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Wu-tang
C20H25N3O



Registered: 10/02/16
Posts: 269
Last seen: 6 years, 7 months
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Liquid Culture For LSA Production of Claviceps Purpurea?
#23761994 - 10/22/16 07:28 PM (7 years, 3 months ago) |
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So I read if you cut Claviceps purpurea into pieces you can spawn it on agar and from that develop mycelium. If you took a wedge of Claviceps purpurea agar mycelium and dropped it in some sterile liquid culture could you effectively produce the ergot alkaloids? I read ammonia is moderately basic a 1.0 M aqueous solution has a pH of 11.6 so could you add ammonia to the fully developed liquid culture to free base the alkaloids and extract with say chloroform? Then could combine all the chloroform pulls in a tartaric acid solution to yield ergot tartrate. Although im not sure if Claviceps purpurea mycelium has a large content of LSA but it would be a non traceable way to obtain enough highly pure LSA to convert to LSD. But would you use the same tek as this? https://www.shroomery.org/10477/Cybers-Liquid-Culture-Tek I figured the mycelium would contain some LSA and you can keep making jars for basically an infinite amount of untraceable LSA.
-------------------- 400 years ago-The earth is the center of the universe and anyone who says outherwise is a heretic. How dare you claim that the earth is not the center of the universe? Your looking glass your moons around Jupiter and your navigation tables prove nothing. Your heresy is an affront to the church and to God who made the earth the center of the universe. Now-All drugs that can expand consciousness are without medical or social justification and anyone who uses them is a criminal. How dare you claim that an understanding of God is to be found in a white powder? This talk of communication with the inner self, the finding of one's way into the hidden reaches of the unconscious, is New Age nonsense and simply an excuse to use dangerous drugs.
Edited by Wu-tang (10/22/16 07:28 PM)
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hjalmar
chemist, autist


Registered: 10/27/04
Posts: 295
Loc: Belgica Secunda
Last seen: 9 months, 25 days
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Re: Liquid Culture For LSA Production of Claviceps Purpurea? [Re: Wu-tang]
#23773347 - 10/26/16 01:34 PM (7 years, 3 months ago) |
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Yes, I don't really understand your question, but yes if you follow the conditions published you can replicate their experiments yes. That is the meaning of modern science and their reason so you can retry and even publish their confirmed experimental conditions.
You are not the first man growing an organism into defined, confined conditions and being able to perform a standard acid/base alkaloidal extraction from it and isolate a pure compound by using a standardised procedure, yes.
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hjalmar
chemist, autist


Registered: 10/27/04
Posts: 295
Loc: Belgica Secunda
Last seen: 9 months, 25 days
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Re: Liquid Culture For LSA Production of Claviceps Purpurea? [Re: hjalmar]
#23773361 - 10/26/16 01:39 PM (7 years, 3 months ago) |
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just use all precautions as you would for a vulnerable compound:
shield from UV light, use an Argon blanket (welding supply store), and you'll be fine
- argon is heavier than air, so it will push out any remaining oxygen
Edited by hjalmar (10/26/16 01:40 PM)
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
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Re: Liquid Culture For LSA Production of Claviceps Purpurea? [Re: hjalmar]
#23773862 - 10/26/16 04:31 PM (7 years, 3 months ago) |
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Read published literature on cultivation, selection, and mutation. It's not an easy task. Most wild strains produce little to, appreciably, no ergolines in LC/agar. If you were to just take a wild specimen without screening and grow it in a LC like mycilium you would probably be lucky to get 10s of mg of lsa per liter.
Ideally you will have ~100 separate strains and take the top 5-10 performing ones. From there you will mutate them to increase the yield yet further. Then you will adapt your medium to something that works well with the selected strain. Each process takes a lot of time, trial, and error. I wouldn't even attempt it without a working lab. By lab I don't mean some breaking bad shit but some basic reagents, glasswear, and understanding of how it all works. If you just want 1g or so of precursor you are far better of going with some of the ergoline containing seeds I would bet.
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