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 neversummersux
Stranger
Registered: 01/30/02
Posts: 2
Last seen: 15 years, 7 months
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 Myco bags with puerto rican spores...NEED Help! 
#544255 - 02/07/02 11:43 PM (15 years, 7 months ago) |
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I bought four myco bags and a syringe of puerto rican spores and started them exactly a month ago. i did exactly what the directions said. inject the spores, mix the medium, and cover the hole with tape. after i did that, i placed all 4 bags back in the shipping box and closed the lid and moved them into a warm, dark closet as the instructions said. i noticed mycelial growth about 5 days later, and it seemed like it was going well until the growth stopped at about 3 weeks. the directions said puerto rican spores take about 18 days to colonize a quart jar, but the bags aren't even close to a quart. nothing appears t be wrong with them, like infection or mold, etc. some of the bags are more colonized than others, but none are 100% colonized, the one that is most colonized is about 75%. i am here about a month later and wondering what i may have done or can do to get things back on track. i placed a heating pad under the box to help regulate temperature. it chills at about 75 degrees right now. i hope its not too late!!
PLEASE HELP!!! an help would be greatly appreciated..
thanx,
a newbie
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Workman
Psilocybe Microscopist
 
Registered: 03/01/01
Posts: 3,508
Loc: Washington, USA
Last seen: 15 hours, 12 minutes
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 Re: Myco bags with puerto rican spores...NEED Help! [Re: neversummersux]
#544717 - 02/08/02 12:32 PM (15 years, 7 months ago) |
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Interesting. No prior reports of stalling with the mycobags. It doesn't sound like you are mixing the bags. Try breaking up the colonized area and mixing it up being careful not to rupture the bag or dislodge the tape. Keep them warm and the bags should be fully colonized in another week or less.
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Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification  
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Supershrooomer
journeyman
Registered: 11/02/01
Posts: 112
Last seen: 7 years, 10 months
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Re: Myco bags with puerto rican spores...NEED Help! [Re: Workman]
#548108 - 02/11/02 05:59 PM (15 years, 7 months ago) |
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I also have 1 B+ bag that is stalled at about 80%. What could be causing this?? Any suggestions workman?
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windex
old hand
Registered: 06/27/01
Posts: 1,293
Last seen: 3 years, 3 months
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Re: Myco bags with puerto rican spores...NEED Help! [Re: Supershrooomer]
#548225 - 02/11/02 07:46 PM (15 years, 7 months ago) |
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Ive found that everything for the PR strain needs to be perfect. Ive gotten two syringes of PR from sporeworks and 4-5 others from sporeworks and with both PR's i either got no growth or such slow colonation.
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 3,508
Loc: Washington, USA
Last seen: 15 hours, 12 minutes
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Re: Myco bags with puerto rican spores...NEED Help! [Re: Supershrooomer]
#548335 - 02/11/02 10:38 PM (15 years, 7 months ago) |
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In my experience with grain spawn, bacteria can cause stalling after nearly complete colonization. Does the bag appear excessively wet? Did you mix the substrate and then get no recovery?
If the bag appears normal but the growth has unexpectedly slowed to nearly stopped and you haven't mixed, then its probably just poor gas exchange. Mixing the substrate should solve that. If its contamination, then the bag might be a loss. If you suspect bacteria, you can try to lower the temperature to around 60-65F or lower and hope the mycelium can overgrow the bacteria (which it often can at lower than optimum temps with a good percentage of the substrate already colonized) but it takes a long time.
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Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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Splat
Deviant
Registered: 01/31/02
Posts: 80
Loc: Santa Cruz CA
Last seen: 11 years, 7 months
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 Re: Myco bags with puerto rican spores...NEED Help! [Re: Workman]
#548367 - 02/11/02 11:02 PM (15 years, 7 months ago) |
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Shifting the bag will probably help, in my batch I also uped the heat a bit for colonization to about 80-85, this seemed to help... they did take longer than average to colonize though..
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You know what I always say....anything worth doing.... mmm....is worth overdoing!
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