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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Invisibleskyler
journeyman
Registered: 12/18/00
Posts: 73
dung troubles!!!
    #448728 - 11/06/01 07:21 AM (22 years, 10 months ago)

a friend is having trouble spawning dung with colonized grain. first he past. dung for 1.5-2hrs @ 160-180 deg.,
then lets drain for 24-48 hrs, then under sterile conditions mixes the two (grain/dung) in a container, covers with foil, and pokes holes..then lets sit to colonize ...... but, .he's been getting lots of contams before it 100% colonizes...should he even poke holes?? if he doesn't poke holes how will it get o2 ?? what about fanning? he hasn't been fanning should he?


please help.
thanx.

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OfflineAzure
old hand
Registered: 12/31/98
Posts: 469
Loc: California, USA
Last seen: 22 years, 5 months
Re: dung troubles!!! [Re: skyler]
    #448775 - 11/06/01 08:39 AM (22 years, 10 months ago)

Sucess is more likely if you sterilize gallon jars or fill up autoclavable bags with dung and innoculate with grain under sterile conditions.

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OfflinestonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666 666 Posts!
Last seen: 14 years, 6 months
Re: dung troubles!!! [Re: skyler]
    #448777 - 11/06/01 08:40 AM (22 years, 10 months ago)

This is the way i do it!
Pasturise 4# for 1? hrs...let drain for 5-6 hours..your supposed to barley squeese out some H20 from the dung..mix 1 pint of BRF-cakes 100% colonized + dung in a sterile bowl...put in a sterile plastic bag..poke some holes with
a fork..tie the bag up..Put in a paperbag, fold gently..Keep varm and dark..No fanning..Works great..
Peace
stonErollEr

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OfflineSuntzu
Geek
 User Gallery

Registered: 10/14/99
Posts: 1,396
Last seen: 2 months, 12 days
Re: dung troubles!!! [Re: stonErollEr1]
    #448830 - 11/06/01 09:58 AM (22 years, 10 months ago)

May also want to try LESS pasteurization, the best-colonizing dung I've ever seen also had a seedling or two that sprouted. Prepared as follows:
Pour boiling water over crispy-dry road apples, let hydrate for an afternoon or overnight. Done. Squeeze like you would a wet dishrag you're about to use to wipe off a countertop. Make sure the moisture content is good, or else the 'friendly flora' will not be so friendly.

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Invisibleskyler
journeyman
Registered: 12/18/00
Posts: 73
Re: dung troubles!!! [Re: Suntzu]
    #448883 - 11/06/01 10:45 AM (22 years, 10 months ago)

suntzu,
that's funny you say that because i just asked another question in the general forum about a blade of grass growing on a past. dung CASING....is this ok?? also, the casing had white long strands of something that appears to be cobweb mold...i don't see how when i past. for that long and was fanning the casing twice/day and noticed the strands after only 2 days after i cased....?
anyone have any suggestions?

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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