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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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new to shiitake...please help from a pro
    #4760964 -

hi..i had recently took clones to grain(popcorn) of a shiitake. the grain is now fully colonizes. now i am in need of help in step by step proceedures to the next level. i know it needs wood base substrates but what additives and or proceedure do i need to make this a succeed.
thanks
peace
siam

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Re: new to shiitake...please help from a pro [Re: Thai_connection]
    #4763934 -

ok...no answers, maybe i need to re-phrase my questions...i want to make mycro bags. what i have are hard wood shavings, sawdust and coir evenly mix and soaked 12 hour in very warm water with 10% bleach. then I dumped the water out and now it is soaking in 1 cup of 6% H2o2 to 5 gallon of water.
i also have in a separate container some unhusk rice soaking in h202.
i also have gypsum, magnesium sulfate and lime which i will surely use.
now would it be a good idea to mix in the unhusk rice and pc it with the mix? and to the last question (promise) is i have a spawn to innoc the bags. can i just break out the grain and add water and shake it up then suck it up with a syringe and shoot it or would it be better to just mix the grain with the wood shaving mix?
sorry for all the questions..kinda new to all these stuff, i want to do it right and not go thru unneeded waste and cost.
peace
siam

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Re: new to shiitake...please help from a pro [Re: Thai_connection]
    #4765122 -

I'm afraid that much bleach will prevent the mycelium from growing. I would recommend you start over. Soak the woodchips in plain water with perhaps 1% molasses added. A tablespoon of gypsum per gallon of soak water is also good. Soak the wood chips for a day or two, and the sawdust for a few minutes. Load into your bags and sterilize in a PC for two hours or so. When cool, inoculate. Leave in the bags for a week or so after full colonization, then stick in the refrigerator for 48 hours while still in the bag. After that, remove the bag and place in fruiting conditions, or weather permitting, move the block outside to a place in the shade to fruit.
RR


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Re: new to shiitake...please help from a pro [Re: RogerRabbit]
    #4765955 -

Growing shiitakes - the first and most important thing is strain. If you have a really good strain, it will fruit no matter what you do. But most strains are not that easy to fruit. If you want to use a chemical sterilant, H2O2 does not work well and will give you deformed fruit bodies. Don't waste your time on that one. But bleach is good, very good. However, you need to get rid of the bleach residue. The best way to do that is to add some Sodium thiosulfate to the soak water, wait 20 minutes, then drain your chips. The sodium thiosulfate causes the chlorine to outgas and leave the water/wood chips. Inoculate by adding grain spawn to the chips. If you use grain spawn, just add a lot and you can get by with no amendments. If you use sawdust spawn, you will need to add a secondary source of nitrogen, like 5-10% bran. Rice, wheat or oat bran works good. Also dry catfood or dogfood works GREAT if you have it. Keep in mind, the more spawn you use, the greater your success rate will be. In the early stages, go heavy on the spawn, as you gain expertise, you can back off on the spawn. When your techniques get really good, you can grow mushrooms successfully with almost no spawn. For fruiting, Each strain responds to different triggering techniques to initiate fruiting. Some strains need a drop in temperature, some need a raise in temp, some will fruit without any triggering. They will almost all respond to drowning... take the well-colonized block and submerge it in water for 24-48 hrs to simulate drowning, and this will trigger fruiting. Wait at least 60 days after inoculation though, most new cultivators try to force fruiting too soon. That will just not work. Most strains fruit between 60-100 days. The best strain of Shiitake I have ever seen is the strain called 75 available from alohamedicinals.com It will fruit on just about any substrate, and does not need a triggering strategy. The wrong strain will be discouraging. In any species of mushroom cultivation, strain is important, but in shiitakes it is everything.

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Re: new to shiitake...please help from a pro [Re: JohnHolliday]
    #4765983 -

BTW, Coir is not a good additive for shiitakes and will limit the growth. Better to leave it out. Hardwood sawdust is the best, you can supplement with straw, and with cardboard but not too much. Banana leaves OK. We have yet to find a strain of shiitakes that will grow on coconut though, so skip the coir. Aslo, avoid conifer sawdust. Some shiitakes will fruit on it, but not many. Also Eucalyptus is OK for some strains, but for others it stops the growth. If that is your sawdust, try it and see how the growth is. Rosewood is a no-no. Ebony is also not good. What kind of wood do you have available?

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Re: new to shiitake...please help from a pro [Re: JohnHolliday]
    #4769381 -

hey thanks...you guys are great,this will save me some time and tears. the wood i have here is like teak or rubber trees. it's mostly redish and hard, I had seen others used them here in s.e. asia for oysters so i am hoping it will work for the shiitake. i was wondering doesn't bleach get weaker from the high heat in the pc?
peace
siam

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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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