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Andrew47
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Registered: 04/06/06
Posts: 432
Last seen: 14 years, 3 months
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Wild Ganoderma Applanatum
#7435554 - 09/21/07 07:27 AM (16 years, 4 months ago) |
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Hey Guys,
I picked some artist conks yesterday. One of them I broke in half and took a soft-ish (as soft as it gets in there) chunk that was somewhat white from near where it attached to the tree inside the glove box. There I split it again and from the center I put little pieces to three dishes of agar.
Afterwards, I put them whole or in halves into a big pot and boiled for four hours. Then I put the pot in the fridge where it still sits with the mushrooms inside there.
Has anyone else here cloned polypores with this method? Is it possible for it to work? It was the only notably soft flesh inside the mushroom, the rest was so thick almost like wood. The tissue was still quite fibrous, nothing like the usual fruits we know.
Also, was boiling for 4 hours a reasonably purposeful extraction from the mushrooms? I still have them and like I said they've sat overnight in the fridge in the water. Should I boil it some more or add some vinegar?
Many thanks & Kind Regards
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RogerRabbit
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Wild Ganoderma Applanatum [Re: Andrew47]
#7437209 - 09/21/07 03:46 PM (16 years, 4 months ago) |
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You might boil it one more time. We slice them into wafers 1/4" thick. A saw works well for this. These thin wafers help the medicine to extract easier and quicker.
If the clone doesn't take, pick another one and dig upwards from the spore side, almost at the very leading edge. You should find the white fleshy part on the bottom of the conk easy to cut. With G applanatum, I just cut out a chunk as said above, and then when in front of the flowhood, I'll tear that in half and clone some freshly exposed tissue. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Andrew47
Servant of allLife



Registered: 04/06/06
Posts: 432
Last seen: 14 years, 3 months
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Re: Wild Ganoderma Applanatum [Re: RogerRabbit]
#7443710 - 09/23/07 10:48 AM (16 years, 4 months ago) |
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Hey Roger,
Thank you very much for your information.
The tea came out very dark after I reduced it. How long do you think it'll keep in the refrigerator?
Also, the clone looks perfect. I made three plates, the first two were mess-ups and the third was a good one. My "scalpel" (exacto-knife) wasn't nearly sharp enough for the job. I broke off that white chunk in the glovebox and then tore it in half, but the tops and bottoms had been exposed to air outside the govebox for a minute in my oust-ed but otherwise dirty kitchen. The first two plates ended up getting pieces that had parts that had been on the outside because I couldn't really cut the top and bottom off very easily. The third managed to come out perfectly.
The third plate has a 1.5mm x 3mm piece right in the center. Today it is showing no contamination and looks like healthy mycelium slowly growing from the tissue.
The other two, one looks fine starting to grow but the other is possibly contaminated. I'm going to toss these two since the third looks so perfect.
Thanks again for the help
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RogerRabbit
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Wild Ganoderma Applanatum [Re: Andrew47]
#7443973 - 09/23/07 12:31 PM (16 years, 4 months ago) |
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I'd suggest making a couple of quick transfers of tissue from the good plate. This will ensure that if mold sporulates in the mother dish later, you'll have removed healthy mycelium first. I'll easily go through a whole sleeve of 20 petri dishes to isolate a couple of pure cultures. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Andrew47
Servant of allLife



Registered: 04/06/06
Posts: 432
Last seen: 14 years, 3 months
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Re: Wild Ganoderma Applanatum [Re: RogerRabbit]
#7444592 - 09/23/07 04:00 PM (16 years, 4 months ago) |
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Hi RR,
thank you for the tip. I have to do some other transfers tomorrow, I will transfer it also.
-------------------- It's easy! Send your clean prints to www.fsrcanada.com
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Andrew47
Servant of allLife



Registered: 04/06/06
Posts: 432
Last seen: 14 years, 3 months
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Re: Wild Ganoderma Applanatum [Re: Andrew47]
#7479805 - 10/03/07 05:54 AM (16 years, 3 months ago) |
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Hey guys,
There's mycelium growing out of the old mushrooms! I left them outside one in the grass and one on some mulch and it's rained kinda heavily the past few days. They've shrunken some and now ropy myc is popping up all over them
-------------------- It's easy! Send your clean prints to www.fsrcanada.com
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