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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Four "Time of Death" Questions
    #25552348 -

1. On 10/11, I inoculated 10 WBS jars with lion's mane using a Basswood Farms syringe. Not much action, but here's what's visible:



When should I call “time of death”? Forensic insights? Rays of hope?

2. On 10/12, I spawned (at about a 15% rate) fairly vigorous oyster mycelium growing on straw to some gallon buckets (pasteurized coir + HW sawdust + gypsum), and set them in a tote in a giant, loosely closed but misted black contractor bag (opened 1x+ a day) @ ~68ºF. I don’t see growth through the 1/2" holes (4 per bucket); yesterday I noted little flies buzzing around; today I opened a top and could detect no growth.

I don’t want to call time of death on these yet. Should I tape up the holes? Or sit on my hands and wait some more?

3. On 9/17, I spawned oyster growing on straw to more pasteurized and “gypsumized,” drained straw and tied it off in 14" poly tubing, poked slits for air exchange. I left it in the garage. No visible growth, and now it's getting pretty cold (Zone 5a, VT) . . . I presume it’s time to call it?

4. Almost a month ago, Trich hit the two monos (before my first flush). They continued to produce, but are slowing in the cold. So here, too, I presume it’s time to call it?

I probably hold on for too long, and should rather learn quickly from and ditch failures - and move on to try again. Thoughts?

Thanks for the help!

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Re: Four "Time of Death" Questions [Re: Groovy Hobbit]
    #25552683 -

Hard to tell anything from those pics. Wait until you have more growth and post more pics. Multispore can take a long time. Next time go to agar then to grain. If this spawn works out just save enough grain to do a G2G transferer next time. It will save you a lot of time on your next grow.

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Re: Four "Time of Death" Questions [Re: SHROOMSISAY01]
    #25552727 -

Thanks, SHROOMSISAY01. Yes, before long, I'll have a flow hood - and g2g + agar will be much better . . .

In the meantime, I'll wait for some more growth on the LM jars.

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Re: Four "Time of Death" Questions [Re: Groovy Hobbit]
    #25554026 -

This is a good option for a flow hood hospitals use them in the ceilings and they are pre-built with a fan and ready to use except for a cleaning if you buy a used one. I got lucky and got two brand new ones for $70 each from a place in Cleveland Ohio if you are close to there I will give you the name of the place I got mine from.

hepa ceiling filter...

https://www.ebay.com/sch/i.html?_from=R40&_trksid=m570.l1313&_nkw=hepa+ceailing+filter&_sacat=0

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Re: Four "Time of Death" Questions [Re: SHROOMSISAY01]
    #25557229 -

Awesome!

I'm not near Cleveland; but I'll definitely check local hospitals, and those ceiling filters. Thanks!

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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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