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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: Hyphatic]
    #25912301 -

:vibin:


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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: verum subsequentis]
    #25912314 -

verum subsequentis said:


.



Yo you planning to rob a spore bank or something?!

This tools is fucking awesome !


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:freakout:  " Being challenged in life is inevitable being defeated is optional."

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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: iSMOKErocks]
    #25912363 -

socorex automatic refilling syringe. My favorite tool of the trade.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: verum subsequentis]
    #25912660 -

Don't mind me just saving this shit.


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Nothing breads nothing

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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: verum subsequentis]
    #25913169 -

verum subsequentis said:
socorex automatic refilling syringe. My favorite tool of the trade.


So you use this for administering liquid culture ?


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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: Luminous7]
    #25913305 -

yes sir


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: verum subsequentis]
    #25915455 -

Thanks for the info!:takingnotes:



I know my notebook is magic!
Because it keeps disappearing... :facepalm3:

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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: Dayglo Joe]
    #25915458 -

Dayglo Joe said:
Thanks for the info!:takingnotes:



I know my notebook is magic!
Because it keeps disappearing... :facepalm3:


LMAO


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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: Luminous7]
    #25976626 -

Kickass thread verum! When using a master slant, and transferring a sample, should you let the mycelium grow back out over the spot you removed before putting back in storage? I do understand it doesn't take a large piece to propagate a fresh plate, but i'm trying to wrap my head around handling masters before i make them.

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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: MLPismyOPSEC]
    #25976652 -

There is no hard rule on that. I can tell you to let the slant warm back up before transferring or you'll break a huge chunk out when trying for a tiny piece. Also, i usually don't pc my scalpel before agar work. I just flame the whole thing really well at the beginning of the session. But, when planning on busting out the masters i take every precaution, including putting the scalpel through the pc and attaching a new blade after the pc.

I don't always let the myc grow back over but will if i've taken from it a few times and I'm having to reach deeper in to get the transfer. If you play it right though, you'll only be busting out the master slant once a year or so. This is done by always having a master plate to make new slave plates from. You can make slaves from the master plate until it's almost empty. That's a lot of slaves. Then you can make a new master from the old master. you can carry on doing this until you notice a lack in performance (don't make the noob mistake of fucking something up and blaming the culture) due to age (cell division).


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: Boogieman47]
    #25976685 -

Great write up! I always enjoy reading this. Cheers! :thumbup:

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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: verum subsequentis] * 1
    #25977499 -

verum subsequentis said:
This is done by always having a master plate to make new slave plates from. You can make slaves from the master plate until it's almost empty.


Bingo, that really completes the puzzle. So keep a master slant and a master plate in the fridge, make slaves from the master plate. Change the master slant every 2 years, change the master plate 6 months/1 year, slaves to colonize jars. Thanks so much for the response.

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Re: an answer that turned into a post. slants, masters and slaves, cold storage, clone hunts.... [Re: MLPismyOPSEC]
    #25977551 -

My pleasure


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: an answer that turned into a thread... slants, masters and slaves, cold storage, clone hunts.... [Re: verum subsequentis]
    #26204549 -

Great write up!!!


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JJ

Draw unto others as they have been drawn to you.... WSP

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Re: an answer that turned into a thread... slants, masters and slaves, cold storage, clone hunts.... [Re: newtomyc] * 1
    #26204559 -

Thanks. I pulled a couple cultures out of cold storage and transferred from the master slants to plates. I plan on uploading a bunch of pics of the process soon.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: an answer that turned into a thread... slants, masters and slaves, cold storage, clone hunts.... [Re: verum subsequentis]
    #26213661 -

What a great visual!

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Re: an answer that turned into a thread... slants, masters and slaves, cold storage, clone hunts.... [Re: verum subsequentis]
    #26250646 -

Answered so many questions! Thanks a ton for this!!

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Re: an answer that turned into a thread... slants, masters and slaves, cold storage, clone hunts.... [Re: Stampy]
    #26250655 -

:goodday:


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: an answer that turned into a thread... slants, masters and slaves, cold storage, clone hunts.... [Re: verum subsequentis]
    #26501723 -

Another great thread. Saving :smile:

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Re: an answer that turned into a thread... slants, masters and slaves, cold storage, clone hunts.... [Re: verum subsequentis]
    #26553843 -

bump


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It's not so much that I am the center of the universe, it's that the universe revolves around me...




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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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