badmojo420 said:

wouldnt it smell bad? it smells pretty much the same as the brf cakes. the surface is also dry?

im not disputing your claim just trynig to better understand.

even so, this was meant to be a testing tray for the liquid culture (i didnt label it or anything but im pretty sure its 1:1 spawn to sub) so im not upset about this tray.

maybe i wont consume the fruits but would they be ok to collect spore from for the next round of syringes?

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badmojo420 said:

Tub and tray have been harvested. Unfortunately I didnt get any cool pics. What I did get was about 540 grams wet between the two with a handful of slow pokes on either one.

Big meaty Koh Samui fruits and from my homemade multispore syringe I can clearly see at least 3 VERY distinct genetic profiles that are very clearly different! Two cluster phenos (both of which i printed and took a tissue sample from one), one has a nippled cap and stained so dark it was practically black!

I have 3 test agar jars going right now but Im unsure of my tek and cleanliness so just in case I purchased some sterile prepours from amazon.

3 qt jars of rye already colonizing so i have a head start on my next tub.

I gave both the tub and the tray a VERY good misting as they were both feeling dry.


Probably wont update this thread any longer but I might take a photo of all my dried fruits tomorrow for one last update.

I have to thank everyone here for any help I have received as I know this wouldnt be possible without this forum so thank you all.

Out of curiousity though should I be using a knife to harvest my fruits? They seem pretty deeply rooted and the first cluster tore up a sizable section of substrate probably ruining any chances i had at a second flush.

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Yeah harvesting the pins destroys the substrate. Ive gotten multiple flushes but thats when I twisted the mushrooms off one by one VERY slowly and gently. Minimizing the amount of coir pulled away. I haven't tried using a knife or scissors. Perhaps you can try that and let us know how it goes!

badmojo420 said:

ah well i do have some PDA agar powder so i can actually do that next time.

i start by printing my spores on foil under a glass jar inside my SAB then i will fold it up until ready to use, sometimes kept in a ziploc bag in the fridge (not sure if thats bad or not actually)

from there back into the SAB with a sterilized syringe (boiled water sucked into the syringe through the needle 5-6 times keeping the last one). i flame the needle to sterilize before it goes into the SAB to fill my little cup (alcohol washed) with water which i scrape the spores into with a little flame sterilized knife and i suck the spores into the syringe.

my liquid culture recipe is just 4% light corn syrup (some crystal clear brand, not karo) and it also has a tiny bit of vanilla in the ingredients it says.

i have a half pint jar with two injection ports (self healing from a store not homemade) with syringe airport with poly fill, i pressure cook the LC obviously, 60 minutes at 10 psi or something like that i cant remember (i admit i didnt actually follow a tek but kinda read multiple teks and made it work with what i had). came out clear no cloudyness or anything. inject my spores like i do a pf jar, about half cc maybe 1 cc per half pint jar. 3 days later they have germinated and within a week or two there is a nice cloud of mycelium that i suck up into a syringe that i use to inoculate grain jars with.

i have no more spore prints and 10CC of my homemade LC left so i think ill get right on an agar tek for that last syringe.

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Mr Piggy said:

badmojo420 said:

ah well i do have some PDA agar powder so i can actually do that next time.

i start by printing my spores on foil under a glass jar inside my SAB then i will fold it up until ready to use, sometimes kept in a ziploc bag in the fridge (not sure if thats bad or not actually)

from there back into the SAB with a sterilized syringe (boiled water sucked into the syringe through the needle 5-6 times keeping the last one). i flame the needle to sterilize before it goes into the SAB to fill my little cup (alcohol washed) with water which i scrape the spores into with a little flame sterilized knife and i suck the spores into the syringe.

my liquid culture recipe is just 4% light corn syrup (some crystal clear brand, not karo) and it also has a tiny bit of vanilla in the ingredients it says.

i have a half pint jar with two injection ports (self healing from a store not homemade) with syringe airport with poly fill, i pressure cook the LC obviously, 60 minutes at 10 psi or something like that i cant remember (i admit i didnt actually follow a tek but kinda read multiple teks and made it work with what i had). came out clear no cloudyness or anything. inject my spores like i do a pf jar, about half cc maybe 1 cc per half pint jar. 3 days later they have germinated and within a week or two there is a nice cloud of mycelium that i suck up into a syringe that i use to inoculate grain jars with.

i have no more spore prints and 10CC of my homemade LC left so i think ill get right on an agar tek for that last syringe.

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If you are making LC from spores, that's where you are getting your contaminates.  You have to clean up spores by germing them on agar and making transfers until you have a clean culture.  At that point you can drop a wedge in your LC, or do a tiger drop if you're using pasty plates.

Spores are inherently dirty, no matter what procedure you take.

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