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Offlinethe_guy
Stranger
Registered: 05/12/01
Posts: 22
Last seen: 22 years, 1 month
Starting Agar Culture. Please Help.
    #360972 - 07/25/01 08:54 PM (22 years, 7 months ago)

Ok I am going to start my first agar culture. I bought:
Plain Extra Light Malt- made from 100% Malt Extract. Finely powdered.
Agar- Finely powdered from health store
Yeast- Yeast Lab- Eurpean LAger

20 grams Malt
20 grams Agar
2 Grams yeast

All in petri dishes into a glove box inside a (as sterile as possible walk in closet)

Are these ok ingredients to use?
Please help any suggestions appreciated...


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Offlinezetek
enthusiast

Registered: 04/28/01
Posts: 210
Loc: United States
Last seen: 21 years, 9 months
Re: Starting Agar Culture. Please Help. [Re: the_guy]
    #360976 - 07/25/01 09:08 PM (22 years, 7 months ago)

Yes, those are the right quantities to add to one liter of water. It is probably best to bring the water to a light boil, add your ingredients so they dissolve, and then pc.

One liter of agar pours a lot of plates though. I usually just halve the ingredients and do 500ml.
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Edited by zetek on 07/25/01 10:09 PM.


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