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Amazon Shop: ½ Pint Jars, Agar, Autoclave

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InvisiblelIllIIIllIlIIlIlIIllIllIIl
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extending and improving shelf-life of prints
    #3902829 - 03/11/05 02:35 PM (12 years, 2 months ago)

I think it can be agreed that over time spore prints lose some vigor and become increasingly difficult to grow. Keeping them in a dark, dry place seems to extend their lifetime to five years or so. That suggests moisture and UV light kills them.

Is it possible that oxygen also affects the spores? Even if a spore is kept dry and in a dark place, it will still oxidize.

Because dry ice is so amazingly cheap (less than two dollars a pound) it would be very easy to place spore prints in an airtight container containing only CO2, and almost no O2.

I am willing to experiment with this idea, and would like your feedback. Do you think spores are damaged by oxygen? Do you think sealing them away in CO2 could keep them fresh and unchanged over many years?

Thanks for reading this; look forward to your responses.


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OfflineEdge
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Re: extending and improving shelf-life of prints [Re: lIllIIIllIlIIlIlIIllIllIIl]
    #3902867 - 03/11/05 02:41 PM (12 years, 2 months ago)

well my friend, 5 years is a pretty damn long shelf life but all the power to ya if you find a way to increase it!

to answer your question though, i dont know. ive never even considered increasing shelf life. let us know how your results are then, im interested.


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InvisibleRoadkill
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Re: extending and improving shelf-life of prints [Re: lIllIIIllIlIIlIlIIllIllIIl]
    #3903337 - 03/11/05 04:12 PM (12 years, 2 months ago)

Quote:

Is it possible that oxygen also affects the spores? Even if a spore is kept dry and in a dark place, it will still oxidize.




Possibly

I'd just try vaccum sealing them if you want to try for a much longer shelf life than 5 years.

I have some prints that are nearing the 5 year mark...they are kept in a plastic file box...in a dark, cool place.
I will be checking the viability of them in the next year...and following up on them in a semi annual basis.

good luck!~


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineIGnosticAbhorI
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Re: extending and improving shelf-life of prints [Re: Roadkill]
    #3903366 - 03/11/05 04:17 PM (12 years, 2 months ago)

Yea, I think that's what Agar does, Puts them in vials, Vaccum sealed? And has them stored away, suppost to last a really long time, he had a nice post about it :thumbup:


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Invisibleagar
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Re: extending and improving shelf-life of prints [Re: IGnosticAbhorI]
    #3903430 - 03/11/05 04:27 PM (12 years, 2 months ago)

Print on autoclave bag material & vaccum - double impulse seal.  :thumbup:


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InvisibleRoadkill
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Re: extending and improving shelf-life of prints [Re: IGnosticAbhorI]
    #3903434 - 03/11/05 04:28 PM (12 years, 2 months ago)

you might be thinking about how agar stores his mycelium in sterile water.

that will last over a decade.

I do that too.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineIGnosticAbhorI
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Re: extending and improving shelf-life of prints [Re: agar]
    #3903436 - 03/11/05 04:28 PM (12 years, 2 months ago)

^^ lol :thumbup:

-Gnostic


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InvisibleRoadkill
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Re: extending and improving shelf-life of prints [Re: agar]
    #3903457 - 03/11/05 04:30 PM (12 years, 2 months ago)

Quote:

agar said:

Print on autoclave bag material & vaccum - double impulse seal.  :thumbup:




Speaking of the devil.  :smirk:

Hope you are feeling better bro!!!!

I just back from out of town...the dial up connection really sucked at the gal pals house.
Glad to be home...and have my tweaked cable back.
:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisiblelIllIIIllIlIIlIlIIllIllIIl
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Re: extending and improving shelf-life of prints [Re: Roadkill]
    #3903550 - 03/11/05 04:47 PM (12 years, 2 months ago)

That vacuum bag idea sounds good. I might try that eventually when I can scrape enough cash together. In the mean time though I have quite a few 125ml canning jars and as I said dry ice is very inexpensive.

I guess the main prob is waiting that long, ten years or so and then making a syringe.


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InvisibleTODAY
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Re: extending and improving shelf-life of prints [Re: lIllIIIllIlIIlIlIIllIllIIl]
    #3903561 - 03/11/05 04:51 PM (12 years, 2 months ago)

dry ice sublimates too. you need a damn cold freezer to keep that shit for long.


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InvisibleHolydiver
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Re: extending and improving shelf-life of prints [Re: TODAY]
    #3903580 - 03/11/05 04:56 PM (12 years, 2 months ago)

Vinegar + baking soda is a cheap method of making your own C02 if you have any lying around.


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To find a place to live between the negatives and positives.


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Amazon Shop: ½ Pint Jars, Agar, Autoclave

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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