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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: maximizing yeild [Re: DERRAYLD] * 1
    #28836138 -

lol 200-300 transfers... Aint nobody got time for dat


--------------------
~ I used to get high on life, until I realized life was cut with morons ~
* You need 2 wake up and smell the music! *
- We are all computer data in a materialistic world -
| Sometimes you have to lose yourself, to find anything |

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Re: maximizing yeild [Re: Blazer420]
    #28836148 -

Yes you do have time for that.  Making and testing isolate monocultures , just to get exactly what you want for your set up takes that much.


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The better guide to grow mushrooms.
https://www.shroomery.org/forums/showflat.php/Number/8553541

Beware the power of one.  The power of two. The power of many.

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Re: maximizing yeild [Re: vicepope]
    #28836156 -

:babypalm:

So you suggest we isolate monocultures to find the best yield?

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Re: maximizing yeild [Re: vicepope]
    #28836157 -

there ARE no "max yields", what do you OP need all them max yields for?

1. THis takes several months

2. Isolating requires in many cases either a SAB, LAB or FH

3. Yes people will scorn me but MS PF TEK will give you more mushrooms than you need for the correct purposes. THis isnt a microwave crack baking operation.


--------------------
The PF Tek is my area.:oldman:

Where all anonymity is lost, there cannot exist a person.

Where one cannot avoid to share, there is God

www.zamnesia.nl
RustyWhyte is Commercial!



Edited by Cakewonder (07/03/24 08:13 AM)

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Re: maximizing yeild [Re: DERRAYLD] * 1
    #28836175 -

Isolation sucks if you want to get "high" yields.

Source: https://www.shroomery.org/forums/showflat.php/Number/25464771#25464771

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Re: maximizing yeild [Re: Cosmyc]
    #28836239 -

Holy hell, this is going off the rails quick...

You need excellent sterile technique, you need to know agar- there's just no getting around that one.  Sure, there's a lot of learning behind those two things.

isolate mono-cultures?!? Really?!? Please, say more.

Edited by DrunkPunk (07/03/24 07:17 PM)

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Re: maximizing yeild [Re: DrunkPunk] * 1
    #28836251 -

https://www.shroomery.org/forums/showflat.php/Number/27426583

Read this thread, especially the begining. He says that the amount of water in your substrate dictates the yield possibilities.
The more water the more yield.
And he explains how to get the better yields, by cloning(like, clone one of the mushrooms in that cluster you mentioned, which will make the next batch cluster more readily).

Good luck


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Re: maximizing yeild [Re: DERRAYLD]
    #28836811 -

DERRAYLD said:
I don't even think I've run 300 plates the entire time I've grown mushrooms


That's a relief.  I got the big 500 plate box and was worried I'd need to get a couple more boxes if I'm ever gonna get anywhere :laugh:


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
Hitchhiker's Guide to the Shroomery
Do not be concerned with the fruits of your actions, just give and do your best.
Shunryū Suzuki, Zen Mind Beginner's Mind

I start to laugh at the cosmic humor of this grumpy meat goblin scowling at everything and carrying around petty resentments like treasured turds.

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Re: maximizing yeild [Re: ArkaNaut] * 2
    #28837353 -

Hell no, 300 plates just to find a yielding culture is just plain wrong and a total waste of time.

If you can't find what you're looking for in a couple plates then start again.

There is really no value in running hundreds of plates if you can't stream line and focus your workflow, more is not always better.

Originally I had 10 glass petris for years and made them work.
It's good practice to limit your plates in my opinion,  really forces you to focus.

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Re: maximizing yeild [Re: DERRAYLD] * 3
    #28837371 -

:whathesaid:
Get clean cultures, run them, clone , run them


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: maximizing yeild [Re: cronicr]
    #28837385 -

get another plate, fill it with mushrooms, then clone that son of a bitch.


--------------------
The PF Tek is my area.:oldman:

Where all anonymity is lost, there cannot exist a person.

Where one cannot avoid to share, there is God

www.zamnesia.nl
RustyWhyte is Commercial!



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Re: maximizing yeild [Re: DERRAYLD]
    #28837476 -

DERRAYLD said:
Originally I had 10 glass petris for years and made them work.
It's good practice to limit your plates in my opinion,  really forces you to focus.


:takingnotes: Good idea, it's so easy to buy petris or sauce cups in bulk that you forget what your objective is (getting fruit).

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Re: maximizing yeild [Re: cronicr] * 1
    #28837676 -

cronicr said:
:whathesaid:
Get clean cultures, run them, clone , run them


They think this sounds too simple, if they only knew.

Have you guys considered fannig? Maybe some computer fans powered by a flux capacitor run on horse apples and ultraviolet light? :howyoudoing:

Reddit says that mixing honey with cornstarch is the way to go....



:vibin:

:lol:

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Re: maximizing yeild [Re: DERRAYLD] * 1
    #28837687 -

DERRAYLD said:
Hell no, 300 plates just to find a yielding culture is just plain wrong and a total waste of time.

If you can't find what you're looking for in a couple plates then start again.

There is really no value in running hundreds of plates if you can't stream line and focus your workflow, more is not always better.

Originally I had 10 glass petris for years and made them work.
It's good practice to limit your plates in my opinion,  really forces you to focus.


This.  All day. I've been using 8 plates for the past 2 years, works great, and DERRAYLD is absolutely on point it- focuses my process and I time everything around when I have a clean culture in hand.  Like this guy: 



T1 or 2nd transfer.

If you can't do it in 10, you sure as hell can't do it in 300.


--------------------
Hichhiker's Guide To The Shroomery

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Re: maximizing yeild [Re: DrunkPunk] * 4
    #28837705 -

RR and his "isolate" nonsense. There are no "isolates" achieved by selecting "sectors", this isn't a thing, at least not in the way it's described 99% of the time on these boards. Verum knows better than most that making an asinine number of transfers will get you no closer to an "isolated" single strain.

Single strain isolates are not achieved by macro manipulation of genetic material so blended that actual mycologists in a laboratory require chemical dedikaryotization to separate. Your dirty dick beaters and your dull ass scalpel blade will never separate genetically
distinct parental neohaplonts from a plate culture; if you could, we wouldn't require serial dilution, monosporic isolation, or dedikaryotization by chemical, broth, or protoplast manipulation.

Sayimg that "most isolates suck" should be translated to "most fucking cultures suck", the only "isolation" being achieved is isolation away from contamination (pure culture), or the isolation/selection of leading growth with faster cell division.

Tip: Pure culture is a monoculture. Monoculture does not in any way refer to a single starin other than to describe single species/cultivar propagation, not a synonym for either: monosporic isolate or single strain isolate.

Any culture free of contamination is a monoculture

The central point of inoculation on your plate contains a microscopic world of blended genetic material that grows radially outward from a branched network of tightly woven hyphae, you will never be able to significantly reduce the available genetic material at the macroscopic level, no matter what side of the plate you choose from, no matter how different it may appear at the resolution of your dirty titty peepers.

Furthermore, the genetic diversity (heterozygosity) of your common domesticated lineages are as close to zero as they can get due to the haphazard proliferation of spore prints approaching whats likely closer to f100 than f1 or f2; but, even when some noob makes a new "fill in the blank stupid new x name" their "crosses" are likely unstable f4 max, crossed with other severely reduced cultivars with near zero heterozygosity.

The true reduction of genetic material takes place every time a cultivator takes a print or a swab, selecting for the genetic traits expressed by that particular basidiocarp. This genetic reduction continues along the trajectory of that selection process whether it be intentional or otherwise; once this selection process (successive generational spore collection, not plate transfer) deletes genes, they're gone, for better or worse, so be careful what you select for.

Unless you're quite intentional with your selection parameters you're more likely to produce garbage offspring than something stellar, and that my friends, is why most cultures fucking suck, or conversely why they rock....that and you're probably not using "max yield bins":rofl:

Getting max results means maximum attention to detail including careful appropriation of genetic spore stock or live culture from reputable individuals, then maintaining that genetic material with intentional action. The introduction of wild genetic material should be considered when attempting new and exciting lineages or when attempting to revive old/overworked lines suffering from the deleterious effects of less thoughtful cultivation practices.

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Re: maximizing yeild [Re: Stipe-n Cap] * 6
    #28839076 -

:takingnotes:

  • dirty dick beaters
  • dull ass scalpel blade
  • dirty titty peepers


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
Hitchhiker's Guide to the Shroomery
Do not be concerned with the fruits of your actions, just give and do your best.
Shunryū Suzuki, Zen Mind Beginner's Mind

I start to laugh at the cosmic humor of this grumpy meat goblin scowling at everything and carrying around petty resentments like treasured turds.

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Re: maximizing yeild [Re: Stipe-n Cap]
    #28839099 -

To be fair RR would refer to them as single sector isolates more often than not , he didn't care for the word strain but would use it when trying to explain shit to noobs


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: maximizing yeild [Re: cronicr]
    #28839170 -

Roger that, perhaps a was a tad over zelous with that comment.

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Re: maximizing yeild [Re: Stipe-n Cap]
    #28839242 -

Naw I get it lol, a lot shit back then drove me nuts it was crazy a few years ago arguing about clones having multiple strains ect


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: maximizing yeild [Re: cronicr]
    #28839285 -

Nah guys... Let's just run hundreds of plates, that's how you get max yielders..:lolsy:. :ilold:


--------------------
~ I used to get high on life, until I realized life was cut with morons ~
* You need 2 wake up and smell the music! *
- We are all computer data in a materialistic world -
| Sometimes you have to lose yourself, to find anything |

Extras: Filter Print Post Top
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