Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

MushroomMan Mycology
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Dirty cloning method with H2O2, seems to have worked.
    #4368638 -

I grew out some tiny shrooms. They dropped spores all over themselves. The stems were very thin so it would be difficult to open them up and get tissue that I was sure had no spores on it. I have no glove box or hood.

So I got 1/2 a stem and broke it up with my unwashed hands in open dirty air. I popped it all in a small glass jar with some broken glass and pure 3% H2O2. I shook it up and left for about 10mins. My idea was the spores would be killed and I could make a myc. tissue syringe. I sucked up 5cc of solution in a clean syringe through a self healing injection site and injected 2 jars.

2 days on, one of the jars shows growth in about 10 places, the other has what may be growth in 2.

If doing it again I would cut the stem up really fine with a razor or scissors.

I thought the strong peroxide may kill it. Does anybody know how long the tissue can survive in 3% peroxide? I still have some in it and will try again in a few weeks.

Extras: Filter Print Post Top
Re: Dirty cloning method with H2O2, seems to have worked. [Re: blackout]
    #4368641 -

H2O2 won't just destroy contams like a miracle cure, it only helps prevent contamination by a small percentage. I don't even use it anymore.

If your jars don't contam you are incredibly lucky or just really clean.

Extras: Filter Print Post Top
Re: Dirty cloning method with H2O2, seems to have worked. [Re: kronnyQ]
    #4368676 -

I know its not a miracle cure, but it is meant to kill all spores. I have heard of people working with peroxidated agar in open air with success. Its a very easy option if it does work. I put some in an LC but it shows no signs of growth yet. I thought the LC would have started quicker.

Extras: Filter Print Post Top
Re: Dirty cloning method with H2O2, seems to have worked. [Re: blackout]
    #4369031 -

i was reading that some had found that the initial dunk only proved as counterproductive to the growth of the culture as long as you were transferring to peroxidated media anyway.

i know for a fact that tissue will survive for weeks in peroxide.

it will take much longer to become acclimated to the medium, though.
:zoom:


--------------------



Extras: Filter Print Post Top
Re: Dirty cloning method with H2O2, seems to have worked. [Re: psilocyben]
    #4369097 -

I thought the LC would take off quicker since I injected only about 2cc in a jar of about 500ml 2% malt extract. I figured it would dilute the H2O2 so its effects would be minimal.
How strong was the peroxide in which your tissue lasted weeks? I think I should leave it in the sunlight to degrade faster to H2O

Extras: Filter Print Post Top
Re: Dirty cloning method with H2O2, seems to have worked. [Re: blackout]
    #4369156 -

Ya weeks would be a misleading statement since the peroxide would have been broken down in a matter of days after introducing foreign matter. Whenever I have done tissue culture transfers I have always done a 20 min. peroxide (3%) dunk. It's great insurance IME as well as many others I know that use the same procedure. GL


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* H2O2 - Hydrogen Peroxide SumGuy 2,255 4 08/30/01 03:06 PM
by BINDEGAL
* Peroxide in Liquid Tissue Culture? plaastikbob 1,773 5 01/30/02 03:29 AM
by Zen Peddler
* Measuring CO2 Level Che_Night_Soil 3,628 6 03/24/19 02:15 PM
by bodhisatta
* H2O2? wrestler_az 1,072 3 08/15/02 03:10 PM
by danelectro
* Peroxide Mist: Helpful or Harmful archibald 1,020 2 04/20/02 02:31 PM
by PinkFloyd
* h2o2? criminulmindid 877 1 11/25/01 02:47 PM
by Shdwstr
* peroxide % ?? growin 550 3 06/07/02 11:52 PM
by growin
* Mushroom cloning?? dimitri211 4,139 3 05/23/01 07:49 PM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
887 topic views. 4 members, 1,339 guests and 128 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.009 seconds on 14 queries.