Penis envy isolation part 3

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verum subsequentis
seeker of truth

Registered: 03/22/16
Posts: 8,732
Last seen: 4 years, 5 months

Penis envy isolation part 3

9
#25178416 - 05/01/18 07:08 PM (8 years, 6 months ago)

Alright, this is the continuation of THIS which was the continuation of THIS.
long story short, isolation is taking me a lot longer than i thought.

i had very strange reaction this time. i made up a bunch of 2% mea and a few 1% mea. i just wanted to be able to see how the same (semi isolated) cultures would preform on the two. so i took transfers from what appeared to be the same sectors and put them to both. i normally do 3% mea so the results were a little surprising. the red is 2% and the blue is 1%.

i feel like i got carried away with the food coloring on the red and they didn't kike it at all. some cultures did better than others but they all struggled compared to the 1% and compared to my normal 3%.

plate fuckin 40
1% MEA

edit 5/16/18
PLATE 41
2% MEA
sideways for some fucking reason

Edit 6/4/18

I got busy and forgot to upload last week's transfers so here they are.
P42

Some of them are isolated and some others may be but I'll watch them for another transfer to be sure.

And here's this last round
I had to leave town for a few days so these went in the fridge and ended up with some condensation problems on a few plates. Quite a few of them have appeared to be isolated for a couple transfers and will be going to grain soon
P43

Alright, sorry folks I got a little busy and overwhelmed for a minute there and haven't been faithful to this thread. BUT, here we go

Plate 44

Plate 45

Plate 46

so, it's now june 23rd 2018 and all isolates are either in grain (milo)

or already cased and in "fruiting conditions" in bags that are placed in tubs.

some cultures have been isolated for four or five transfers but i just wanted to be sure that i knew i was done so i kept transferring to see if they'd start sectoring again. if you've been around since the beginning you may remember me saying that i thought i was almost done way back at transfer 19. that's because sometimes a culture will look very uniform for a couple of transfers and then blam, sector city all over again.

once i knew that a handful of them were isolated i put that handful to grain and slanted each isolate. each isolate went to it's own quart. once fully colonized those were g2ged into bags. bags were 2 quarts grain and 2 quarts cvg, ham crammed 12 at a time into my aa941 and pressure cooked at around 18 psi for four hours. each of the bags recieved a quart of colonized spawn. making each bag 2 quarts cvg and 3 quarts spawn. once colonized the tops were cut off, the bags were cased with properly pasteurized peat/verm with hydrated lime added at 1/2 tbsp per quart of dry peat and placed into montubs with polyfil stuffed tight on bottom and super loose on top. 6 bags per 110 quart tub.

the slants are in the fridge

i lost a jar or two and a couple bags didn't make it but i have them slanted and if need be I'll pull them back out and give them a transfer or two and another go.

i kept transferring the cultures that were still showing sectors and then quit at plate 46 because most of them where isolated and i was fine with a few of them only being near isolated. these plates were all cleaned, extra cling wrapped, wrapped in foil, stuck in ziplock gallon bags and placed in the fridge. i needed to wait a while because i had too much going and didn't have room to fruit them.

a day or two ago I pulled them all out, made up 45 quarts of milo, washed a shitload of jars and did the whole thang again.

round two

BEHOLD

it is time to shake that ass

edit 8/4/18
Alright ya'll
i made up 80 pounds of WBS

and 9 servings of CVG

and ended up with a lot of shit to process

twelve bags at a time I've been loading up the ol AA and giving them four hours at pressure.
I'm in the middle of my third pc cycle and I'll do my fourth in the morning.

And then these little fellas all get there own bag

Edited by verum subsequentis (08/05/18 01:00 AM)

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