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Offlineadt
newbie
Registered: 06/03/01
Posts: 46
Loc: west coast USA
Last seen: 15 years, 5 months
spores from dried caps...
    #336246 - 06/06/01 04:25 PM (15 years, 9 months ago)


i posted this question a couple of days ago, and i got some good ideas, and now ive got some kind of plan, which id like to get some feedback on at this point:

i have plenty of dried, closed caps (i figure closed cap has a MUCH smaller chance of contamination). i would like to (in a sterile setting), cut open a cap, remove some gill material with sterile tweezers and blade, break it up into dust, and soak this in sterile water for a new syringe or two. i know this may not be ideal, but i live in CA, and it doesnt seem that easy to get spores here. like i said, i have PLENTY of dried specimens to use for this. will this work?

someone also suggested i inject sterile water into a closed cap, but id think that the tek above would get more spores from the dried gills.

someone else suggested using agar to culture a dried gill, and pick a winner from that to use for spores. i like the sound of this, yet my patience would prefer a more direct approach.

ANYBODY with any experience using dried caps for spores please share your thoughts. does anyone think my plan at the top would give ok to good results? let me know, & ill love you forever.

take it ez, respect,
aaron




--------------------
"Heaven is in a cow's ass." -BH


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Offlineegghead
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Registered: 04/25/01
Posts: 1,054
Loc: Milky Way
Last seen: 15 years, 3 days
Re: spores from dried caps... [Re: adt]
    #336346 - 06/06/01 06:37 PM (15 years, 9 months ago)

Sounds like agar is your best bet for success.



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InvisibleBrownPastures
old hand

Registered: 03/16/01
Posts: 968
Loc: here
Re: spores from dried caps... [Re: egghead]
    #336354 - 06/06/01 06:43 PM (15 years, 9 months ago)

Hey egghead ... when's that last time you grew anything from DRIED samples??? i think you just missed that part;).... closed caps have immature spores... peace

"like a Japanese Cowboy or a blind man on skates"-Ween
Drool Donkey Island


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Offlineadt
newbie
Registered: 06/03/01
Posts: 46
Loc: west coast USA
Last seen: 15 years, 5 months
Re: spores from dried caps... [Re: BrownPastures]
    #336382 - 06/06/01 07:07 PM (15 years, 9 months ago)


so am i screwed then? a few people have told me, in different ways, that i COULD use dried caps to get spores from. (i have closed & open, in abundence)

any suggestions here would be great...

respect,
aaron



--------------------
"Heaven is in a cow's ass." -BH


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InvisibleBrownPastures
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Re: spores from dried caps... [Re: adt]
    #336390 - 06/06/01 07:11 PM (15 years, 9 months ago)

ok ... here you go...

If your going to try your had at innoculation from these fruits your going to need 99% luck and the other 1% is tek..
basicly what you want to do is ok ... but i wouldn't suggest throwing in crumbled up gills... see if you can tap some spores off of the mature fruits.. if not then , as you said, your screwed... peace and good luck ....

"like a Japanese Cowboy or a blind man on skates"-Ween
Drool Donkey Island


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OfflineAnnoA
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Re: spores from dried caps... [Re: BrownPastures]
    #336418 - 06/06/01 07:50 PM (15 years, 9 months ago)

.... closed caps have immature spores...
Not necessarily.
Some(most?) strains begin to drop spores shorly before the cap opens.
So placing a gill on agar would still be a possible successful move.

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InvisibleBrownPastures
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Re: spores from dried caps... [Re: Anno]
    #336464 - 06/06/01 09:06 PM (15 years, 9 months ago)

<yawn>

"like a Japanese Cowboy or a blind man on skates"-Ween
Drool Donkey Island

Edited by BrownPastures on 06/06/01 09:07 PM.



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OfflineAnnoA
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Re: spores from dried caps... [Re: BrownPastures]
    #336475 - 06/06/01 09:20 PM (15 years, 9 months ago)

Tired? Take a nap.

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InvisibleBrownPastures
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Re: spores from dried caps... [Re: Anno]
    #336488 - 06/06/01 09:33 PM (15 years, 9 months ago)

"Some(most?) strains begin to drop spores shorly before the cap opens. "

Isn't that a mature fruit or are you just a smart-ass???

"buh-bye"SNL David Spade

"like a Japanese Cowboy or a blind man on skates"-Ween
Drool Donkey Island


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OfflineAnnoA
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Re: spores from dried caps... [Re: BrownPastures]
    #336493 - 06/06/01 09:40 PM (15 years, 9 months ago)

Sure it is.
have plenty of dried, closed caps
Does this sentence imply the fruits aren?t mature?
I?m not sure who is being a smart-ass...

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InvisibleBrownPastures
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Registered: 03/16/01
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Re: spores from dried caps... [Re: Anno]
    #336505 - 06/06/01 09:56 PM (15 years, 9 months ago)

yes i would think "closed caps" means immature fruits... a cap that is showing it's veil attached to the rim and the stem obviously starts sporulation because it's about to start doing it. it doesnt' necissarily mean that the cap is closed...i think it's just that you dont like me that your picking apart this poor souls post...

"like a Japanese Cowboy or a blind man on skates"-Ween
Drool Donkey Island


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OfflineAnnoA
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Re: spores from dried caps... [Re: BrownPastures]
    #336510 - 06/06/01 10:03 PM (15 years, 9 months ago)

OK, I think enough?s been said.
And, BrownPastures, I neither like nor dislike you, I just try to give "this poor soul" some advice as good as I can.


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InvisibleBrownPastures
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Re: spores from dried caps... [Re: Anno]
    #336520 - 06/06/01 10:19 PM (15 years, 9 months ago)

I agree. I'm glad i haven't ruffled any feathers. Peace

"like a Japanese Cowboy or a blind man on skates"-Ween
Drool Donkey Island


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