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Lions Mane. Maybe I overeached myself
    #28822298 -

A friend gave me an LC syringe of Lions Mane.  It was old, having been in their fridge for nearly a year.  I'd just sterilised some bags of millet & thought I might as well squirt to LC in and see if anything happened.

It started to colonise, so I sterilised a mixture of wood pellets & soy hulls, still expecting the grain to fail.

The millet's now pretty much fully colonised, but having read through various threads, I think I'm going to have to abandon the idea.  From what I've read:

I need to mix the spawn & the sub together without letting in any contamination - I still use an SAB, so have serious doubts as to whether this would succeed.

I need a Martha to fruit them - I don't have space and the idea of buying a tent, humidifier, fans etc. just in case I get away with the spawning process is a non-starter.

Does anyone have any ideas, even if yield is likely to be abysmal?  Maybe just try to fruit the spawn?  Put the spawn bag in a tote & keep misting in there?  It seems a shame to dump a 6lb bag of fully colonised millet, but if it's time to cut my (minimal) losses, so be it.

Thanks


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Re: Lions Mane. Maybe I overeached myself [Re: Janus62]
    #28822343 -

Don't fret. It's easy enough to inoculate a substrate bag anywhere. They're pretty resistant to contamination. I take blank blocks to the farmers market and them in front of the customer when they want a grow kit and this has a very high rate of success.

The instruction for fruiting I give is to set the block on the counter and mist occasionally, and this works. Put it in a water tub or sgfc if you want some fruiting conditions.

You can inoculate 15 or so 10lb blocks with that spawn, so if you want to keep your tests going inoculate the substrate you made and then reseal your spawn and keep it in the fridge until ready for more.

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Re: Lions Mane. Maybe I overeached myself [Re: deadmandave]
    #28822555 -

Thanks so much.

I used the whole syringe as I didn't think it'd be viable and only wanted to sacrifice one grain bag.

So, just to check I've understood.

I mix some of the colonised millet with the sterilised wood/soy hull mix - in the open air? (That's the bit that's thrown me).  Put the rest in the fridge whilst I sort some more substrate and see how the first grows, then inoculate another with millet etc.?

That would be awesome, which makes me think I've got it wrong :laugh:


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Re: Lions Mane. Maybe I overeached myself [Re: Janus62]
    #28823173 -

Yeah, use the grain to inoculate the bulk sub. Do it as clean as possible but open air in this situation is not so bad. Use alcohol to wipe the bags and your hands and work smoothly, keep the bags open for the shortest amount of time possible.

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Re: Lions Mane. Maybe I overeached myself [Re: deadmandave]
    #28823470 -

Fabulous.  Thank you again.


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Re: Lions Mane. Maybe I overeached myself [Re: Janus62] * 1
    #28825396 -



You’ll be fine fruiting in open air. This is happy in a bathtub, and I have another happily hanging out outside  in a sgfc in the bushes.

I use 1 lb of spawn to 6lb substrate to speed up colonization


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Re: Lions Mane. Maybe I overeached myself [Re: space.madness]
    #28831025 -

Thanks for the confirmation.  I'm hoping to get the chance to give it a go tomorrow.  I'm really hoping it works because they look beautiful, but I've never eaten them.


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Re: Lions Mane. Maybe I overeached myself [Re: Janus62] * 1
    #28831448 -

Janus62 said:
Thanks for the confirmation.  I'm hoping to get the chance to give it a go tomorrow.  I'm really hoping it works because they look beautiful, but I've never eaten them.


they do kinda taste like lobster and are good eating.
I wouldn't over-eat the first time, they have great laxative effect you cut mostly down when you cook them.


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"Sometimes the only way for a man to find true happiness is to take drugs in a group" - Cochise

No makin funna my pomes!

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Re: Lions Mane. Maybe I overeached myself [Re: curenado] * 1
    #28832309 -

Even if you see little olive green patch, do not throw it directly.
I seen multiple time lion mane eating green mold where my oyster struggled. (I made even made a thread about it because it blew my mind).
If the mold is trico, it is dead... But with aspergilus/penicilum, I had great success just letting the myc longer in incubation. They ended fully white and gave a nice yield, not so different from other non contaminated bags.

But if you want nice fruit, you will need humid environment, they tend to dry easily. In my FC, they are the first to become yellowish if the humidity is not well ajusted

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Re: Lions Mane. Maybe I overeached myself [Re: GRETO]
    #28837405 -

Thanks again guys.

I've still not had time to get to it yet, but hopefully at the weekend.

I have this

and this

I'm thinking - pour spawn into sub bag, mix thoroughly, split between the 2 bags, put a few small slits in the sides, put into a large water tub to keep as much humidity as I can without a tent.  It troubles me that the sub has nutrient content that won't be locked away by myc, but I'll do it ascleanly as I can & "nothing ventured, nothing gained".

I was also wondering if it'll be worth getting a piezo humidifier disc to put in the tub?


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Re: Lions Mane. Maybe I overeached myself [Re: Janus62]
    #28846000 -

A lot of guys here report being able to find cheap ultrasonic humidifiers in thrift stores during the summer. Those units have the piezo humidifier disc and a fan inside of it, which will bring in humidity and fresh air.

If you add only a disc to your tub, you will only get the humidity with minimal air movement.


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Re: Lions Mane. Maybe I overeached myself [Re: space.madness]
    #28850106 -

Thanks man.  I've still not taken the plunge, so it's getting pretty solid now.  Rather than let it dry out & die, I'm going to mix it with the substrate this weekend, re-bag it into a couple of bags, put some slits in, and put them in a water tub.  If it contams or fails to fruit, so be it.  I'll consider a martha or similar for my next attempt (if I do it again).


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Re: Lions Mane. Maybe I overeached myself [Re: Janus62]
    #28899431 -

Hi again.

I eventually got round to mixing the grainspawn with the sub (master's mix recipe) and filled 3 unicorn type bags, putting a couple of slits in each.

I put them in a water tub and they finished colonising the sub a few days ago.  I also bought one of those piezo mist makers (well, 4 so far and 2 power supplies) and put it in a bowl of water in there.

I have fruits growing through the slits, but they're sad looking little  things so far, likely because every one of the misters has been unreliable.  They seem to work for a short while when run for 15 minutes every few hours (not been worth sorting a timer yet) before stopping.  Sometimes they work again after drying out completely, as I've found when the next one fails.

I think I'm using them right, immersing them so water covers the sensor that sticks out of the top, so I can't work out why they fail.  It might just be that they're cheap and nasty.

So my question is, what do you guys use?  A particular brand or specification?

Many thanks.


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Re: Lions Mane. Maybe I overeached myself [Re: Janus62]
    #28900297 -

House of hydro units

Or

Amazon humidifiers with non electronic controls. You need the knib type so it will work on an external timer. If it has electronic controls, you will need to press a button manually to turn it on. Will also have a ductable exhaust port, and top filled


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Re: Lions Mane. Maybe I overeached myself [Re: space.madness]
    #28902747 -

Update.

I know this is really ghetto, but I ended up putting a bunch of small holes in the top of my tote with a computer fan sitting on them blowing inwards, and the lid loose and slightly off centre to give lots of FAE.  I started with piezo mist generators, but they were unreliable, so I bought a reptile fogger and connected the hose through a hole in the side of the tub (melted with my trusty soldering iron).

It now looks like the photos below.

I'm astonished I've got this far, but delighted.  I've grown more actives than I know what to do with and having got the growing bug, this looks like a way forward.

I think I'd like to try from 'scratch' though.  Do I wait for the long tendrils and take a spore swab, or should I take a tissue sample from deep inside and clone on agar?

Thanks again all.



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Re: Lions Mane. Maybe I overeached myself [Re: Janus62]
    #28902767 -

Nice job. I rewired my humidifiers so the internal fan is running continuously on high, and the fogger is controlled by the adjustment knob. This allows me to eliminate a separate fresh air fan, with the ability to control pooling.

I would do both cloning, and spore germination because you never would know how aged your cloned culture is.

The spores will yield you a younger and possibly a more vigorous culture. But I would definitely still do the clone, just in case


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Re: Lions Mane. Maybe I overeached myself [Re: space.madness]
    #28902841 -

Thanks so much for the quick reply and positive feedback. 

It's doubled in size since this morning, so I've just prepared a batch of plates.

It's as exciting as my first PF cake! :grin:


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Re: Lions Mane. Maybe I overeached myself [Re: Janus62]
    #28920546 -

Just a quick update in case any of you guys check back on this thread.

I'm really impressed by the fogger https://www.amazon.co.uk/gp/product/B081X5VBVD/ref=ppx_yo_dt_b_search_asin_title?ie=UTF8&psc=1
A full tank running for 15 minutes each hour lasts nearly a week.  When it's running, the extremely fine mist is like a cloud of fog that swirls due to the computer fan before exiting through the offset lid.

All 3 bags have now fruited.  I've just harvested a second flush from one, and the others aren't far behind.

I tried cloning, but found it almost impossible to pick pieces out due to the texture of the insides.  I ended up with 6 plates that were so badly contaminated that it wasn't worth trying to clean them up.

Swabbing a couple of days ago from as far underneath as I could get the cotton buds seems to have worked better.  2 plates are still pristine, suggesting I didn't get anything, but the other 4 are showing growth with no obvious contamination yet.  I'll leave them to grow out a bit and then do a few transfers in the hope of getting something clean enough for LC (I've tried using agar to inoculate bags in my SAB, but failed spectacularly)


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Re: Lions Mane. Maybe I overeached myself [Re: Janus62]
    #28923687 -

Congrats on the grow Janus. Im trying to do something similar with my old lion's mane LC. Appreciate the follow up for us lurkers.:thumbup:

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Re: Lions Mane. Maybe I overeached myself [Re: Janus62]
    #28924080 -

How big are the hole(s) to let air in from the fan?

Is the air exiting through the lid of the tote slightly like cracked open or how do you have it set up?

Another question, how long/often are you letting the fans run?

Edited by sigarettes (08/26/24 12:35 AM)

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Shop: Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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