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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

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OfflineALDER_057
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Registered: 03/14/01
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Re: How many FOAF use Agar culture, SURVEY?
    #295168 - 04/18/01 01:09 AM (16 years, 13 days ago)

AFOAF just started a couple plates of m.e.a with pan cyans. They came from ralphster and fluffy growth was just noticed. AFOAF will keep ya posted. To bad my portabellos that he sent out didn't work, but I had the agar mix wrong. I'll clean the plates(pyrex) and start over on my next day off.



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Offlinekingkc
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Re: How many FOAF use Agar culture, SURVEY?
    #296098 - 04/18/01 02:39 PM (16 years, 12 days ago)

Plates win hands down over any other tek. But ,then, It happens to be ones personal choice. I like research ; thats why I say that.



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Offlinedbs_mother
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Re: How many FOAF use Agar culture, SURVEY?
    #296781 - 04/19/01 07:02 AM (16 years, 12 days ago)

Isolating vigorous homogenous strains is all good. Cloning is all good too, but you have to go back to spores eventually. A strain will degenerate in less than six months from continuous cloning.



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OfflineTrevyn
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Re: How many FOAF use Agar culture, SURVEY? [Re: dbs_mother]
    #296897 - 04/19/01 12:52 PM (16 years, 12 days ago)

Agreed. No question about it. Spores are the beginning to it all. I would point out, however that you can keep a viable strain for much longer than six months with slants, but ultimately, you are absolutely correct.

Peace, Trevyn www.entheogen.st


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OfflineAzure
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Registered: 01/01/99
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Re: How many FOAF use Agar culture, SURVEY? [Re: dbs_mother]
    #297252 - 04/19/01 11:18 PM (16 years, 11 days ago)

Umm, not all strains degenerate after six months...Take the morel for instance-when they form sclerotia in the wild, they sometimes don't fruit for ten years! Also, the matsutake (tricholoma magnivelare) takes a long time before it fruits, like twenty years of growth...



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Anonymous

Re: How many FOAF use Agar culture, SURVEY?
    #297763 - 04/20/01 05:18 PM (16 years, 10 days ago)

Are you germinating spores on the agar? Cloning? What are you using it for? Are you transfering pieces of the mycelium to fresh plates before you inoculate the grain? Once you are assured you have a clean culture, and you practice clean transfers, you shouldn't experience contaminants. It sounds like your mycelium is contaminated, or the scalpel or tools used to transfer are not clean.



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OfflineAnnoA
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Re: How many FOAF use Agar culture, SURVEY?
    #297968 - 04/20/01 10:14 PM (16 years, 10 days ago)

P.bohemica on PDYA.


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OfflineMNmyc
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Re: How many FOAF use Agar culture, SURVEY? [Re: Anno]
    #298080 - 04/21/01 01:24 AM (16 years, 10 days ago)

I'm so jealous... I have no 'nipple dishes'. But I can't deny that agar can save a screwed jar.



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OfflineALDER_057
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Re: How many FOAF use Agar culture, SURVEY? [Re: MNmyc]
    #298096 - 04/21/01 01:53 AM (16 years, 10 days ago)

Anno, That is a nice dish! I surley wish that I could cultivate one of those but unfortunatly that is not a reality unless we see a breakthrough in azure/bohemica indoor cultivation. Have you ever gotten the bohemica to fruit indoors or does that still elude the best?



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OfflineAnnoA
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Re: How many FOAF use Agar culture, SURVEY? [Re: ALDER_057]
    #298255 - 04/21/01 10:27 AM (16 years, 10 days ago)

No, I haven?t cultivated P.bohemica yet, I just discovered and cloned it last October.
I?m not relly in need of cultivating them indoors, I know where to pick them in autumn.
But they can be cultivated outdoors just like P.azurescens or P.cyanescens.

Cultures available versus coverage of p&p costs.

anno.nymer@gmx.at


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OfflineALDER_057
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Re: How many FOAF use Agar culture, SURVEY? [Re: Anno]
    #298334 - 04/21/01 01:25 PM (16 years, 9 days ago)

I appreciate the offer but I am in AZ and it would just not work unfortunatly...lt is the desert.



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Offlinedarkangel932
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Re: How many FOAF use Agar culture, SURVEY? [Re: ]
    #299280 - 04/22/01 11:09 PM (16 years, 8 days ago)

Does using antibiotic agar inhibit the growth of contaminants until it reaches the growth substrate where it can be spread?



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Anonymous

Re: How many FOAF use Agar culture, SURVEY? [Re: darkangel932]
    #299670 - 04/23/01 02:15 PM (16 years, 7 days ago)

You use antibiotics to obtain a culture clean of Bacteria. In theory once that strain is free of the bacteria, you don't need antibiotics any more. It would still be clean without the use of antibiotics. If your jars are contaminating with bacteria, it could be happening at any stage. Mycelium is contaminated, jars are dirty, grain is dirty, air exchange into jars is dirty, any stage. You as the cultivator can spread the contaminants during inoculation. To much moisture content in the substrate, to high of incubation temps. can both lead to mushroom sweating, that yellow exudant, which can feed a bacterial outbreak. Alot of the time this sweating itself can slow down or halt growth without bacteria bieng present. Bacteria replicate extremely fast. If the are present at the grain level, they will definetly express themselves inside of 48 hours. The jar will stink like fermentation, sour, it will be very greasy in appearance.



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Offlinedarkangel932
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Re: How many FOAF use Agar culture, SURVEY? [Re: ]
    #300106 - 04/24/01 12:36 AM (16 years, 7 days ago)

You know something really interesting though is that I had a jar that after shaking, got the greasy appearance. I almost threw it out, but I let it grow for a couple more days and mycelium started growing throughout the jar. This jar is now fully colonized. Does adding too much water make grain "mushy"? Hence giving a greasy appearance?
Back to agar, does dowsing the room with Lysol significantly lower airborne particles? when making transfers... And are you saying it is good to first grow mycelium on a petri plate with anti-biotic agar, then to a non-antibiotic medium to test for possible contaminants?
Thank you





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Offlinesonichell
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Re: How many FOAF use Agar culture, SURVEY? [Re: darkangel932]
    #300200 - 04/24/01 02:12 AM (16 years, 7 days ago)

Note from experience: Bacteria can still grow on ab agar. Mainly fom water accumulation, my friend thinks.



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Anonymous

Re: How many FOAF use Agar culture, SURVEY? [Re: darkangel932]
    #300431 - 04/24/01 11:29 AM (16 years, 7 days ago)

That is what I'm saying. Antibiotics suppress the bacteria just enough to get non infected mycelium at the outer edge of the mycelial mat. Subsequent transfers from this outer edge to new plates should yield bacteria free mycelium. If your mycelium is heavily contaminated your chances of success are greatly reduced.
As far as moisture content, to wet will yield cottony mycelial sectors which grow rapid but produce less pins. It usually will correct itself once cased. The greasy appearance is usually a result of the growth rate and resulting exudants. If it is Bacterial, the mycelium will be slowed or halted on recovery after shaking, and it will have a really sour smell when you open the jar to lay out and case. IF it smells sour, throw it out, don't case. Make sure you have pure spawn to case with, free of contaminants. If it is even remotely contaminated in the jar, it will get way worse when you case.



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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

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