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Invisiblemicro
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Registered: 05/09/03
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Claviceps purpurea sclerotium in vitro?
    #2045133 - 10/26/03 05:47 PM (14 years, 6 months ago)

There's a discussion about this on the hive, and I was wondering if anyone has tried to form sclerotia by starving or cold shocking the mycelium on a solid rye substrate. It seems like the mycelium forms sclerotia in a parasitic manner in the ovary of rye, but is it impossible to just expose it to adverse conditions to try and get sclerotia?

Thanks,

--
Micro


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Anonymous

Re: Claviceps purpurea sclerotium in vitro? [Re: micro]
    #2045138 - 10/26/03 05:49 PM (14 years, 6 months ago)

It has yet to be done succesfully by pharmaceutical companies with oodles of cash and equipment.


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Offlineetard
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Re: Claviceps purpurea sclerotium in vitro? [Re: ]
    #2045197 - 10/26/03 06:07 PM (14 years, 6 months ago)

Just think about how nice that would be? I would not have to wait another five years to get a fake hit again.....God dammit.


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Invisiblemicro
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Re: Claviceps purpurea sclerotium in vitro? [Re: etard]
    #2045206 - 10/26/03 06:11 PM (14 years, 6 months ago)

Lol -- I wouldn't actually eat the ergot, unless you want you arms and legs to fall off (and, oh yeah -- die.)

Funny story -- this guy in the 1600's was so convinced that ergot was not the cause of ergotism he ate a cup full of ergot. Needless to say he died the next day (and probably had one hell of a trip....) (out of a mycotoxins textbook)

--
Micro


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(Avatar is Maxxy, a character by Mizzyam, RIP)


Edited by micro (10/26/03 06:13 PM)


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Offlinestara
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Re: Claviceps purpurea sclerotium in vitro? [Re: micro]
    #2049740 - 10/28/03 06:01 AM (14 years, 6 months ago)

Is there any dosage for ergot ;too less to die and too much to trip?


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OfflineRaadt
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Re: Claviceps purpurea sclerotium in vitro? [Re: stara]
    #2050456 - 10/28/03 02:05 PM (14 years, 6 months ago)

^ ^ ^ "too little to die, and enough to trip"


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-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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OfflineEkstaza
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Re: Claviceps purpurea sclerotium in vitro? [Re: Raadt]
    #2050519 - 10/28/03 02:23 PM (14 years, 6 months ago)

Well one might live after proper medical treatment and they might also learn to get along quite nicely without fingers and toes.


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OfflinePsily_Rabbit
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Re: Claviceps purpurea sclerotium in vitro? [Re: Ekstaza]
    #2053777 - 10/29/03 03:48 PM (14 years, 6 months ago)

Don't try this at home, thats all i can say


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Weakness or power?
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All go hand in hand, you choose your path.


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Invisiblealtarego
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Re: Claviceps purpurea sclerotium in vitro? [Re: Psily_Rabbit]
    #2056399 - 10/30/03 07:51 AM (14 years, 6 months ago)

Check out this link:

Ergot

I consistently find ergot sclerotia in my bulk organic rye bags. Once I tried cold shocking, surface sterilizing and fruiting them on water agar, but to no avail. It was a single experiment that did not work, and is well worth looking into further.

- AE


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InvisibleDazedSol
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Re: Claviceps purpurea sclerotium in vitro? [Re: ]
    #2063303 - 11/01/03 07:44 PM (14 years, 6 months ago)

I honestly dont know....so i'll ask :smile:

Isnt pharaceutical grade ergotamine tartate synthetically produced? Pharm companies dont actually grow ergot contamed grains do they? 


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Invisiblemycofile
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Re: Claviceps purpurea sclerotium in vitro? [Re: DazedSol]
    #2068070 - 11/03/03 03:27 PM (14 years, 6 months ago)

I don't know if Pharm companies do, but birdseed companies sure seem to :wink:  (although in all honestly I never truly id'd the sclerotia, the cheap birdseed was full of sclerotia of some type).


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PM me with any cultivation questions.

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