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Invisiblemycofile
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Registered: 01/19/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: Does agar germination look pinkish?
    #90492 - 11/18/99 12:16 AM (17 years, 6 months ago)

Pink is a contam. I'll look up which one in TMC if you want. It may give some insight as to where it came from. A soon as you get mycelium growth not directly beside the pink, do a transfer of the mycelium to fresh agar. Peroxide agar would work well. Placebo did a good write up on peroxide agar about a month ago in the old cultivation forum, do a search for it. It sucks, but is the way of life of an agar cultivar.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Anonymous

Re: Does agar germination look pinkish?
    #90494 - 11/18/99 12:27 AM (17 years, 6 months ago)

Pink contam is all over every single plate!!

Please tell me from the TMC a probbl cause..



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Anonymous

Re: Does agar germination look pinkish?
    #90495 - 11/18/99 12:51 AM (17 years, 6 months ago)

I have had this problem befour and it is a major pain in the ass. The next time you make up some agar/dishes, wait a few days befour you transfer your spores to it. At least you won't waste spores. good luck.


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OfflineCFH(COWBOY FROM HELL)
newbie
Registered: 05/07/99
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Last seen: 16 years, 1 month
Re: Does agar germination look pinkish?
    #90496 - 11/18/99 11:09 PM (17 years, 6 months ago)

I have some eqs on agar and the mycelium is very fluffy and white .Sorry to hear about the contaminates.


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Invisiblemycofile
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Registered: 01/19/99
Posts: 2,336
Loc: Uranus
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Re: Does agar germination look pinkish?
    #90497 - 11/20/99 05:59 PM (17 years, 6 months ago)

Is the contaminant mycelia fast growing and aerial, or slow growing and appressed?
If the first, your looking at a Neurospora, or a Trichothecium for the second. Choose one, and I'll give more specifics.
I love TMC!
I hate contams

Also, where did the contam first show up, near the point of innoculation, spread evenly across the plate, or near the peripherry?
------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)

[This message has been edited by mycofile (edited November 20, 1999).]



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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OfflineB.I.O.
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Registered: 05/27/99
Posts: 455
Last seen: 5 years, 6 months
Re: Does agar germination look pinkish?
    #90498 - 11/21/99 04:59 AM (17 years, 6 months ago)

we now this pink bacterium too...wether your sterilisation is poor, or tools or spores are aready carrying the bact.

BiO.



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Invisiblemycofile
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Registered: 01/19/99
Posts: 2,336
Loc: Uranus
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Re: Does agar germination look pinkish?
    #90499 - 11/22/99 03:44 AM (17 years, 6 months ago)

Exactly BIO, I too have seen both types of "pink mold". The Neurospora seems to have been contaminating a print I had, as the contaminant germation always grew wherever the spores were. The Trichothecium seems to have been due to either sterilization, or improper sterile technique, as it was in various places in the plates. Luckily I only had these in 1 batch a piece.

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Anonymous

Re: Does agar germination look pinkish?
    #90500 - 11/23/99 04:55 AM (17 years, 6 months ago)

I cannot get rid of the pink stuff which after all just appears makes a pink blob and doesn't grow.

Its just there....

I now know this "I probly shouldn't have used spore WATER"

But please if anyone can elaborate on it....



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OfflineBenzl
enthusiast
Registered: 01/06/99
Posts: 122
Last seen: 2 years, 3 months
Re: Does agar germination look pinkish?
    #90501 - 11/24/99 09:00 PM (17 years, 6 months ago)

The Agar does seem to be prone to the red jelly mould, I reckon that it is the most poisonous thing that I have seen yet and never got much colonisation past its appearance so that one is probably a bin job.


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OfflineThunderfuck
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Registered: 07/20/99
Posts: 356
Loc: Quebec, Canada
Last seen: 12 years, 11 months
Re: Does agar germination look pinkish?
    #90503 - 11/25/99 12:22 PM (17 years, 6 months ago)

Same here, Moe. I'm clueless...

------------------
We're just two lost souls
Swimming in a fish bowl,
Year after year,
Running over the same old ground.
What have we found?
The same old fears.
Wish you were here.



--------------------
now is the only thing that's real


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Invisiblemycofile
Pooh-Bah
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Registered: 01/19/99
Posts: 2,336
Loc: Uranus
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Re: Does agar germination look pinkish?
    #90504 - 11/29/99 12:30 PM (17 years, 5 months ago)

Well, I just saw a plantasia agar jar overcome this shit! I set the jar in the throw out pile, and just didn't get to it. 2 weeks later, the whole plate (including the invading contam) was covered in plantasia mycelia! Still throwing it out, but tells you something.....what I'm not sure, maybe just an unrelated storry, but that's something :wink:

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Does agar germination look pinkish?
    #90505 - 12/23/99 09:55 AM (17 years, 5 months ago)

Hmmmmm........


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Invisibleanandam
skrat

Registered: 10/29/99
Posts: 22
Re: Does agar germination look pinkish?
    #90506 - 12/24/99 02:57 AM (17 years, 5 months ago)

We are from all over the planet and got the same contam.at same time.Mother earth give us a opportunity to know more about competitors and win the battle.Good luck.


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Amazon Shop: ½ Pint Jars, Agar, Paul Stamets

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