Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
How many transfers from a slant
    #25575620 -

Do you guys generally do a couple of transfers from a culture slant before inoculating a grain jar?

The reason I'm asking is because I just got fucked over with contams. I did a transfer from a culture slant to agar which I inoculated my master grain jar, then did g2g to 9 jars.

I used 8 of the jars to make 2 monotubs which both contaminated, I managed to get some shrooms from one, the other one contamed before any came up.

Using the slowest growing jar from the original 9 I did G2g which all contamed

Because everything ended up getting contaminated I'm guessing it happened early on which is why I'm wondering if I should have done multiple transfers from the slant to ensure its clean.

Thank you!

Extras: Filter Print Post Top
Re: How many transfers from a slant [Re: spiz]
    #25575626 -

I would do at least one or two transfers just to ensure that the wedges that you put in your master jar are clean. Also if you do transfer from a slant to a petri and it is contamed, it makes it a heck of a lot easier to catch and gives you a chance to clean up your culture before you use it to inoculate jars.


--------------------
"..once you get locked into a serious drug collection, the tendency is to push it as far as you can."

Trade Stuff

Extras: Filter Print Post Top
Re: How many transfers from a slant [Re: Nekobasu]
    #25575812 -

Nekobasu said:
I would do at least one or two transfers just to ensure that the wedges that you put in your master jar are clean. Also if you do transfer from a slant to a petri and it is contamed, it makes it a heck of a lot easier to catch and gives you a chance to clean up your culture before you use it to inoculate jars.


So even if you grow out the wedge from the slant and it looks clean you do a couple more transfers to ensure there's nothing hiding?

Extras: Filter Print Post Top
Re: How many transfers from a slant [Re: spiz]
    #25575817 -

I intend to use my slants the same as any other external source: via agar cleanup. No telling what I did wrong years when I made the slant.

Extras: Filter Print Post Top
Re: How many transfers from a slant [Re: spiz]
    #25575823 -

spiz said:
So even if you grow out the wedge from the slant and it looks clean you do a couple more transfers to ensure there's nothing hiding?


Correct! :thumbup:


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

Extras: Filter Print Post Top
Re: How many transfers from a slant [Re: Tormato]
    #25575989 -

I just go from the slant to a plate, then from that plate make another slant and use the rest of the plate as inoculant. Anything more is wasting petris IMO.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Long-term storage: culture slant, LC (aqueous) or grains. M11 5,891 12 07/27/11 10:41 PM
by M11
* culture slants-tighten lids? laughingdog 4,056 15 05/01/11 02:14 AM
by OmSource
* why do we let master culture slants solidify at an angle?
( 1 2 all )		 Wolfgang 3,155 23 12/21/15 12:15 PM
by Josex
* Batch of Petri's and Slants EvilMushroom666 2,237 18 09/23/10 11:14 PM
by Niwita
* Mephisto's Master Slant Tek
( 1 2 3 4 5 6 7 all )		 Mephistophelian 41,460 124 06/10/19 08:54 PM
by Mephistophelian
* Avoiding condensation in slants Hypercube 3,166 6 02/29/08 08:36 PM
by smokedout420
* how do you make slants? xhooliganx 4,442 12 02/23/07 11:35 AM
by Blutjager
* differences betwen strain isolation by transfers and by cloning?
( 1 2 3 4 all )		 FGL 15,795 62 10/06/09 08:13 PM
by feelfunny

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
558 topic views. 13 members, 870 guests and 129 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.011 seconds on 16 queries.