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gray1
addict

Registered: 04/30/01
Posts: 430
Loc: brooklyn
Last seen: 21 years, 5 months

field culturing

#524665 - 01/18/02 02:43 PM (21 years, 8 months ago)

what is the best way to culture a mushroom that you see collect in the forest withough taking a spore print and germinating spores?
can a tissue sample be taken and transfered to agar/liquid media?

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mycofile
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Registered: 01/18/99
Posts: 2,336
Loc: Uranus

Re: field culturing

[Re: gray1]
#524718 - 01/18/02 03:40 PM (21 years, 8 months ago)

Yup, carry some peroxidated agar slants and a bottle of diluted peroxide with you. Cut a piece from inside the base of the stem. Hold the tissue sample with a pair of forceps, needle, or the like. Pour the peroxide over, and then drop it in the peroxidated agar slant.

You can get by just dropping a piece of the stem in the slant, but I used to wash it first anyway.

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gray1
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Registered: 04/30/01
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Last seen: 21 years, 5 months

Re: field culturing

[Re: mycofile]
#528025 - 01/22/02 08:06 AM (21 years, 8 months ago)

gracias. would this work for other fungi, not mushrooms alone? i assume yes given the fungi like the nutrient media you supply.

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Mushrooms, Mycology and Psychedelics > Advanced Mycology

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