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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Best way to revive an old strain?
    #25397108 -

I have 8 agar dishes set aside specifically for reviving a print or two I have of syzygy. Was told to hydrate the spores then inject them on the agar. Im assuming there will be some contamination issues. Was going to cut out a section that was good if possible and transfer to another agar dish and hopefully get more good than bad after that xfer. Does this sound doable?

Extras: Filter Print Post Top
Re: Best way to revive an old strain? [Re: WoodyCider]
    #25397132 -

Okay... just went digging into my stash of mj seeds where I kept my old prints. I have 2 syzygy prints to work with. 1 unmarked either syzygy or PE and one that is labeled "OR" that clearly was a gift like syzygy but no clue what OR is. Sounds like I have TWO strains to try and revive.... might need more agar.

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Re: Best way to revive an old strain? [Re: WoodyCider]
    #25397157 -

Since you have 8 agar dishes, why not just try using the print normally on a couple dishes and seeing if they'll germinate normally? If they're viable and the agar isn't too dry/old it should work. No need for syringes. I tried this out a couple days ago with a roughly 6 month old cubensis print stored at room temperature and it germinated.

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Re: Best way to revive an old strain? [Re: Its All Energy]
    #25397171 -

Agar dishes are fresh. Just bought 8 specifically to revive syzygy before I knew I had whatever OR is. The syzygy prints are 6-9 years old lol

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Re: Best way to revive an old strain? [Re: WoodyCider]
    #25397240 -

I got some syzygy too man.
Can’t wait to resurrect.
But 6-9 years. Dang that’s old.
.
Get you some sterile water
This is how I’d do it.
PC a jar of water, let it cool, get a syringe, drop one or two drops of the water onto the print, use the syringe to dislodge spores from the print into the drop of water, suck up the solution, drop one drop each into the plates, wrap the plates, tilt the plates back and forth to roll the drop on the agar... thus depositing spores.
Just what I would do.
.
Welcome back to mycology, brother.

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Re: Best way to revive an old strain? [Re: Jabensis]
    #25397288 -

Just make up a syringe and let it sit at room temp for a few days. Then put a drop of solution on an inoculation loop and swipe your plates. Make sure you have some to do transfers.

I doubt you have an actual PE print, but OR is likely Oak Ridge.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Re: Best way to revive an old strain? [Re: 36fuckin5]
    #25397885 -

Yeah, i remember pe was like impossible to print but thats funny you say oak ridge because that was the only thing that was popping up in my head. Guess my memory isnt all that bad

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Re: Best way to revive an old strain? [Re: WoodyCider]
    #25398014 -

Ive seen PE prints.


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Best way to revive an old strain? [Re: bodhisatta]
    #25398340 -

bodhisatta said:
Ive seen PE prints.


I have too, I've even made a couple. But they're rare.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

Extras: Filter Print Post Top
Re: Best way to revive an old strain? [Re: 36fuckin5]
    #25398896 -

Right on!

I just bought a new PC that can run anything Ill ever throw at it. I also managed to find two sites, one sells syzygy, and one sells oak ridge so I figured id buy a syringe of each hoping for an "insurance" policy.

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