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cc2
Mush

Registered: 05/15/10
Posts: 2,611
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Re: why do we let master culture slants solidify at an angle? [Re: Josex]
#22682895 - 12/21/15 11:52 AM (8 years, 1 month ago) |
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Josex said:
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bodhisatta said: When you make a transfer from slant to slant its still generation 1 for practical purposes. There's so little cell division that its not like a full generation that would eventually lead to performance drop off
And I guess that even if a performance drop-off ocurred it'd be negligible, wouldn't it? and it'd take many years...
right
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: why do we let master culture slants solidify at an angle? [Re: cc2]
#22682906 - 12/21/15 11:58 AM (8 years, 1 month ago) |
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cc2 said: don't want to sound harsh but really this 6+ years thread necromancy wasn't necessary, the answers you're looking for have been provided plenty of times too.
slants are sealed, more agar and less air per volume and are more space efficient than petri dishes. the process about mycelium degenerating after a lot of transfers (or more precisely cell divisions) is called senescence.
Very informative! But you made it sound like I just disenterred a dead body and pissed on it. I'm lousy at finding the information I'm looking for on this website. I promise you that once I come to grips with it I'll stop sinning.
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cc2
Mush

Registered: 05/15/10
Posts: 2,611
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Re: why do we let master culture slants solidify at an angle? [Re: Josex]
#22682919 - 12/21/15 12:05 PM (8 years, 1 month ago) |
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Quote:
Josex said:
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cc2 said: don't want to sound harsh but really this 6+ years thread necromancy wasn't necessary, the answers you're looking for have been provided plenty of times too.
slants are sealed, more agar and less air per volume and are more space efficient than petri dishes. the process about mycelium degenerating after a lot of transfers (or more precisely cell divisions) is called senescence.
Very informative! But you made it sound like I just disenterred a dead body and pissed on it. I'm lousy at finding the information I'm looking for on this website. I promise you that once I come to grips with it I'll stop sinning.
just pushing you to sharpening your searching skills, I've forgiven your sins muchacho
http://www.shroomery.org/forums/dosearch.php slant vs petri - 33 results www.shroomery.org/forums/showflat.php/Number/15936993
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: why do we let master culture slants solidify at an angle? [Re: cc2]
#22682947 - 12/21/15 12:15 PM (8 years, 1 month ago) |
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Quote:
cc2 said: just pushing you to sharpening your searching skills, I've forgiven your sins muchacho
http://www.shroomery.org/forums/dosearch.php slant vs petri - 33 results www.shroomery.org/forums/showflat.php/Number/15936993
That proves that you are already famirialized with the website. I tell you, I've been trying to type different combinations of words for half an hour just to see if I could hit upon an answer to my question... nothing. Congrats on your Spanish
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