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 Toxicman23
Stranger
Registered: 06/19/06
Posts: 9
Last seen: 17 years, 7 months
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 Inoculing by polyfil filter and jar shaking 
#5922634 - 08/01/06 06:01 PM (17 years, 8 months ago) |
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Hi all
Wanna ask you guys if it s possible to inoculate through the polyfill filter or i better have to make a second small hole for that ?
other subject I plan to make popcorn jars should i shake them every 4 days or just let them colonized ?
thanks for you r help 
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stormhalter
full metalalchemist
Registered: 07/10/06
Posts: 175
Loc: Behind you.
Last seen: 16 years, 8 months
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 Re: Inoculing by polyfil filter and jar shaking [Re: Toxicman23]
#5922683 - 08/01/06 06:20 PM (17 years, 8 months ago) |
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Inoculate through a 1/4" hole filled with Polyfill.
Shake jars around 30% colonized, and leave alone until 100%
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Yamidude
Stranger
Registered: 06/15/06
Posts: 957
Last seen: 15 years, 7 months
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Re: Inoculing by polyfil filter and jar shaking [Re: stormhalter]
#5922705 - 08/01/06 06:25 PM (17 years, 8 months ago) |
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i innoc through the polyfill on mine..
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 Tippinthru
contented
Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: Inoculing by polyfil filter and jar shaking [Re: Toxicman23]
#5922772 - 08/01/06 06:41 PM (17 years, 8 months ago) |
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Rye, as a substrate, or WBS as spawn would be a better choice than popcorn.
Tyvek would be a better choice than polyfil.
2 holes, one on either side of the lid.
Either place tyvek under the ring, and over the lid.
Or, tyvek under the lid, you choose.
Once PC'ed, allow to cool to room temperature (overnight).
Inoculate through one hole, then cover that hole with Micropore tape.
Simple, easy & effective.
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Perfection is attained by slow degrees; it requires the hand of time...
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Toxicman23
Stranger
Registered: 06/19/06
Posts: 9
Last seen: 17 years, 7 months
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Re: Inoculing by polyfil filter and jar shaking [Re: Tippinthru]
#5926286 - 08/02/06 06:10 PM (17 years, 8 months ago) |
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All right guys thanks a lot for your help
Tippinthru i have try one time rye but it was horrible. I ve followed the tek carefully but dunno what happened it seem to have a brow juicy jelly crap in the jars  That s why i want to try with popcorn :p
@++
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monstermitch
Growing in Bags Doesn't Work
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay 
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Re: Inoculing by polyfil filter and jar shaking [Re: Toxicman23]
#5926363 - 08/02/06 06:35 PM (17 years, 8 months ago) |
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Quote:
Toxicman23 said:
All right guys thanks a lot for your help
Tippinthru i have try one time rye but it was horrible. I ve followed the tek carefully but dunno what happened it seem to have a brow juicy jelly crap in the jars That s why i want to try with popcorn :p
@++
I believe will end up shooting yourself in the foot.
see, you just described a bacterial infection from rye.
either you didn't soak long enough,
or pc long enough,
or your breath contaminated your inoculation,
or your incubation temps could have been over 86 degrees.
popcorn is the last grain to choose if you are looking to avoid bacterial endospores.
you probably run a higher risk of the same thing happening to you again
with popcorn, than you do with any other choice of spawn.
even a second try with rye.
personally, I suggest finding a good wbs tek and giving that a go.
good luck to you.
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: Inoculing by polyfil filter and jar shaking [Re: monstermitch]
#5926388 - 08/02/06 06:42 PM (17 years, 8 months ago) |
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Quote:
monstermitch said:
popcorn is the last grain to choose if you are looking to avoid bacterial endospores.
never had the problem with popcorn, WBS it's happened more often but gypsum can help to offset the chances of bacteria contams whe used in conjunction with proper draining
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monstermitch
Growing in Bags Doesn't Work
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Re: Inoculing by polyfil filter and jar shaking [Re: Prisoner#1]
#5926395 - 08/02/06 06:44 PM (17 years, 8 months ago) |
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I'm the opposite.
go figure.
but I do use gypsum every time I soak my wbs.
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FungusMan
I81U812
Registered: 08/06/05
Posts: 3,112
Loc: Everywhere
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Re: Inoculing by polyfil filter and jar shaking [Re: Prisoner#1]
#5926400 - 08/02/06 06:46 PM (17 years, 8 months ago) |
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I innoc mine through my polyfill. Before doing that, I would make these overcomplicated jars up (pic 1). Now, I just flame sterilize, then innoc thru the poly (pic 2).
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Toxicman23
Stranger
Registered: 06/19/06
Posts: 9
Last seen: 17 years, 7 months
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Re: Inoculing by polyfil filter and jar shaking [Re: FungusMan]
#5926494 - 08/02/06 07:12 PM (17 years, 8 months ago) |
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Ok i will go for polyfill lids with in Fungusman inoc process
Will do half popcorn and half wbs
I ve a last question
Do i better have to flame the needle and put it asap though the polyfill and wait for it to cool then inoc or just flame wait and inoc ?
I m worried that if i wait for the syringe to cool out the jars crap will fall on the needle during the cooling time is that possible ? 
Tanks for your help 
Edited by Toxicman23 (08/02/06 07:18 PM)
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: Inoculing by polyfil filter and jar shaking [Re: Toxicman23]
#5926580 - 08/02/06 07:32 PM (17 years, 8 months ago) |
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One of the secrets of grain preparation, regardless of which grain, is to rinse the kernels very well before continuing with whatever preparation tek you're going to use. All grains get packaged with a lot of chaff and grain dust that if not rinsed out will cause the grains to stick together later and cause that sticky goo.
Put your dry grains in a large kettle and fill with water as you stir it around. You'll see all the dust and other crap come floating up. Slowly dump out the water to get rid of the junk and repeat two more times or until the water pours off clear.
Adding gypsum as prisoner1 said is also a great idea.
It will take anyone a few tries to get the moisure content right, regardless of which grain or which tek you follow. If it's too wet this time, make adjustments next time, and so on until you work out your system.
Flame your needle and use it right away. The first drop or two of solution will cool the needle without wasting too many spores. As said, if you hold it until cool, it's likely to get contaminated again. I feel it's best to sacrifice a few spores/solution to avoid the problem.
RR
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