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Invisibleohmatic
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spores on agar - the first time
    #4198344 - 05/20/05 05:20 AM (12 years, 1 month ago)

ey!

so i had the opportunity to use a mates flowhood for transferring spore to agar,
i personally just did peroxided agar for cloning so far, so this was something new for me.

anyways, id like to ask about a certain phenomenon which i also recognised when
dealing with tissue on agar.

namely, the area where the spores (or the tissue..) starts to grow on the agar
first is being concentrically colonised with a sort of 'translucent mycelium'

check these couple pics for what im talking about:



you can see the fine fibers when you hold it agains light and check the reflection,
but yet it isnt white.

usually this circle grows till about 0.2inches in diameter, then starts
to turn to white, healthy mycelium from the beginning and growing all the way.

i wonder what this kind of "translucent" mycelium is that appears before the
"white" mycelium starts to grow, as i know its NO contam or anything,
its just funky :wink:

for all the people with more spore on agar experience, share it wit lil ohm pllls :sun:

btw, both dishes were a week old when the pic was taken.
peace ohm :mushroom2:

THESE PICTURES WERE TAKEN HOLDING THE DISHES IN FRONT OF A LAMP
TO BE ABLE TO SHOW THESE 'TRANSLUCENT' STRANDS.
CHECK BELOW FOR SHOTS IN SUNLIGHT THAT LOOK COMPLETELY DIFFERENT


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Edited by ohmatic (05/23/05 04:46 AM)


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Offlineshirley knott
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Re: spores on agar - the first time [Re: ohmatic]
    #4198374 - 05/20/05 05:48 AM (12 years, 1 month ago)

ummm.... :frown:  that's not mycelium i'm afraid.
it's some kinda contaminant bacteria.


--------------------
buh


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Invisibleohmatic
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Re: spores on agar - the first time [Re: shirley knott]
    #4198377 - 05/20/05 05:51 AM (12 years, 1 month ago)

the deal ism it all starts to get white from the center now!

i know the pics sorta look strange but in nature it really looks good,
damn im seriously confused now you know :wink:
peace ohm :mushroom2:


--------------------
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RIP #cultivation! ....can't associate? well FUCK U !


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OfflineAnand
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Re: spores on agar - the first time [Re: shirley knott]
    #4198379 - 05/20/05 05:52 AM (12 years, 1 month ago)

Quote:

shirley knott said:
ummm.... :frown:  that's not mycelium i'm afraid.
it's some kinda contaminant bacteria.




don't be too sure... i have seen myc from spores start that way on agar


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Invisibleohmatic
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Re: spores on agar - the first time [Re: Anand]
    #4198411 - 05/20/05 07:39 AM (12 years, 1 month ago)

Quote:

Anand said:
Quote:

shirley knott said:
ummm.... :frown:  that's not mycelium i'm afraid.
it's some kinda contaminant bacteria.




don't be too sure... i have seen myc from spores start that way on agar




and the funky thing is, it IS starting to turn white :wink:
for some reason i cannot edit my initial post b/c of some database error
but as soon as its up and running again ill add daylight pics which are WAY better :laugh:
peace ohm :mushroom2:

*edit*
in the meantime
*edit*
SAME petris as above, but this time in daylight, note the difference in looks!



--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Edited by ohmatic (05/20/05 07:41 AM)


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OfflineEightball
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Re: spores on agar - the first time [Re: ohmatic]
    #4198698 - 05/20/05 10:42 AM (12 years, 1 month ago)

my pink oysters (from mycelium syringe) pulled some bacterial contam looking crap similar to yours for close to a week then there was fine mycelium running from the splotches and all was well.


--------------------
If you're frightened of dying and you're holding on.you'll see devils tearing your life away.
But...if you've made your peace, then the devils are really angels
Freeing you from the earth.


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Re: spores on agar - the first time [Re: Eightball]
    #4198740 - 05/20/05 10:52 AM (12 years, 1 month ago)

thanks for the info guys! this gives me hopes on my hericium colloidies spore plates! :smile:


Edited by mattymonkey (05/20/05 11:04 AM)


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Offlineshirley knott
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Re: spores on agar - the first time [Re: ohmatic]
    #4198779 - 05/20/05 11:01 AM (12 years, 1 month ago)

blimey - those daylight shots look completely different and much more promising. I hope i was wrong - get a tiny white bit transferred to a peroxidated plate asap!

this was one of my first ever agar plates: -cyan by name and cyan by nature :frown:


--------------------
buh


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Re: spores on agar - the first time [Re: shirley knott]
    #4198839 - 05/20/05 11:18 AM (12 years, 1 month ago)

Quote:

shirley knott said:
blimey - those daylight shots look completely different and much more promising. I hope i was wrong - get a tiny white bit transferred to a peroxidated plate asap!

this was one of my first ever agar plates: -cyan by name and cyan by nature :frown:




why not wait till i got properly established mycelium on the dishes?
like having them 3/4th colonised or so?
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Re: spores on agar - the first time [Re: ohmatic]
    #4206042 - 05/22/05 10:43 AM (12 years, 1 month ago)

update on plates 2 days later,
theres quite some action going on guys :sun:





so, besides the pfclassic plate II, they all show nice signs of growth and its
soon going to be time to transfer some pieces of them to peroxided agar.

i would LOVE suggestions of what parts to transfer, as its all like uhm,
pretty cottony anyway (no rhizos to my eye).

so if someone want to draw a little circe/square or something on sectors looking good,
this would be greatly appreciated !

also any comments/input is greatly appreciated.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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InvisibleRoadkill
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Re: spores on agar - the first time [Re: ohmatic]
    #4206125 - 05/22/05 11:23 AM (12 years, 1 month ago)

hi..bro

I got your pm...and took a look at your work.

Nice work!~

In this 1st picture you can see the start of some agressive growth.


I have marked in red what to part to watch.
then transfer from these area's when it has grown out a little more...to new plates.
you might have to do several transfers before you get some really aggresive mycelium.

tc


--------------------
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Re: spores on agar - the first time [Re: Roadkill]
    #4206164 - 05/22/05 11:51 AM (12 years, 1 month ago)

thx very much roadkill, actually i didnt even see that section up to now :wink:

ill watch it closely!

oh and b/c i didnt do it yet .. +5 :laugh:
peace ohm :mushroom2:

*edit*
i just found out i already rated you heh,
oh well, think of it as an imaginary 2nd +5 then :heart:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Edited by ohmatic (05/22/05 11:52 AM)


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Re: spores on agar - the first time [Re: ohmatic]
    #4206554 - 05/22/05 02:19 PM (12 years, 1 month ago)

I'm realy new to everything but PFTEK. Where can I buy agar? or is it agar agar like some of the TEKs say? Is it the same as gelatin? Then I mix with dextrin or malt extract? Same thing with peroxide to get an isolated culture?


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Re: spores on agar - the first time [Re: drugsaregood]
    #4206596 - 05/22/05 02:27 PM (12 years, 1 month ago)

Quote:

drugsaregood said:
I'm realy new to everything but PFTEK.  Where can I buy agar?  or is it agar agar like some of the TEKs say?  Is it the same as gelatin?  Then I mix with dextrin or malt extract?  Same thing with peroxide to get an isolated culture?




http://en.wikipedia.org/wiki/Agar

get it @ lab supply companies or some chinese stores.
its the same as agar agar and hasnt got anything in common as gelatine.
read here for agar recipies: http://www.shroomery.org/forums/showflat...9bc589c12051784

read here for peroxide agar tek: http://www.shroomery.org/forums/showflat.php/Cat/0/Number/466150/an/0/page/0
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Re: spores on agar - the first time [Re: ohmatic]
    #4206753 - 05/22/05 03:10 PM (12 years, 1 month ago)

your streaking pattern sucks, just put spores in the center of the dish and that way you can pick an section to isolate when it starts racing across the plate.
Here are some wood lovers. I am concerned that the top left dish might be a forign mycelium. The top right is good to go, typical ps. mycelium. The bottom is a contaminated transfer, note the mycelium is growing faster then the mold.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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Re: spores on agar - the first time [Re: tahoe]
    #4206777 - 05/22/05 03:18 PM (12 years, 1 month ago)

well, actually i did the streaking being advised by an old pro,
i guess the zig zag pattern used isnt thaaaaaat bad after all :wink:

anyway, i can clearly see your point with the spores in the middle making
it a lot easier to isolate nicely aggressive strains.

i think the next time i will do it like that,
but for now, i will have to work with what i got and being honest,
i like the way the mycelium is developing so far.

anyway, someone, besides roadkill, got some more pointers for transfer areas?
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Re: spores on agar - the first time [Re: ohmatic]
    #4207128 - 05/22/05 05:40 PM (12 years, 1 month ago)

great thread.. fun to watch the myc spread! good luck on your isolate ohm...


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Re: spores on agar - the first time [Re: ohmatic]
    #4225005 - 05/27/05 02:20 AM (12 years, 1 month ago)

so i did a couple of transfers yesterday,
updates will come in a couple of days when growth has reached the agar.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Re: spores on agar - the first time [Re: ohmatic]
    #4225042 - 05/27/05 02:35 AM (12 years, 1 month ago)

i love doing transfers. look at the rhizos on the lower half of this one. It was transfered from a chunk of non rhizomorphic mycelium


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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Re: spores on agar - the first time [Re: tahoe]
    #4225058 - 05/27/05 02:39 AM (12 years, 1 month ago)

the problem i encountered when doing the transfers was
that i didnt really have perfectly rhizo myc yet.

i transferred parts that looked the least cottony and decided
to do about 2 to 3 plates of each master plate, using different
areas in order to increase chance of getting a nice rhizo in one or
two additional isolates.

what is going to be VERY itneresting is, that once i cloned a fruit
and isolated a rhizo stem of it, i will seperatedly grow out the cloned
fruit and the isolated multispore rhizo stem, i am really curious about the
results :smile:

but so far that will be some more waiting hehe.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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