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LC from print - putting the whole print in
    #7354852 -

I had a small, faint print which I wanted to make LC out of. Instead of trying to scrape spores from it into the LC, I cut off a piece and put it into the LC. So there's a bit of paper with spores on floating around in the LC.

7 days later one of the 2 LCs I did like that I can't tell if there's growth or not and the other I can't see any growth at all. Is there any reason why this method wouldn't work? I can see that the chance of contamination could increase. But I can't see why spores on paper would have any trouble germinating when soaked in honey water.

Has anyone tried this?

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Re: LC from print - putting the whole print in [Re: roquet]
    #7354884 -

Seven days is not that long for spores, depending on temps and the actual LC. Wait a few more days before you start to worry. At least you'd know if you had contams by now, so that's good.


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Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.

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Re: LC from print - putting the whole print in [Re: Dr_T]
    #7355184 -

ive been asking alot about this latley too. (not putting paper in the lc) but jus putting spores off a print directly instead of making a syringe. and yes the contam rate is higher but if its done in a glove box under sterile conditions there is no reason why that shouldnt work. they say around 2 weeks is when you should start seeing growth.

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Re: LC from print - putting the whole print in [Re: blood4blood]
    #7355289 -

Hey there,

This method has worked for me before, the only issue is if the print and paper truly is sterile, excluding the spores of the mushroom of course


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It's easy! Send your clean prints to www.fsrcanada.com

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Re: LC from print - putting the whole print in [Re: blood4blood]
    #7356817 -

Quote:
blood4blood said:they say around 2 weeks is when you should start seeing growth.



2 weeks is a long time to only "start" seeing growth. I did another LC at the same time with a dark print on foil where there was no problem scraping off spores and had clearly visible growth in 5 days.

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Re: LC from print - putting the whole print in [Re: roquet]
    #7357297 -

I recently started some LC from a syringe- hydrated spores- and it took a week to see anything, and after ten days there isn't much. Every strain, and every mushroom, is different.


Hang on, I'll go check it again... ok. Inoc on 8/20, today is 8/31. Eleven days, and it's just now got enough growth that I might be able to use it on jars, probably better to wait a few more days. And again, that's from already-hydrated spores. So you aren't that far out.

Now, start a batch of LC using mycelium, and watch it explode. Either a few drops of another LC, or some other source. Blammo!


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Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.

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