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Offlinecookiewhore
enthusiast
Registered: 01/03/02
Posts: 385
Last seen: 21 years, 10 months
h2o2 effect...
    #565929 - 02/28/02 02:17 PM (21 years, 11 months ago)

AFOAF has a big problem. He's paranoid about contam in his rye grain jars. There are web-like greyish filaments in most / all of his jars. He put a couple into fruit with case, and the case looked contam'ed. We looked at all of his cultures, and some that looked shady a couple days ago are nice and white now. We shook them this morning and injected some peroxide. I was under the assumption that any bubbles seen means comtam. Every single culture bubbled like crazy!! Does healthy mycelium bubble after the network has been shaken ? I injected peroxide on top before shaking ... no bubbles. I shook the jars and they bubbled like mad. Anyone know what's going on ?? Cobweb is suspected. Does cobweb bubble with peroxide ?? thanks!!

ps since posting this in 'cultivation', (to which noone read OR replied ..) i've since injected my 'healthy' myclilial cultures (agar) with some peroxide .. which bubbled. i have half a mind to think either peroxide does in fact bubble with pure mycilium, OR there are contams UNDER (? is this possible?) the mycilium on top or in the agar. any experience with this ?? THANKS!


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OfflineKaren
Pooh-Bah
Female

Registered: 01/02/00
Posts: 1,677
Last seen: 6 years, 8 months
Re: h2o2 effect... [Re: cookiewhore]
    #568889 - 03/03/02 05:54 PM (21 years, 11 months ago)

I would imagine that you would see some mild bubbling with mycelia. H2O2 hurts the mycelia to a certain degree when you use it but it can withstand the damage that it sustains.

I don't know for sure or not but I am giving you my opinion.

Karen


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