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Offlinegrod31
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help with cardboard * 1
    #7891991 - 01/17/08 07:35 AM (16 years, 15 days ago)

So i want to take a bit of colonized grain and put it onto cardboard using the cloning cardboard tek. now it says that Cardboard is semi-selective towards mycelium growth, if i added a very little bit of agar wheat malt to the water the cardboard soaks in would this make it easier for mycelium to grow/spread? if it dose i understand that it will also make it easier for contaminations to grow, but i plan on doing this completely sterile. is this step 100% completely not worth it? or should i try just for the sake of experimentation.The tek was also made for live tissue which was said to dunk in a 10% peroxide solution. is this necessary if i am using colonized wbs.and my last question is if the mycelium i want to use has been outside in 30 degree weather for 5 days is it still usable has it died, or gone into a dornmant stage?

http://www.shroomery.org/8512/Cardboard-Cloning-Tek


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Offlinedonpatch
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Registered: 11/28/07
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Re: help with cardboard [Re: grod31] * 1
    #7892005 - 01/17/08 07:43 AM (16 years, 15 days ago)

thanks for the link.. I think i forgot about that tek. I think I would still prefer the sterile enviro of an agar petri dish though. i;mt not really sure about cloning with WBS? uhm wouldnt you just want to do a g2g or maybe an g2lc?


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Offlinegrod31
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Re: help with cardboard [Re: donpatch] * 1
    #7892013 - 01/17/08 07:50 AM (16 years, 15 days ago)

haha i love to experiment with my favorite hobbies man i made a grain to grain and i made lc already.. i looked at the qt jars in my backyard and there was still colonized grain stuck to the lid so i figured why not try it...


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread


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Offlinedonpatch
purukogi...purukogi...


Registered: 11/28/07
Posts: 183
Last seen: 12 years, 11 months
Re: help with cardboard [Re: grod31] * 1
    #7892018 - 01/17/08 07:53 AM (16 years, 15 days ago)

same.. I cant stand spawn going to waste :eaglekitty:

i bet if you could get grains of wbs into those tiny tubes... put some polyfil at both ends maybe?


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InvisibleFooMan
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Re: help with cardboard [Re: grod31] * 1
    #7892029 - 01/17/08 08:00 AM (16 years, 15 days ago)

Why do you want to spawn your grains to cardboard again?

Edit: I didn't read through the whole thread. Just so you know, this isn't cloning. It's spawning to cardboard. If you want to clone, you need to use actual mushroom tissue.


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Edited by FooMan (01/17/08 08:03 AM)


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Offlinegrod31
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Re: help with cardboard [Re: FooMan] * 1
    #7892034 - 01/17/08 08:04 AM (16 years, 15 days ago)



Edited by grod31 (01/17/08 08:32 AM)


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Offlinegrod31
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Re: help with cardboard [Re: FooMan] * 1
    #7892050 - 01/17/08 08:12 AM (16 years, 15 days ago)

Really, no reason other then i haven't used cardboard before, dose that mean the questions shouldn't be answered? i asked nice:smirk:

" this isn't cloning. It's spawning to cardboard. If you want to clone, you need to use actual mushroom tissue. "-- i never called it cloning i just said i used cardboard cloning tek, c'mon im not a noob like that. if you could answer my questions the it would be great though.


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread


Extras: Filter Print Post Top
InvisibleFooMan
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Re: help with cardboard [Re: grod31] * 1
    #7893970 - 01/17/08 05:04 PM (16 years, 15 days ago)

I'm not sure why you're all pissy over my comment. It wasn't a stab at you. To answer your main question, yes I don't see why it wouldn't work. But even if you aren't cloning, it still doesn't make much sense to me that you would bother doing that. Now if you wanted to spawn your seed to a BIG pile of wet cardboard and see what would happen, your experiment might seem a little cooler.


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Quick WBS Prep


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Offlinegrod31
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Registered: 12/06/07
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Re: help with cardboard [Re: FooMan] * 1
    #7894827 - 01/17/08 08:31 PM (16 years, 15 days ago)

see that would be a waste of grain to me.. im using a total of 40 colonized grains stuck to the top of a jar to do 4 peices of cardboard to see if it would work... i have never read of using an agar mixture with cardboard.


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread


Extras: Filter Print Post Top
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