Home | Community | Message Board


Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy Cannabis Seeds, Buy CBD   Amazon ½ Pint Jars, Agar, Malt Extract

Jump to first unread post. Pages: 1
Offlineblackout
Microwaver
 User Gallery

Registered: 07/17/00
Posts: 4,901
Last seen: 1 month, 21 days
Trusted Cultivator
Dirty cloning method with H2O2, seems to have worked.
    #4368638 - 07/04/05 05:35 AM (12 years, 10 months ago)

I grew out some tiny shrooms. They dropped spores all over themselves. The stems were very thin so it would be difficult to open them up and get tissue that I was sure had no spores on it. I have no glove box or hood.

So I got 1/2 a stem and broke it up with my unwashed hands in open dirty air. I popped it all in a small glass jar with some broken glass and pure 3% H2O2. I shook it up and left for about 10mins. My idea was the spores would be killed and I could make a myc. tissue syringe. I sucked up 5cc of solution in a clean syringe through a self healing injection site and injected 2 jars.

2 days on, one of the jars shows growth in about 10 places, the other has what may be growth in 2.

If doing it again I would cut the stem up really fine with a razor or scissors.

I thought the strong peroxide may kill it. Does anybody know how long the tissue can survive in 3% peroxide? I still have some in it and will try again in a few weeks.


--------------------
support the free spore ring http://fsre.nl/


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinekronnyQ
SuperstudExtraordinaire
 User Gallery

Registered: 07/23/04
Posts: 2,488
Loc: Anytown USA
Last seen: 1 year, 10 months
Re: Dirty cloning method with H2O2, seems to have worked. [Re: blackout]
    #4368641 - 07/04/05 05:39 AM (12 years, 10 months ago)

H2O2 won't just destroy contams like a miracle cure, it only helps prevent contamination by a small percentage. I don't even use it anymore.

If your jars don't contam you are incredibly lucky or just really clean.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineblackout
Microwaver
 User Gallery

Registered: 07/17/00
Posts: 4,901
Last seen: 1 month, 21 days
Trusted Cultivator
Re: Dirty cloning method with H2O2, seems to have worked. [Re: kronnyQ]
    #4368676 - 07/04/05 06:28 AM (12 years, 10 months ago)

I know its not a miracle cure, but it is meant to kill all spores. I have heard of people working with peroxidated agar in open air with success. Its a very easy option if it does work. I put some in an LC but it shows no signs of growth yet. I thought the LC would have started quicker.


--------------------
support the free spore ring http://fsre.nl/


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepsilocyben
bad guy

Registered: 12/03/04
Posts: 1,026
Last seen: 11 years, 4 months
Re: Dirty cloning method with H2O2, seems to have worked. [Re: blackout]
    #4369031 - 07/04/05 11:36 AM (12 years, 10 months ago)

i was reading that some had found that the initial dunk only proved as counterproductive to the growth of the culture as long as you were transferring to peroxidated media anyway.

i know for a fact that tissue will survive for weeks in peroxide.

it will take much longer to become acclimated to the medium, though.
:zoom:


--------------------




Post Extras: Print Post  Remind Me! Notify Moderator
Offlineblackout
Microwaver
 User Gallery

Registered: 07/17/00
Posts: 4,901
Last seen: 1 month, 21 days
Trusted Cultivator
Re: Dirty cloning method with H2O2, seems to have worked. [Re: psilocyben]
    #4369097 - 07/04/05 12:21 PM (12 years, 10 months ago)

I thought the LC would take off quicker since I injected only about 2cc in a jar of about 500ml 2% malt extract. I figured it would dilute the H2O2 so its effects would be minimal.
How strong was the peroxide in which your tissue lasted weeks? I think I should leave it in the sunlight to degrade faster to H2O


--------------------
support the free spore ring http://fsre.nl/


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 7 years, 8 days
Trusted Cultivator
Re: Dirty cloning method with H2O2, seems to have worked. [Re: blackout]
    #4369156 - 07/04/05 12:51 PM (12 years, 10 months ago)

Ya weeks would be a misleading statement since the peroxide would have been broken down in a matter of days after introducing foreign matter. Whenever I have done tissue culture transfers I have always done a 20 min. peroxide (3%) dunk. It's great insurance IME as well as many others I know that use the same procedure. GL


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy Cannabis Seeds, Buy CBD   Amazon ½ Pint Jars, Agar, Malt Extract

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* H2O2 - Hydrogen Peroxide SumGuy 1,169 4 08/30/01 05:06 PM
by BINDEGAL
* Peroxide in Liquid Tissue Culture? plaastikbob 1,102 5 01/30/02 05:29 AM
by Zen Peddler
* Measuring CO2 Level Che_Night_Soil 968 3 07/24/02 03:01 PM
by Che_Night_Soil
* H2O2? wrestler_az 564 3 08/15/02 05:10 PM
by danelectro
* Peroxide Mist: Helpful or Harmful archibald 764 2 04/20/02 04:31 PM
by PinkFloyd
* h2o2? criminulmindid 516 1 11/25/01 04:47 PM
by Shdwstr
* peroxide % ?? growin 444 3 06/08/02 01:52 AM
by growin
* Mushroom cloning?? dimitri211 2,505 3 05/23/01 09:49 PM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
677 topic views. 32 members, 139 guests and 22 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Crestline Sales - MycoPath
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.005 seconds on 19 queries.