Auto-SummaryThe post details personal cultivation experiences with Psilocybe galindoi, focusing on various aspects such as substrate, temperature, humidity, and handling techniques. Observations include the plant's preference for high humidity, avoiding straw in compost, and its ability to recover from drought. The author also discusses the growth of truffles, pin formation, and the impact of airflow and light on the process. They share their findings on using different substrates, like BRF jars and manure compost, and the challenges of isolating and cultivating the species. The post concludes with a call for feedback and further discussion on the topic.
Private notes on cultivation, observations, guesses/theories, etc.
Here are the first few grows ever working with this variety so this is fun.
None of this is bias, untested, and for the sake of a fun learning experience. If I don't remember users to credit please forgive me.
A lot of help from Stonesun and of course, Workman's notes.
Much love and the credit goes to both of them! Also want to thank Cybesa for the encouragement,
and fun chatting about Ps. Galindoi, much love to them also.
These are experiments after all, totally not an excuse to use if this messed up at all LOL.
Also, beautiful, been wanting to work with this species for a very long time!
The printing is sometimes good, you can usually tell by looking at the gills if they are able to print or not.
Workman worked to get it
its a pretty looking species, etc, etc.
Currently working on whether or not ATL7 will work with an unmodified BOD tub.
Feb.2025-April 2025
temps, during fruiting, spawn run, substrate run.
"68-67 degrees F. lowest" "69-74 degrees F. highest"
No personal experience with any invitro truffles in grain yet. Tolerates popcorn.
Mycelium seemed to "LOVE" corners SOMETIMES of shoeboxes, more fruits/mushrooms on the surface there. Sometimes truffles there too, or
near the edges also.
Snow white, to barely beige, to light tan mycelium.
In grain jars (popcorn, rye) sometimes before reaching 100% in spawn run,
mycelium will display a "lightning bolt" (see photo examp) when this happened usually an indicator truffles are going to form. Not my credit info, saw post here saying the same. Observed it is true. This "bolt" will go white to tanned "maringue" colored. This seems normal.
Pins can take some standing water on surface, and SOME misting, too much or too close and often, WILL cause them to abort.
Gauge, if any osciliating fans on low in the room, or how quickly your terrarium loses RH/humidity. Keep RH and humidity high, with keeping good FAE and vice versa.
Spraying every few hours with oscilating fan on pointed away and NOT directly at terrarium. Remember the longer without misting, fan on or not, and evaportation, micro currents, the humidity will drop significantly, FIX that asap.
Agar with ATL#7 seem way better, less forgiving with thick wedges.
Mycelium on the side, of jar, petri, etc collections from there seemingly less reliable?
ATL#7 isolating seems a bit trickier, due to bacteria and such growing, in the substrate from many mistings and waters. Seems to love to bring bacteria with it, keep in mind when isolating/tissue sampling ATL#7. Try tetracylcin agar(?) dont post that note XD
During colonization, from S2B, a misting/watering was needing during colonization of the substrate. WATER THE UNCOLONIZED Parts/Parts of substrate
on the surface that is NOT colonized, carefully w/spray bottle.
It likes water, do not let it dry out.
IT IS NOT THE END OF THE WORLD, if the casing or substrate dries out, even nearly fully.
Ps.Tamp.Galindoi can recover from droughts (the reason for truffles, duh haha) Dont let it get that bad if fruiting cased substrate.
Doesn't seem to like straw or colonizing it from my observations in a composted manure/straw/coir mixture. HOWEVER it will still tolerate it to some small scale (roughly 25-30% shredded straw in compost)
Also I wanted to test using a pure vermiculite casing layer, this I had no control of but now know it will work. Probably not ideal of course?
What I have heard before is 50/50 coir with washed sand for gardening works well too. That casing layer is said to imitate, the hot and humid, sandy swamps they dwell in, where they fruit from water logged soil, sand, that you imagine goes away with the wind blowing often, but the humidity stays high, its been purported to be tolerant of growing on or near woodchips as well.
Also when spawned to substrate, it will break and crack your casing and substrate apart a bit. That casing layer you worked so hard on to perfect.
Pretty sure that's the truffles growing and "moving around".
BRF jars with ATL#7 (yes you heard me right) spawned to, yup, manure compost, roughly 30% straw, and coir)
Getting colonized BRF jars of ATL#7, spawned to composted manure, straw, maybe some coir, NOTHING ELSE THAT'S IT. Depending on genetics, if spores, LC, agar, stilled produced fruiting bodies, but one or two small truffles. The size of a US 5 cent coin, with the odd appearance of a white chocolate covered raisin.
  Crumbly cake bruh...
Someone else mentioned that here about Galindoi, it doesn't make many truffles when cased with verm (THE BRF cake spawn that is) that is true but I'm still looking into that, kind of in a pinch whether to check on it and break it apart again (Yes it's still growing) or leave it alone.
I was wrong, I guessed ATL#7 wouldn't mind the whole spawning with BRF because it got spawned to manure compost, that THAT would make up for the lack of spawn.
They seem to love/prefer grain, of course right?
if leaving or not able to tend to them, close up the container, keep humid until returned the terrarium that is. Shoeboxes are covered with foil and poked with small holes while colonizing in a dark clean room temp. enviroment.
ATL#7 when grown with grain and S2B with manure compost, when the fruiting/mushroom isolate is used, there are no present pre-truffles that may be present on the surface (when fully colonized or nearly). See Examp.
 
When grown with the isolate "A and B mixture"* (liquid culture) Mushrooms AND truffles can be present, but few to little fruiting bodies.
*A and B isolate mycelium "mixture?" from workman's notes from 2002.
No truffles present on same substrate MINUS the straw.
 This image is a shoebox ATL#7, composted manure and coir. This one is NOT truffle dominant isolate, this one is for mushroom fruiting bodies. I don't know if it will still make sclerotia.
A typical "minitub", "dubtub", or even "shoebox", seemed to last 4-5 weeks before succumbing to trichoderma. For safety reasons the small spot of trich (don't let it get that bad) is removed however you wish carefully. Then if with with a MG or not a fruiting isolate, then when thats when truffles can be harvested. Cleaning is easy, a paper towel to gently clean off bulk from the truffle(s) then another paper towel to get the little crevices and hard to reach spots.
Can be used to inoculate substrate "casing"
Left over grain-spawn from harvesting truffles can be separated and used to inoculate a suitable substrate followed by incubating.
Left over grain-spawn from S2B and in grain jar truffles.
Truffles can "move around" in the casing layer or on surface of substrate.
They can be seen making hills, bumps, and even breaking through the surface to say hi 
Examp. 
I remember getting such an enjoyment outta noticing them moving over a period of days.
Truffles may form (hills, bumps) on surface BEFORE fruits or pins are present.
Pins appeared for me, often 6-14 days after fruiting conditions.
24 hour lighting did NOT seem to affect anything.
Crack the lid, barely open for FAE and keep the humidity still high doing so.
With the lid cracked you will need to mist a little more often, depending on the airflow, room currents, etc.
Truffles seem to form with ATL7 during the substrate run, sometimes continuing with a head start from being in the jars.
Sometimes in my LITTLE experience, other than misting to keep up humidity, the surface of the casing layer can be allowed to dry out a bit WHILE pins are past pinned stage and right before, or during the caps opening up.
IF YOU MUST water in my experience, during pins if the casing is dry, water it like a potted plant, and AWAY from the fruiting bodies (not sure on that one for sure) because thinking about it; the entire substrate is colonized and the mycelium is all connected.
Keep the water away from the pins/fruits if you need to water, the goal is to not let it get too dry before pins.
Dealing with Pan. Bisporus at the time, and reading up as a last ditch resort. Used for ATL too here for an "ATL#7 CAKE" haha I'm having fun experimenting. So spawned more BRF cakes to manure in a little bucket, was not pinning but SEEMED to be focusing on truffles.
Not enough air flow with the container's sides blocking FAE, colonized substrate was "birthed" and treated the same. This was to make room in my terrarium  but I noticed pins finally appeared shortly days after!
Jars with tiny cream colored truffles
Sometimes caps (still convex) may become "blurry" or barely "fuzzy"
suspected culprit is MORE FAE while the caps are getting ready to drop spores, plane out.
Looky!   
You want s'more random shots? Uhhh okay! 
       
Quite amusing cultivating these for research and studying, and I tried a lot of this stuff experimental. I also had quite several jars of spawn to play with. Thoughts? Questions? Inputs?.... concerns? 
    
Edited by Wedgie (09/14/25 09:11 PM)
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they... 
smells like fungi combined toasted grain cereal with marshmallows, or like clean and fresh veggie produce.
if I start a thread on pans, I'll let ya know. 
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