Home | Community | Message Board



Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, Malt Extract, Paul Stamets

Jump to first unread post. Pages: 1
Offlineupupup
guardian

Registered: 08/25/01
Posts: 889
Loc: George "I love Hitler" Bu...
Last seen: 13 years, 27 days
Questions about MEA and peroxide...
    #1075086 - 11/21/02 10:56 PM (14 years, 4 months ago)

In TMC H2O2 is not talked about in connection with Agar. I know that the H2O2 would kill the spores but not live tissue.

Is there some reason why they recommend getting pure strains on just plain old MEA rather than MEA w/h2o2?

Since my monkey is new at MEA he thinks he sees MEA w/out h2o2 grows faster but is obviously a contam waiting to happen. Am I seeing this right? Does straight MEA grow faster than MEA w/h202?

If I got this all messed up then why does TMC not talk about h2o2 MEA?


--------------------
Support bacteria - they're the only culture some people have.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Questions about MEA and peroxide... [Re: upupup]
    #1075883 - 11/22/02 04:53 AM (14 years, 4 months ago)

A pure strain has no need for the protection of H202. TMC assumes that asceptic technique is followed durring agar work. The isolation of a pure strain from a contaminated print would be easier with the use of H2O2. I believe the book calls for other methods such as the use of antibiotics to rid a culture of bacteria. Otherwise, isolates from mold contaminated cultures are fairly easy to get if you follow proper technique.

When growing out two cultures, one w/ H202 and one w/o, I would expect initial growth on the H2O2 to lag while catalases and peroxidases are upregulated. Some species do not do well on peroxidated agar, this may be the reason it is not mentioned in TMC.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,122
Loc: my room
Last seen: 8 hours, 27 minutes
Re: Questions about MEA and peroxide... [Re: Joshua]
    #1075929 - 11/22/02 05:41 AM (14 years, 4 months ago)

>this may be the reason it is not mentioned in TMC

The reason is that at the time TMC was published(1983) the use of peroxide in mushroom cultivation hasn?t been "invented" yet.

More than 10 years later Rush Wayne published his manuals.

More info:
www.mycomasters.com


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineupupup
guardian

Registered: 08/25/01
Posts: 889
Loc: George "I love Hitler" Bu...
Last seen: 13 years, 27 days
Re: Questions about MEA and peroxide... [Re: Anno]
    #1076455 - 11/22/02 11:36 AM (14 years, 4 months ago)

Hmm. Right arm. Thanks kids...


--------------------
Support bacteria - they're the only culture some people have.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Questions about MEA and peroxide... [Re: Anno]
    #1077814 - 11/22/02 09:46 PM (14 years, 4 months ago)

I thought about that, but was unaware of when Wayne's work was published.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar, Malt Extract, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Unsteralized Peroxide Growing puwtrip 2,897 10 05/15/05 12:47 AM
by Snarp
* Peroxide FAQ YesItsMe 958 6 05/24/04 01:38 PM
by Jopo
* Dr Rush Wayne's Peroxide sterilisation process .. !!! InfiniteAdam 3,348 12 11/17/03 03:03 PM
by mycofile
* Problems getting Rhizo growth from MEA
( 1 2 all )
upupup 3,153 32 12/05/02 08:44 AM
by stonErollEr1
* How to mix Peroxidated MEA Richard_D_James 1,562 7 03/27/02 04:43 PM
by ParticleMan
* Several Psilocybe cyanescens questions LordByron 5,925 16 01/04/08 11:28 PM
by LordByron
* the peroxide manual fungusamongus 1,677 10 10/22/01 10:28 PM
by dimitri211
* Peroxide Method(bulk) verticalclone 988 2 12/13/01 09:08 AM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
978 topic views. 0 members, 6 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Crestline Sales - MycoPath
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.037 seconds spending 0.005 seconds on 14 queries.