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Amazon Shop for: ½ Pint Jars, Agar, HEPA Filter

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Re: Glove Box
    #90887 - 12/10/99 09:09 AM (16 years, 10 months ago)

Moe. F that make a shity hepa box. Get a Holmes hepa filter fan $45 at target and K mart "if ya want I can get the exact model NO." and a big plastic storage box $5-8. cut a space in the bottem of the box and calk the fan into thebottem of the box. I started useing it last week and it has cut down my agar contam rate I did 12 dishes and only got 2 with contam + the contamed ones were transfers from previously contamed dishes on fesh spore ones there was none. previously "without the hepa box" I did 7 and 6 were contamed and those were just spore incoulations. Good luck..

"Experence is not what happens to you it what you do with what happens to you"
A. Huxley

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Registered: 12/10/99
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Re: Glove Box
    #90889 - 12/10/99 05:48 PM (16 years, 10 months ago)

How about a HEPA-fed glove box. Build it outta 3/4" wood, use 1/4" plexiglass lid that closes on foam rubber weather-stripping. Use
brass (non-rusting) hinges,nuts,screws. Use
two cut-in-half coffee cans inserted in the front, with flexible laundry-drier hose for
your arms, duct-taped onto the other half-coffee cans (as sleeves), over which you tape
a pair of rubber gloves. Build in some wood shelves for 1/2 pint jars, a porcelin lamp
socket and energy-saver lamp, and paint the inside with glossy white Rustoleum enamel.
Cut two more holes for two half coffee cans on the sides. Stuff one with poly-fill
for the air outlet. On the opposite side use
more dryer hose to connect to a Holmes HEPA
unit using plastic indoor dryer vent attach-
ment (Home Depot) and silicon aquarium sealer. [Do not let alcholol lamp burn continuously under plexiglass]. Voila!

γνῶθι σαὐτόν - Gnothi Seauton - Know Thyself

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Re: Glove Box
    #90890 - 12/10/99 08:08 PM (16 years, 10 months ago)

If you go to a local enviromental company that removes asbestos and other haz-mats they will probably sell you what is called a glove bag they come in all different sizes it is a thick plastic bag with gloves built in and then build a box out of wood cut holes out for the arms and glue the bag (you may have to cut the bag to make it fit right) into the box and get some kind of hepa filter device I used hepa filters for respirators and an old bathroom fan I aint quit done yet but it looks good so far.

Where there is a will there is a way , sometimes it just take a little longer to get there.

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Re: Glove Box
    #90892 - 12/14/99 08:31 AM (16 years, 10 months ago)

If ya are going to use the 1/2 pint jars with agar and filter disks watch out I found out the hard way that agar can dry out fast with air exchange. + if ya do not give them an air exchaneg the build up of CO2 speeds colinization. So ya may want ot just use regular lids.

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Re: Glove Box
    #90894 - 12/15/99 10:45 AM (16 years, 10 months ago)

Hey Moe, check this out. www.sporetradingpost.com/glovebox.htm

This site has good details and even has them pre made for $50.00.

Why is it that we kill people who kill people to show people that killing people is wrong?

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Registered: 12/14/98
Posts: 129
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Re: Glove Box
    #90895 - 12/15/99 05:48 PM (16 years, 10 months ago)

I was under the impression that the metabolic processes (particularly involved in colonization) would benefit from higher levels of oxygen, considering this is the gas that fungi require for respiration.

"The universe does a math equation that never even ever really is anywhere in it, and if it spouts out creation, we're on the tip of its tongue, and it asks us where we stand." -- Modest Mouse

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Amazon Shop for: ½ Pint Jars, Agar, HEPA Filter

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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