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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: Manure lime soak?
    #92500 -

ThE JafF, you said you wanted to use calcium hydroxide to break down manure.

Wouldn't recommend it. It'll probably just destroy it.

Apparently, industrial decomposition processes are often initiated by the use of 5% H2SO4 for digestion of sugars and complex carbohydrates.

Will double-check unless anyone else out there has access to the Moonshiner's Bible. This process was used to break down raw material for the yeasts to get to work on them and produce that other drug.


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Re: Manure lime soak?
    #92502 -

Uh.... The process I am refering to involves soaking the manure in a vat of water with a small amount of hydrated lime to pasteurize it...

This is done qute commonly with straw, and it works very well.. I have no idea where you got the phrase "break down" from my post.

ThE JafF


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Re: Manure lime soak?
    #92503 -

Excellent idea JafF, good to see your still thinking all the time. I would imagine this to be excellent for the dung loving panouleous strains. I don't think it would be as effective as say heat pasteurization, but it isn't really supposed to be. Then again, old dry dung seems more resistant to contam than fresh straw in the first place.
Post results, i'm following this one. Just wondering, are you still working with peroxide in substrates, I've had a few new ideas lately, PM me if your interested.

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: Manure lime soak?
    #92504 -

Hey JafF,

I used hydrated lime in my casing as a buffer agent - and all is well. I could also soak straw in it and then innoculate?


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Re: Manure lime soak?
    #92505 -

Yup,

Scale this to your liking:

Mix 1 lb Masons lime (calcium hydroxide) in about 25 gallons of water. Place the shredded straw in pillowcases and soak the straw 16 to 24 hours. You need them completely submerged so you will have to weigh them down. Drain for 2 hours, then squeeze and squeeze. When satisfied, inoculate as usual.

Very easy.... and very good results... Have never had a batch fail... One time I waited almost 2.5 months for some oyster mushroom spawn to colonize a huge trash bag of straw. Never any contamination...

ThE JafF


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Re: Manure lime soak?
    #92506 -

That sounds really interesting. Stamets mentions it in his second cultivation book, right? But my question is this: is masons lime (calcium hydroxide) the same as hydrated lime you get for gardening?

2.5 months on straw and no contamination? Damn, lately my straw has been contaminating after ten days! And I am very carefull about temps when processing, not to mention cleanliness and all of that...


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Re: Manure lime soak?
    #92507 -

Yes this is hydrated lime.. With the 2.5 months I may have just been lucky.. I was very cautious with it, never handleing the straw, etc.. I do believe this recipe is a bit stronger than Stamets, but I could be wrong, I haven't looked at that book in a while... But it works well just the same.. I am a bit behind, everything (cambodians) is going slower than expected (although I did get some germination). I expect to test my idea sometime next week...

ThE JafF


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Re: Manure lime soak?
    #92508 -

Fucking Duh! How about this? Hydrated lime water used to make a traditional verm BRF sub. I know that straw is a lot more selective a substrate than the BR, but BR colonizes a lot quicker when spawned correctly or inoced with myc water. Here's my proposed tek, somebody please try it for me, I am suffering terribly from contaminated prints at the momment. JafF, you should recognize most of these steps as most of them were stolen from you over the years.

First, make your lime/water solution (about an ounce by weight of lime per 3 gallons water). Adding about 3 cups of H2O2 might not be a bad idea, it can't hurt. This mix will now be called limewater.

Mix your verm and BR as per standard subs (PF or MMGG) leaving out the water ofcourse. I'd say the equivalance of about 10 jars would be a good starting point.Baking the verm/BR ahead at 250 for an hour would probably help by sterilizing and making the peroxide much more effective.

In a clean blender, mix 1 colonized cake (or any appropriate spawn) with 1-2 cups water. Don't overblend, leave it fairly chunky.

Add this to the BR/Verm mix, and stir thoroughly. Add enough of the limewater to bring to the correct moisture and stir again. Put this in a suitable container, allowing for some air exchange. Perhaps a tupperware with the lid open at one corner.

There you go, what do you think? I think that the lime water and H2O2 might be enough to keep it clean.

Alternatively, you could just soak BR/Verm mix in the limewater (with H202) for a while (a day?), squeeze to propper moisture and spawn with normal dry spawn of any sort, maybe even liquid innoc.

Howzaboutzit?

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: Manure lime soak?
    #92509 -

Ok, here's my second read thoughts on that above:
First off, that comment on the peroxide being more effective was meant to be placed in the paragraph above it. Baking substrates is an effective way to destroy enzyme decomposing enzymes in traditional substrates. In the case of BR (as in most traditional subs), There are peroxide decomposing enzymes much like those in growing mycelium. These enzymes will destroy peroxide on contact. The heat pre-treatment destroys these, making the peroxide much more effective.
Secondly, I see the alternative working a little better. Granted this is just prespeculation, but I think that the longer treatment of lime would benefit the sub much more than just adding water at the time of innoc. Perhaps the first tek would benefit of letting the sub soak in limewater for a day, then innoculating ala the blender method. I think this is the best bet.
Not to draw away from JafF's original idea, just to bring up the idea of lime being used to pastuerize all subs commonly used.
peaces.

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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