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Amazon Shop for: Agar, HEPA Filter, Laminar Flow Hood, Paul Stamets

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Offline_blitzer
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Registered: 03/28/05
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Fake-ass Flowhood?
    #4356072 - 06/30/05 05:39 PM (11 years, 5 months ago)

  I read somewhere, sometime (on this message board), about a guy who made his ghetto flowhood with a rubbermaid container and an inexpensive Holmes HEPA filter from wally-world.  He claimed success.

How effective are the $20-$30 HEPA filters that can be bought at the world's leading merchandiser?  Are they effective enough to be used in the construction of a poorman's laminar flow hood?

  And to satisfy the people who would link me to a site on constructing a real one;  I don't have the money right now. :gonzo:


--------------------
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Offlinehyphae
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Re: Fake-ass Flowhood? [Re: _blitzer]
    #4356436 - 06/30/05 07:34 PM (11 years, 5 months ago)

A still air glovebox will give you the best results but if you must have a getto flowhood remember you'll be introducing large volumes of semi-sterile air so chances of contams go up compared to still air (FACT!) I have heard of many using these with good success so you will have to find out for yourself, IME it all depends on local and sporeloads :wink: GL and have fun :laugh:


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
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Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineWorkmanV
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Re: Fake-ass Flowhood? [Re: _blitzer]
    #4358921 - 07/01/05 11:31 AM (11 years, 5 months ago)

They are actually better than you might expect. I tested the Holmes model HAP240 by putting an agar plate in the air stream for 60 seconds with no contamination at all. It is not rated as high as a professional HEPA but it is adequate for a hobbiest.

I also found this setup online using the same unit for sterile rose tissue culture. It is pretty crude but gives an idea of what can be done. I am sure with some tweaking it could be better.

Fake-ass Flowhood


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Offlinepcubmycol
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Re: Fake-ass Flowhood? [Re: Workman]
    #4358971 - 07/01/05 11:47 AM (11 years, 5 months ago)

the are actually pretty good filters, my friend tommy uses one in his grow closet and has run casings til the 6th flush... he has only had one contam in over a year of casings... i would imagine you could make a ghetto flowhood pretty nicely with one.


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:-D


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InvisibleTien
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Re: Fake-ass Flowhood? [Re: pcubmycol]
    #4359017 - 07/01/05 12:05 PM (11 years, 5 months ago)

^^^^^^^
Sorry for going off topic,
I just wanted to say that I love reading "feeling rhizmorphic?"
lol


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OfflineHotnuts
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Re: Fake-ass Flowhood? [Re: Workman]
    #4359026 - 07/01/05 12:11 PM (11 years, 5 months ago)

Quote:

Workman said:
They are actually better than you might expect. I tested the Holmes model HAP240 by putting an agar plate in the air stream for 60 seconds with no contamination at all. It is not rated as high as a professional HEPA but it is adequate for a hobbiest.

I also found this setup online using the same unit for sterile rose tissue culture. It is pretty crude but gives an idea of what can be done. I am sure with some tweaking it could be better.

Fake-ass Flowhood




Here's my rig that i've got about 45-50 bucks into. The Holmes filters are fine IMO. I've never had a problem with mine at all, and the area I live in is pretty thick with molds at this time of the year. Nothin fancy, but works like a charm.


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Offlinepcubmycol
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Re: Fake-ass Flowhood? [Re: Tien]
    #4359027 - 07/01/05 12:11 PM (11 years, 5 months ago)

Quote:

Plutonium said:
^^^^^^^
Sorry for going off topic,
I just wanted to say that I love reading "feeling rhizmorphic?"
lol




I established a long time ago when i first got into this hobby that that just seems like a good way to feel... I try to feel it everyday!

Happy trails, shrooming, and "fake-ass flowhood" making... :thumbup:


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Offline_blitzer
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Re: Fake-ass Flowhood? [Re: _blitzer]
    #4359326 - 07/01/05 01:46 PM (11 years, 5 months ago)


Hey thanks to alla'yall that responded with the info. (Which state am I from bitch?!)

I'm off to wally world to make my fake ass flowhood!


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OfflineGamera
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Re: Fake-ass Flowhood? [Re: hyphae]
    #4359373 - 07/01/05 01:59 PM (11 years, 5 months ago)

Quote:

hyphae said:
A still air glovebox will give you the best results but if you must have a getto flowhood remember you'll be introducing large volumes of semi-sterile air so chances of contams go up compared to still air (FACT!) I have heard of many using these with good success so you will have to find out for yourself, IME it all depends on local and sporeloads :wink: GL and have fun :laugh:




I have been wondering about this.  I keep seeing glove boxes with fans on them (sometimes described as "positive pressure glove box") but to my mind you are adding contaminants even with a HEPA filter on it (though very few perhaps) as opposed to just closing it up as best you can and making sure you clean the snot out of it before you get started.  The principal behind a flow hood is that you get a laminar flow so no contaminants will settle, and inside a glove box whatever containants you do have are just getting swirled around if you have a fan going.  It would seem that a fan would just make matters worse.  Sure, it keeps conatiminants from falling in, but with a typical container this seems to me less likely than contaminants getting in through a filter.

Am I off base on this?


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Money is human happiness in the abstract; he, then, who is no longer capable of enjoying human happiness in the concrete devotes himself utterly to money.

Arthur Schopenhauer


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InvisibleRoadkill
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Re: Fake-ass Flowhood? [Re: Gamera]
    #4359388 - 07/01/05 02:02 PM (11 years, 5 months ago)

I use a still air glovebox.

haven't had a contam in a long long time.

:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinepcubmycol
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Re: Fake-ass Flowhood? [Re: Gamera]
    #4359414 - 07/01/05 02:06 PM (11 years, 5 months ago)

I think your right on here... a still air glovebox seems to be much more sterile IMO.


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weretheshit.com is the shit!

:-D


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Offline_blitzer
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Re: Fake-ass Flowhood? [Re: Roadkill]
    #4359442 - 07/01/05 02:13 PM (11 years, 5 months ago)

Quote:

Roadkill said:
I use a still air glovebox.

haven't had a contam in a long long time.

:smile:




pic's RK?


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InvisibleRoadkill
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Re: Fake-ass Flowhood? [Re: _blitzer]
    #4359452 - 07/01/05 02:14 PM (11 years, 5 months ago)

hang on...

I'll go take some.

:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offline_blitzer
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Re: Fake-ass Flowhood? [Re: _blitzer]
    #4359502 - 07/01/05 02:25 PM (11 years, 5 months ago)

For some reason do not find myself attracted to a still air glovebox, never liked the idea since I 1st read about it in TMC. I think it is the lack of workspace, the confinement of a box...
Having to load it, lysol it, and then worry about my hands, or even the holes I put them through, being a vector for contams. And I certainly can't fit my canner in there.

Just seems like even the most basic flowhood setup would be more efficient and easire to apply to the hobby.

I think I'm going to add an apropriatly sized high-quality A/C filter (made by Arm&Hammer? seen them, orange packaging can't remember the name grrrr.....) to the back of the fake flowhood pictured, 2"-3" from the filter output to act as a plenum; that should more evenly distribute the airflow, and filter it again.



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Offlinepcubmycol
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Re: Fake-ass Flowhood? [Re: _blitzer]
    #4359517 - 07/01/05 02:29 PM (11 years, 5 months ago)

That will be just that much more sterile, but ultimately, i dont think you could beat a still air glovebox unless you wanted to spend tons of $. making a completely sterile room.


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weretheshit.com is the shit!

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OfflineIGnosticAbhorI
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Re: Fake-ass Flowhood? [Re: pcubmycol]
    #4359595 - 07/01/05 02:51 PM (11 years, 5 months ago)

Quote:

unless you wanted to spend tons of $. making a completely sterile room.




You can make a ghetto sterile room for cheap...:smirk:

But if you mean a Professional sterile room...then yea..big $

-Gnostic


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Offline_blitzer
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Re: Fake-ass Flowhood? [Re: IGnosticAbhorI]
    #4380137 - 07/07/05 04:36 PM (11 years, 5 months ago)

<-- missed out on RK's glovebox pic's


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Amazon Shop for: Agar, HEPA Filter, Laminar Flow Hood, Paul Stamets

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