]
    #10495651 -

dont really know how to bump the thread when i update the first post, so just bumpin' it to let ya'll know whats crackin! just check first post for updates and any one tell me how to bump back to first page with just editing the first post?

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