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InvisibleCLuB99
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Registered: 10/27/99
Posts: 1,316
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Re: What's the verdict on freezing spores, then??
    #73101 - 01/27/00 10:13 PM (22 years, 8 months ago)

I'm waiting spores from Ryche to.... :eek:
I know that a solution 10%/90% glycerol/water can stores the spores at very low temps for years..but i don't know if Ryche use some storing agent in his syringes..


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Offlinesutec
addict
Registered: 09/06/99
Posts: 301
Last seen: 13 years, 3 months
Re: What's the verdict on freezing spores, then?? [Re: CLuB99]
    #73103 - 01/27/00 10:59 PM (22 years, 8 months ago)

I wouldn't worry TOO much about freezing. Unless they're going to be frozen for LONG periods of time (weeks? months?) I don't think that there would be too much of a problem. Especially since IF they do freeze they wouldn't be frozen for longer than a day or two (unless you let your packages just sit outside...)


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Anonymous

Re: What's the verdict on freezing spores, then?? [Re: CLuB99]
    #73104 - 01/28/00 02:20 AM (22 years, 8 months ago)

Spores consist of an outer husk, a tiny amount of oil, and a package of DNA. They can survive in the absolute vacuum and hard radiation of interstellar space. Cooking will kill them, but overall, they're pretty resilient.

There was an experiment done in the 1930s where spores were frozen in liquid nitrogen, then every so often, a bunch were defrosted and germinated. Fine crop, every time.

If they can take liquid nitrogen for thirty years, you needn't worry about it being a bit chilly...



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OfflineFonz
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Registered: 07/26/99
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Last seen: 20 years, 7 months
Re: What's the verdict on freezing spores, then?? [Re: CLuB99]
    #73105 - 01/28/00 04:18 AM (22 years, 8 months ago)

A few months ago my girlfriend thought the milk was going bad to often and cranked the cold up on the fridge, freezing everything including all my spores. They were frozen solid inside the syringe. I happy to report that it appears not to have affected them at all. So nope, no need to worry.


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InvisibleRyche HawkV
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Registered: 03/01/01
Posts: 2,112
Re: What's the verdict on freezing spores, then?? [Re: CLuB99]
    #73106 - 01/28/00 06:33 AM (22 years, 8 months ago)

.


--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com



muShrooms.com is the new web site of
www.thehawkseye.com


Edited by Ryche Hawk (06/06/17 03:12 AM)


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Anonymous

Re: What's the verdict on freezing spores, then?? [Re: CLuB99]
    #73107 - 01/28/00 08:13 AM (22 years, 8 months ago)

Good info there. Maybe a tougher question: would freezing spores be better for long-term storage than just putting them in the refrigerator?

I know its not ideal, but if someone had some extra syringes and couldn't use them so fast, just curious..



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InvisibleRyche HawkV
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Registered: 03/01/01
Posts: 2,112
Re: What's the verdict on freezing spores, then?? [Re: CLuB99]
    #73108 - 01/28/00 11:30 AM (22 years, 8 months ago)

.


--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com



muShrooms.com is the new web site of
www.thehawkseye.com


Edited by Ryche Hawk (06/06/17 03:12 AM)


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