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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Strain Isolation Methodology
    #7301588 -

Greetings. I have a simple quick question about cloning/strain isolation.

One of my books states in detail to clone using the tissue (mycelium) inside the stipe.

Then I have an older video reference telling me to split the cap open and that the best sample for tissue culture being the inner cap?

What one is the best? My gut tells me the stipe, due to actual visual mycelium growth.

If you have an opinion on your preferred method please share it.

All sterile techniques will be used, laminar flow hood, sterilizing the mushroom to be cloned via alcohol. ect ect...so don't bother with chatting about that. thanks.


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Re: Strain Isolation Methodology [Re: primordialoddity]
    #7301630 -

The stipe seems most logical to me. For one it's just easier to work with, easy to handle, the outer tissue peels back to revel the inner tissue... second there is less change of accidentally "contaminating" your clone with spores from the cap.

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Re: Strain Isolation Methodology [Re: musher_420]
    #7301646 -

Yeah my thoughts exactly, I was thinking the same thing...earlier in the video the guy explains IN DEPTH how spores spreading in the lab is horrible and bad talking about agar transfers and stuff after that...then he fucking tells you to take your tissue sample from the cap.

good point.

stipe'n fersure.


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...to unite the powers of the conscious and unconscious, of intelligence and emotion.

NOTE: All posts and/or messages from this account are 100% fictional and should not be taken literally.

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Re: Strain Isolation Methodology [Re: primordialoddity]
    #7301861 -

Contaminants are always more readily found outside and surrounding exterior tissue than they are inside an organism. So the most logical thing to do would be to take a core sample from the stipe.

Peace

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Re: Strain Isolation Methodology [Re: musher_420]
    #7304006 -

musher_420 said:
Second there is less change of accidentally "contaminating" your clone with spores from the cap.


Thats exactly what I thought as soon as I read this

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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