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Offlinehappygrins
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Registered: 12/30/01
Posts: 588
Loc: Shroomery
Last seen: 17 years, 9 months
Mycelium Karo Spore Water Question
    #862833 - 09/04/02 12:44 PM (20 years, 2 months ago)


Ok, AFOF has successfully made his first  Mycelium Karo Spore Water.  He used 1/2 pint jars..  They were filled with 100 cc's of water and 1 Teaspoon of Karo Syrup, then PC'd for 20 min.  After 7 Days they all look great (Nice and cloudy).. His question is:  When Extracting this from the jars can he just suck up the water or does it specifically have to be the Mycelium Clouds?..    If so, any Idea on how to make this easier?..    When the jar is tilted to like a 45 degree angle, the clouds of mycelium seem to go to the opposite side...

Any help would be appreciated... 

Thanks everyone!..

--happygrins :grin:
 


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Offlineprimitive
newbie
Registered: 06/11/02
Posts: 38
Last seen: 4 years, 6 months
Re: Mycelium Karo Spore Water Question [Re: happygrins]
    #862964 - 09/04/02 02:28 PM (20 years, 2 months ago)

when your ready to suck up the liquid mycelium first shake the fuck out of the jar so that the mycelium is spread throughout the water.......i tried this using the honey tek and when i sucked it into my syringe i could barely see any mycelium tissue in my syringe but i inoculated anyways and i got damn good growth in about 2 days.


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OfflineBeppoMarx
old hand
Registered: 09/01/01
Posts: 1,126
Last seen: 17 years, 8 months
Re: Mycelium Karo Spore Water Question [Re: primitive]
    #863051 - 09/04/02 03:25 PM (20 years, 2 months ago)

yes i support the 'shake the fuck out of the jar' method. breaks apart the mycellium well; unless you filled your jar to the very top. and if youre worried about breaking apart the tissue- you shouldnt be. thats exactly the purpose; to distribute more strains and mix it all up.


--------------------
Holy shit people; COMMON SENSE! we were all born with it where did it go?
maybe theres a tek out there to explain how to use it!
BUCKETS BRIGADE left hand man!!!


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Offlinehappygrins
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Registered: 12/30/01
Posts: 588
Loc: Shroomery
Last seen: 17 years, 9 months
Re: Mycelium Karo Spore Water Question [Re: BeppoMarx]
    #863661 - 09/04/02 09:54 PM (20 years, 2 months ago)


Ok... thanks for the replys... this helps alot!


-happygrins :grin:


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Offlinehappygrins
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Registered: 12/30/01
Posts: 588
Loc: Shroomery
Last seen: 17 years, 9 months
Re: Mycelium Karo Spore Water Question [Re: happygrins]
    #874887 - 09/10/02 10:09 PM (20 years, 2 months ago)

One More Question on this subject...

Could a person add a Tissue sample to the Karo Water (After PC'ed and Cooled, of course) ?  ..  This would make it an Isolate, right?

Thanks..

--happygrins :grin:


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Invisiblemickey_rourke
Illegal Smile
Registered: 07/19/02
Posts: 3,333
Loc: Playa del Carmen
Re: Mycelium Karo Spore Water Question [Re: happygrins]
    #874891 - 09/10/02 10:11 PM (20 years, 2 months ago)

If you're careful and do it under sterile conditions, it would work.


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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Offlinehappygrins
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Registered: 12/30/01
Posts: 588
Loc: Shroomery
Last seen: 17 years, 9 months
Re: Mycelium Karo Spore Water Question [Re: mickey_rourke]
    #874935 - 09/10/02 10:31 PM (20 years, 2 months ago)

Cool..  will have to give this a shot then..  Why dont most people do it like this other than using  Petri-dishes / Agar agar/ ect?..

--happygrins :grin:


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Offlineblackout
 User Gallery

Registered: 07/17/00
Posts: 5,187
Last seen: 29 days, 14 hours
Trusted Cultivator
Re: Mycelium Karo Spore Water Question [Re: happygrins]
    #879121 - 09/12/02 06:35 AM (20 years, 2 months ago)

it is easier to spot contamination on an agar plate than in honey water


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