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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Zero growth after 2 days
    #29615212 -

Hello, I inoculated some agar plates for the first time on 6/29 with a spore syringe of b+ and have seen zero growth so far storing at roughly 70 degrees in the closet, also inoculated plates with a penis envy spore isolate on 6/30 and have zero growth on that also, I'm wondering if i did something wrong, I used milkboys agar recipe and they came out looking super clean.

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Re: Zero growth after 2 days [Re: Mortifiied]
    #29615230 -

Patience is key.

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Re: Zero growth after 2 days [Re: Fullcircle1] * 1
    #29615297 -

:whathesaid: Sometimes spores take a week or two to germinate, 4 or 5 days on the fast side. Nothing to worry about :cool::mushroom2:


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Pasteurizing Straw with Hydrated Lime
Swiss Cheese Fruiting Chamber

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Re: Zero growth after 2 days [Re: Agar Wedge] * 2
    #29615389 -

:whathesaid: (they)

2 days is nothing, if you see anything grow in 2 days from spores it's probably gonna be mold so the fact that you've got nothing on there now is a good thing tbh. I usually get the first growth around day 4-10 so just relax and wait.

Good job with the agar though :rockon:


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Re: Zero growth after 2 days [Re: SkanskKorv]
    #29615473 -

PE definitely looked like a LC but it said spore isolate on syringe b+ was very clearly just spores

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Re: Zero growth after 2 days [Re: Mortifiied]
    #29617084 -

Update still zero growth on the rye berrie jars and plates all still super clean.

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Re: Zero growth after 2 days [Re: Mortifiied]
    #29617112 -

Monday there´ll be some growth :wink:

    :cookiemonster:


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Re: Zero growth after 2 days [Re: Goatrider]
    #29617566 -

Im praying:prayer:

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Re: Zero growth after 2 days [Re: Mortifiied] * 1
    #29617644 -

Mugsey429 said:
Update still zero growth on the rye berrie jars and plates all still super clean.


You know I'll acknowledge and praise you for testing out your LC. I'm not sure why you injected into your grain at the same time. The idea behind the test is to not waste grain.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

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Re: Zero growth after 2 days [Re: DV8DUG] * 1
    #29618044 -

It wasn't LC it said spore isolate on the syringe and kinda looked like LC but I was wrong, The only reason I went ahead and put some in 4 jars is because I wanted some shrooms as soon as possible lol, and if it works out great, I already have all the supplies to do my own jars of oats those were 4-12 pre sterilized i bought from outgrow just to get jumpstarted back in to growing. I plan on doing like 4 18 oz monos and 2-4 66qt at the same time. Everything else is getting inoculated from agar though, and maybe some LC i make from the agar.
Also WE HAVE GROWTH BOYS!! Not in the grain as far as i can see but the agar, pics soon..

Edited by Mortifiied (07/05/26 02:44 PM)

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Re: Zero growth after 2 days [Re: Mortifiied]
    #29618116 -

Just for info- if posting pics of plates a uniform background helps a lot. Grey/ black, or if you want it back illuminated just white paper with a light source behind.


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Re: Zero growth after 2 days [Re: bluescreen]
    #29619454 -















Update pics on the plates let me know what you all think, theirs a few with this wispy looking growth not sure if its mold or just myc.
Also those 2 stacks of plates didn't get any growth wondering if I should just trash them?
Any tips on next steps would be greatly appreciated.

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Re: Zero growth after 2 days [Re: Mortifiied]
    #29619471 -

Let them grow out some more, time them. If it is mold it will haste and fill out your plates in no time.


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Zero growth after 2 days [Re: Kloakk] * 1
    #29619476 -

What he said :rockon:


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An enigma some caerulescens n a mono 😎

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Re: Zero growth after 2 days [Re: Kloakk]
    #29619550 -

Ok thanks everyone, Updates in a few days.

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Re: Zero growth after 2 days [Re: Mortifiied]
    #29619552 -



In regards to a growth like the one on the left what are we thinking?
Theirs another b+ plate with just 1 growth just like this one, thoughts on whether its contam or myc, looks more like contam to me.

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Re: Zero growth after 2 days [Re: Mortifiied]
    #29619556 -

Not the growth you want


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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