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Offlinegray1
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field culturing
    #524665 - 01/18/02 04:43 PM (15 years, 10 months ago)

what is the best way to culture a mushroom that you see collect in the forest withough taking a spore print and germinating spores?
can a tissue sample be taken and transfered to agar/liquid media?


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Invisiblemycofile
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Re: field culturing [Re: gray1]
    #524718 - 01/18/02 05:40 PM (15 years, 10 months ago)

Yup, carry some peroxidated agar slants and a bottle of diluted peroxide with you. Cut a piece from inside the base of the stem. Hold the tissue sample with a pair of forceps, needle, or the like. Pour the peroxide over, and then drop it in the peroxidated agar slant.

You can get by just dropping a piece of the stem in the slant, but I used to wash it first anyway.


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Offlinegray1
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Re: field culturing [Re: mycofile]
    #528025 - 01/22/02 10:06 AM (15 years, 10 months ago)

gracias. would this work for other fungi, not mushrooms alone? i assume yes given the fungi like the nutrient media you supply.


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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