Home | Community | Message Board



Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Jump to first unread post. Pages: 1
Invisiblecaptain_beyond
tuba player
Registered: 10/09/04
Posts: 11
what is peroxide dunking?
    #3252749 - 10/19/04 07:58 PM (12 years, 1 month ago)

I've read in several posts about a "peroxide dunking" technique for keeping bacteria at bay. Can anyone describe this procedure for me or give me a link somewhere? I didn't find a tek on the shroomery or in the posts for this procedure.


--------------------
everybody's got something to hide, except me and my monkey


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,275
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 6 months, 7 days
Re: what is peroxide dunking? [Re: captain_beyond]
    #3254997 - 10/20/04 06:49 AM (12 years, 1 month ago)

"peroxide dunking" is a disinfecting/cleaning technique. It consists on creating a diluted solution of h2o2 (peroxide) and h2o (water), followed by a mycelium dunk in that solution for a period of time. The element ratios may vary, depending on the peroxide concentration. If you use the 3% variant, it should be 1 portion of peroxide to 10 portions of water (1:10).
The dunking process should last between 24h to 48h but it can be done in less time if the contamination is small. After the dunk, you can place the cakes on the terrarium.
I normally use a relatively large and clean tupperware with a secure cover.
Anyway, i don't think peroxide dunk is the best suitable process to fight bacteria. You should use bleach dunk instead, the process is exactly the same but the ratios are different because bleach is much a stronger disinfectant, you can use 1:100 (bleach:water) or 1:200 (bleach:water) ratio depending on the strength of the contamination.
Just don't mix bleach and peroxide, they form a bubbling reaction when mixed.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblecaptain_beyond
tuba player
Registered: 10/09/04
Posts: 11
Re: what is peroxide dunking? [Re: MAIA]
    #3267654 - 10/23/04 02:08 PM (12 years, 1 month ago)

Just out of curiosity, does any one know why the H2O2 doesn't harm the mycelium? It sounds like a chemistry problem.


--------------------
everybody's got something to hide, except me and my monkey


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepacopaco
Mad Hatter

Registered: 09/12/04
Posts: 82
Loc: Upstate New York
Last seen: 11 years, 10 months
Re: what is peroxide dunking? [Re: captain_beyond]
    #3271442 - 10/24/04 03:01 PM (12 years, 1 month ago)

many living cells, including fungi, have an organelle in there cells called a peroxisome, that produce an enzyme that decomposes hydrogen peroxide into water and oxygen. (Hydrogen peroxide may be one of the wastes produced by cellular respiration - so there's gotta be a way to digest it...)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,275
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 6 months, 7 days
Re: what is peroxide dunking? [Re: pacopaco]
    #3279702 - 10/26/04 03:49 PM (12 years, 1 month ago)

Right.
Just to add that mushrooms have a more complex structure than most occurring contaminants and can break down h2o2 in water and oxygen because of that enzyme. Bacteria has a similar enzyme, that's why it's so hard to fight off sometimes.
Anyway, of course the enzyme production is limited and mycelium can also subside if the exposition to that medium takes too long. Also, the concentration of the medium (h2o2) plays a role in the whole equation.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Similar ThreadsPosterViewsRepliesLast post
* peroxide dunk question evilchipmunk 1,052 3 08/29/04 08:00 PM
by evilchipmunk
* Hydrogen Peroxide dunking before casing to kill contams JohnnyRespect 750 1 04/24/03 10:31 AM
by MAIA
* Dunking With Peroxide ComfortablyStond 600 1 04/01/06 12:15 PM
by MLBjammer
* Hydrogen Peroxide wet spot treatment: NEW IDEA!! ShroomInduced 2,107 8 05/03/05 05:39 AM
by ShroomInduced
* Cobweb on fully colonized cakes after dunking? is it possible? Cham 1,162 2 05/21/06 04:50 PM
by RogerRabbit
* hyrdogen peroxide diabla 819 6 07/05/05 09:22 AM
by BetaDelta
* black pin mold on peroxidated MEYA YidakiMan 1,637 12 09/15/03 11:41 PM
by YidakiMan
* Cobweb meets peroxide, At first you see it, now you don't Eraserhead 3,066 7 09/25/06 07:51 AM
by Eraserhead

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte
1,447 topic views. 0 members, 4 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Zamnesia.com
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.003 seconds on 14 queries.