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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Lowest power on microscope?
    #2015437 -

What would be considered the lowest power setting on a microscope to get a good look at spores? Would 200x be enough? reason I am asking I found a cheap USB microscope and that is the higest I can go with it..I have another that will do 1200x but it needs a good cleaning..I am sorry if this is the wrong fourm to post this in..If so please move it to the proper one...

Thanks in advance!

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Re: Lowest power on microscope? [Re: VividDreams]
    #2015819 -

200x should be fine -- spores are really big and they're dark, so you don't even need to stain them (if they're coming from a sporeprint.)

Depends on how big you want them to look.

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Micro


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Re: Lowest power on microscope? [Re: micro]
    #2015964 -

What kind of power do you need to measure?

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Re: Lowest power on microscope? [Re: YidakiMan]
    #2016801 -

200x should be enough for measuring. You'll need more than that to view internal structures and spore ornamentation.

These are cubensis spores at 675x:

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Re: Lowest power on microscope? [Re: zeronio]
    #2017083 -

I mostly use mine to look to see how much bacteria is on the spores before use. I'm not sure you can see those at less than 400X. 100X gives you a nice view of the mycelia and is plenty to see the clamp connections.


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Re: Lowest power on microscope? [Re: RogerRabbit]
    #2017122 -

I have this ancient 100X-600X russian microscope, is it worth cleaning
up and using? I assumed it was too small.


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Re: Lowest power on microscope? [Re: Paid]
    #2017224 -

No, it's not too small. I think you hardly need more than that.

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Re: Lowest power on microscope? [Re: zeronio]
    #2017240 -

Yay, I have a microscope then :]
I better spend some time cleaning it up, any tips on
lens care, cleaning?


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Re: Lowest power on microscope? [Re: Paid]
    #2017289 -

Oh, I just saw my first spores :]
Amanita Muscaria, sort of lemon shaped, with bubbles inside :]
Now how the hell do i measure them lol ?


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Re: Lowest power on microscope? [Re: Paid]
    #2017364 -

The best thing for cleaning it is the air spray.

You'll need a stage micrometer or something of known micrometer size. (Don't ask me what could that be) Then take a picture of micrometer and then of the spores at the same magnification.
I can measure them for you then...

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Re: Lowest power on microscope? [Re: zeronio]
    #2017371 -

What could that be ? :tongue:

























lol, I'll dig about, see what i can come up with :]


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Re: Lowest power on microscope? [Re: Paid]
    #2017437 -

Make sure to clean the lenses with lens paper, and kodak sells a good cleaning solution.

If there's still crap stuck to the glass after a good cleaning, cleaning *very carefully* with xylene can do wonders, but don't get any outside of the glass area or you'll melt the glue and the lens will fall apart.

It's a lot easier to do all this under a dissection microscope, if you can.

Usually a hemacytometer is used to measure things; it sucks ni rulers go down to that size :wink:

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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