Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Hunting and Identification
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   MagicBag.co All-In-One Bags That Don't Suck   North Spore Cultivation Supplies   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy

Jump to first unread post Pages: | 1 | 2 | 3 | 4 |  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Re: More SF pans (cints?) [Re: lowbrow] * 1
    #26021997 -

lowbrow said:
He supplied me with a string of letters he knows I have no way of translating.


It's two DNA sequences. Google for an alignment tool and plug both of those into it. It's very clear that there's several substitutions and insertions. Thus it's very clear that the Pan. sp. one finds growing in grass is not Pan. cinct..

lowbrow said:
You’re really going against the scientific grain on this one.  Can we see this evidence instead of ‘because I said so’.



Your definition of "polite" is seemingly rather different than everyone else's...


--------------------
The Official Kiwi Cultivators Thread - for those of us looking for New Zealand-specific assistance!

Check my journal for my attempts at growing mushrooms!

"Don't accept that what's happening
Is just a case of others' suffering
Or you'll find that you're joining in
The turning away"

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: Dunedinite]
    #26022003 -

Hey Alan, if no one wants the microscope I'll take it. Don't mind paying for it, I'm struggling to find a cheap one I can use to learn the basics of microscopy

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: chipsandwich]
    #26022023 -

chipsandwich said:
Hey Alan, if no one wants the microscope I'll take it. Don't mind paying for it, I'm struggling to find a cheap one I can use to learn the basics of microscopy


I feel like once cost of shipping over here is factored in you'll almost be at the point of being able to buy one hahaha.


--------------------
The Official Kiwi Cultivators Thread - for those of us looking for New Zealand-specific assistance!

Check my journal for my attempts at growing mushrooms!

"Don't accept that what's happening
Is just a case of others' suffering
Or you'll find that you're joining in
The turning away"

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: lowbrow]
    #26022025 -

lowbrow said:
Longwhitecloud9 said:
lowbrow said:
If you read the thread he was the one being a dick to me.


I've been following this all day man and you just came across as arrogant towards someone who has proven him self a pro in this field. He was just politely expressing his view and you were saying it was trash which imo is a dick move.


No.  I asked for evidence in a formal and fairly polite manner(which I have done before).  He supplied me with a string of letters he knows I have no way of translating.  That is a dick move.  But he’s got plenty of white knights that would take his side even if he stomped a puppy to death and used a pic of it as his avatar.

It’s highly unscientific to post ‘cintinculus don’t grow in grass’ without any evidence.  To say ‘I’m in the process of investigating the possibility of the species we refer to as cinctinculus that grows in well manured lawns being a completely different species’ would be appropriate.


Making a blanket statement without any scientific evidence and when confronted on it you produce a random letter sequence that you know cannot be translated, well defend that if you want, but that’s considered an intellectually dishonest tactic in any book.


If you really want to identify a mushroom and be 100% certain take it to a lab. Give them a DNA sequence and ask them to see if the specimen you gave them matches the sequence. I think he was just trying to make a point because you were already just coming out saying he was blatantly wrong. I'm not saying this because I'm some big Alan fan or some other bs like that. I'm saying this because I think you were being a dick and being rude.


--------------------

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: Dunedinite]
    #26022064 -

Dunedinite said:
chipsandwich said:
Hey Alan, if no one wants the microscope I'll take it. Don't mind paying for it, I'm struggling to find a cheap one I can use to learn the basics of microscopy


I feel like once cost of shipping over here is factored in you'll almost be at the point of being able to buy one hahaha.


Yeah, that's the problem all the ones I can find at a reasonable price become stupidly expensive when international shipping is factored in. Do you know anywhere in NZ I can start? Don't want to jump straight in and purchase good equipment if I find out it's not for me

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: chipsandwich]
    #26022084 -

chipsandwich said:
Dunedinite said:
chipsandwich said:
Hey Alan, if no one wants the microscope I'll take it. Don't mind paying for it, I'm struggling to find a cheap one I can use to learn the basics of microscopy


I feel like once cost of shipping over here is factored in you'll almost be at the point of being able to buy one hahaha.


Yeah, that's the problem all the ones I can find at a reasonable price become stupidly expensive when international shipping is factored in. Do you know anywhere in NZ I can start? Don't want to jump straight in and purchase good equipment if I find out it's not for me


I haven't gotten around to it, but you might be able to email the botany, microbiology or biochemistry departments and ask if you'd be able to use their microscopes. Might even be able to hit up the CELS191 coordinator and ask if you can use the dodgy undergrad scopes they use.


--------------------
The Official Kiwi Cultivators Thread - for those of us looking for New Zealand-specific assistance!

Check my journal for my attempts at growing mushrooms!

"Don't accept that what's happening
Is just a case of others' suffering
Or you'll find that you're joining in
The turning away"

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: Dunedinite]
    #26022133 -

Dunedinite said:
chipsandwich said:
Dunedinite said:
chipsandwich said:
Hey Alan, if no one wants the microscope I'll take it. Don't mind paying for it, I'm struggling to find a cheap one I can use to learn the basics of microscopy


I feel like once cost of shipping over here is factored in you'll almost be at the point of being able to buy one hahaha.


Yeah, that's the problem all the ones I can find at a reasonable price become stupidly expensive when international shipping is factored in. Do you know anywhere in NZ I can start? Don't want to jump straight in and purchase good equipment if I find out it's not for me


I haven't gotten around to it, but you might be able to email the botany, microbiology or biochemistry departments and ask if you'd be able to use their microscopes. Might even be able to hit up the CELS191 coordinator and ask if you can use the dodgy undergrad scopes they use.


Thanks, CELS191 was one of my favorite papers. Would rather play round in the safety of my own home though, I'll keep looking

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: Alan Rockefeller]
    #26022142 -

Alan Rockefeller said:
tacodude said:
Alan Rockefeller said:
Check to see if the spores are smooth or ornamented with a microscope at 1000x.


Got one for me to use? Want to buy me one?


I have an old microscope you can have which works and has good optics but comes with some mechanical issues - it needs a light source so you could use a lamp and a mirror or a LED headlamp, and the stage slowly goes down so you need to prop it up with a stack of microscope slides or something. 

There are also working microscopes that you can use at the Oakland lab.

Well I still want to check the honey fungus... They are growing like crazy right now and easy to find, but no cint.

I saw a couple those wierd red ones and a huge couple inky caps. I'm going try to used dried grass left behind from mowing that's mostly sun dried and some soil from the edge of a redwood basin that I also mixed with redwood in another jar where I put a couple alleni caps I broke then dropped sterile water directly on with the caps and stem bits of the pan in the grass and dirt. Hopefully I see some colonization to play with

Edit: I also half jokingly made the statement as Alan and I are local, but definitely would be down to take the scope as I really want to check the honey mushroom I printed a while back.

Edited by tacodude (05/30/19 06:53 AM)

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: lowbrow] * 2
    #26022182 -

lowbrow said:
No.  I asked for evidence in a formal and fairly polite manner(which I have done before).  He supplied me with a string of letters he knows I have no way of translating.  That is a dick move.


I gave you the DNA sequences of both species!  You could have asked how to interpret them rather than get angry.


Quote:
It’s highly unscientific to post ‘cintinculus don’t grow in grass’ without any evidence.



I gave you the raw data - that's a lot better than pointing to some paper or book.  Most researchers don't share their raw data before it's been published for fear that someone will scoop them.

Quote:
you produce a random letter sequence that you know cannot be translated, well defend that if you want, but that’s considered an intellectually dishonest tactic in any book.


It is not difficult to interpret DNA sequences - you can paste them into the query box at https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome to see what they are related to.  To compare two, you click the "Align two or more sequences" box, put one sequence in each query box and click BLAST.

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: Alan Rockefeller]
    #26022631 -

Really,  you obviously are here to troll, he posted DNA sequencing from the ITS region, it is used to identify to the species level, so, don't be a jerk and try remaing teachable. Alan is trying to show you that they have different DNA sequences and means they are not the same species but something that is undescribed at the moment.


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593

Edited by Doc9151 (05/30/19 04:34 PM)

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: Doc9151]
    #26022691 -

Doc9151 said:
Really,  you obviously are here to troll, he posted DNA sequencing from the ITS region, it is used to identify to the species level, so, don't be a jerk and try remaking teachable. Alan is trying to show you that they have different DNA sequences and means they are not the same species but something that is undescribed at the moment.


I challenged the voracity of a claim.  Calling that trolling is in error.


--------------------
Amanita86 said:
Sui is trying to mod right now.  Kinda like a newborn calf tryin ta stand fer the first time ain’t it..

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: lowbrow] * 1
    #26023108 -

lowbrow said:
Doc9151 said:
Really,  you obviously are here to troll, he posted DNA sequencing from the ITS region, it is used to identify to the species level, so, don't be a jerk and try remaking teachable. Alan is trying to show you that they have different DNA sequences and means they are not the same species but something that is undescribed at the moment.


I challenged the voracity of a claim.  Calling that trolling is in error.


You mean veracity, and you didn't challenge it in any way, you dismissed it out of ignorance, that's not the same thing. To challenge it you would have had to point out faults in the methodology, which you didn't.


--------------------
How to add a picture to a post

Sample ID request - READ BEFORE POSTING AN ID REQUEST!

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: Duggstar] * 1
    #26023114 -

Duggstar said:
lowbrow said:
Doc9151 said:
Really,  you obviously are here to troll, he posted DNA sequencing from the ITS region, it is used to identify to the species level, so, don't be a jerk and try remaking teachable. Alan is trying to show you that they have different DNA sequences and means they are not the same species but something that is undescribed at the moment.


I challenged the voracity of a claim.  Calling that trolling is in error.


You mean veracity, and you didn't challenge it in any way, you dismissed it out of ignorance, that's not the same thing. To challenge it you would have had to point out faults in the methodology, which you didn't.


I’m done with this conversation.


--------------------
Amanita86 said:
Sui is trying to mod right now.  Kinda like a newborn calf tryin ta stand fer the first time ain’t it..

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: lowbrow] * 1
    #26023263 -

lowbrow said:
Duggstar said:
lowbrow said:
Doc9151 said:
Really,  you obviously are here to troll, he posted DNA sequencing from the ITS region, it is used to identify to the species level, so, don't be a jerk and try remaking teachable. Alan is trying to show you that they have different DNA sequences and means they are not the same species but something that is undescribed at the moment.


I challenged the voracity of a claim.  Calling that trolling is in error.


You mean veracity, and you didn't challenge it in any way, you dismissed it out of ignorance, that's not the same thing. To challenge it you would have had to point out faults in the methodology, which you didn't.


I’m done with this conversation.


Lmao ok.

Gets schooled

Throws a tantrum

Has a cry

Runs away

Man your never gonna learn anything with a attitude like that. Just sit down and be humble. These guys are all still trying to teach you despite your poor attitude and they are still giving you tips on how you can interpret the information they've given you. That's more patience than most teachers I've met...


--------------------

Edited by Longwhitecloud9 (05/30/19 05:46 PM)

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: Longwhitecloud9]
    #26023499 -

Longwhitecloud9 said:
lowbrow said:
If you read the thread he was the one being a dick to me.


I've been following this all day man and you just came across as arrogant towards someone who has proven him self a pro in this field. He was just politely expressing his view and you were saying it was trash which imo is a dick move.


Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: DdaShroom] * 2
    #26023510 -

I truly promise I dont mean to add fuel to the fire but for anyone new to the site who is reading this all, there are two ways of learning and accepting info and education.

Since I have joined the site and asked for IDs I have always been humble to learn and thus in return every single person that has helped me and that I have come in contact with has been truly amazing to me and incredibly helpful so not only thank you all, but also, again to anyone reading, carry on in a respectful manner and it will benefit you in the long run.

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: DdaShroom]
    #26023874 -

Wow.  I haven't checked in here in awhile, and I see this drama.  Too funny.

Anyway, I am actually glad to see some DNA data on the 'Lawn-Cincts' as I used to call them.  I have corresponded briefly with Alan in the past, and would gladly send samples from here if he wants.  I hope that the weather in CO normalizes, and we get the return of these guys.

So, is this going to be a new active species?  Panaeolus Lawnii?????


--------------------
The more I see mushrooms, the more I see mushrooms. I swear it gets into you.

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: Gilzman]
    #26023890 -

Panaeolus lawnboii


--------------------
The Official Kiwi Cultivators Thread - for those of us looking for New Zealand-specific assistance!

Check my journal for my attempts at growing mushrooms!

"Don't accept that what's happening
Is just a case of others' suffering
Or you'll find that you're joining in
The turning away"

Extras: Filter Print Post Top
Re: More SF pans (cints?) *DELETED* [Re: Gilzman]
    #26023902 -

Post deleted by stevo

Reason for deletion: .

Extras: Filter Print Post Top
Re: More SF pans (cints?) [Re: stevo]
    #26024074 -

stevo said:
Does the lawn "cinctulus" have abnormally oblique germ pores from your observations Alan?


I need to find the one I sequenced and scope it.

Extras: Filter Print Post Top
Jump to top Pages: | 1 | 2 | 3 | 4 |  [ show all ]

Shop: Mushroom-Hut Substrate Bags   MagicBag.co All-In-One Bags That Don't Suck   North Spore Cultivation Supplies   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Hunting and Identification
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* pan cincts and edibles
( 1 2 all )		 trigger 3,030 25 07/16/10 01:06 AM
by SomeGuy
* help identifing mushrooms
( 1 2 all )		 codyrcollier14 7,600 24 09/28/14 11:02 AM
by ambc
* is it Panaeolus cinctulu? choomanfoo 5,734 11 05/26/13 10:20 PM
by choomanfoo
* Foes or Cints ID [Update for prints] MushroomPantheon 431 3 09/22/13 08:34 PM
by MushroomPantheon
* I think i finally found a Pann cint. Pics. underfliptown 1,204 19 07/10/13 10:29 PM
by Gravija
* Pann cints/ foes. Spectacle 610 5 10/23/12 05:07 AM
by Spectacle
* Pan Cint Preservation noluckhunterr 570 1 05/08/09 10:32 PM
by Engelsblut6
* need ID on shrooms found in SF area
( 1 2 all )		 adamlivesinCA 1,846 21 04/04/08 05:03 PM
by landsnorkler

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: ToxicMan, karode13, inski, Alan Rockefeller, Duggstar, TimmiT, Anglerfish, Tmethyl, Lucis, Doc9151, Land Trout
1,599 topic views. 2 members, 46 guests and 27 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.036 seconds spending 0.011 seconds on 20 queries.