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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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The Prince rises!
    #17405111 -

first off I would like to thank the Shroomery community as a whole for all the information. Without you none of this would be possible.

This project was a long time in the making. Back in march i collected a beatiful clean Agaricus augustus. it was so beautiful i wanted to clone it and try an indoor grow.
so i started out by using the Grocery Store Agar Tek to make ghetto petri dishes. after a few transfers i had clean culture which i then allowed to colonize the dish. by june the dish had fully been colonized, and from there i used a WBS tek and transferred some agar into a few dishes.
after suffering a few loss jars due to contams, i was left with (2) 1/2pint jars of spawn. and in oct i spawned the pint of wbs to a pasturized mix of coco coir, verm, with a little bit of straw bedding at about a 45%/45%/10% ratio. this was allowed to colonize till dec 1. on dec 1 my substrate was introduced to fruiting condition and within the week pins could be seen. at this point i still have buttons but i will be taking pic until i get a spore print.
and now some pics
12/12


12/15


12/16


--------------------
Grocery Store Agar Tek
damion5050's coir Tek
A. augustus grow

"I see the mycelium as the Earth's natural Internet, a consciousness with which we might be able to communicate... Because these externalized neurological nets sense any impression upon them, from footsteps to falling tree branches, they could relay enormous amounts of data regarding the movements of all organisms through the landscape."
Paul Stamets

Extras: Filter Print Post Top
Re: The Prince rises! [Re: fungal_alchemist]
    #17405714 -

Very interesting. Did you encounter any issue with agar? I cloned a specimen that is painfully slow, both on PDA and in MEA.


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Re: The Prince rises! [Re: atricoz]
    #17409438 -

Nice work! I've not tried a. Augustus but it looks yummy. :biggrin:

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Re: The Prince rises! [Re: fungal_alchemist]
    #17409603 -

Nice work.  Take care of your culture.  I haven't been able to get even the freshest spores directly from cultivated fruits to germinate on agar.  Even tricks that work for Agaricus bisporus have failed me.  If you figure it out, be sure to post your method.

My Prince Cultivations


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting Sales Towards Basic Research and Species Identification :amanitajar:

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Re: The Prince rises! [Re: Workman]
    #17418880 -

Workman has spoken!

Nice work OP. 

Thanks for sharing.

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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Re: The Prince rises! [Re: Workman]
    #17426775 -

Workman said:
Nice work.  Take care of your culture.  I haven't been able to get even the freshest spores directly from cultivated fruits to germinate on agar.  Even tricks that work for Agaricus bisporus have failed me.  If you figure it out, be sure to post your method.

My Prince Cultivations


This is very interesting! Here's to hoping I can revive my old culture

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Re: The Prince rises! [Re: Kyuule]
    #17429716 -

Thanks for the replies all
@atricoz
I used mea and didnt have issues with my tissue samples taking other than it was weeks before anything happened. Once there was mycilum jumping to the agar there was a growth spurt and it ran the plate. I didn't see problems until i moved to grain. I assumed i didn't sterilized my grains well in those jars or just random spores getting into my glove box during transfers but even the jars that took there was a few weeks before the mycilum jumped. My advice is just watch it for a day or so to make sure there is no contams then forget about it for a month or so and then take a look at it.
@deadmandave
Thanks! Im Gunna cook them up in a day or so should be tasty
@workman
I wish i had some trick or tip to give but its all in the culture i think. I just got lucky. But believe me im taking good care of it!
@javadog
Np thanks for viewing.
@kyuule
Thanks and good luck with your culture.
Here are a few more pic for everyone
12/18

12/19


--------------------
Grocery Store Agar Tek
damion5050's coir Tek
A. augustus grow

"I see the mycelium as the Earth's natural Internet, a consciousness with which we might be able to communicate... Because these externalized neurological nets sense any impression upon them, from footsteps to falling tree branches, they could relay enormous amounts of data regarding the movements of all organisms through the landscape."
Paul Stamets

Extras: Filter Print Post Top
Re: The Prince rises! [Re: fungal_alchemist]
    #17430162 -

Wow.  Those are really taking off!


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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Re: The Prince rises! [Re: Javadog]
    #17431392 -

Very cool! Are you going to take prints from those?

They are beautiful looking mushrooms. :wink:


--------------------
_-_--->My Trade List<---_-_

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Re: The Prince rises! [Re: Humbled]
    #17431583 -

@javadog
Yea they blew up the last few days
@psilocelium
I will take at least one print in hopes that i can reproduce this starting from spores. Who knows maybe i get lucky again.

I will post a pic of the spores print after i let the spores drop a lil while longer.


--------------------
Grocery Store Agar Tek
damion5050's coir Tek
A. augustus grow

"I see the mycelium as the Earth's natural Internet, a consciousness with which we might be able to communicate... Because these externalized neurological nets sense any impression upon them, from footsteps to falling tree branches, they could relay enormous amounts of data regarding the movements of all organisms through the landscape."
Paul Stamets

Extras: Filter Print Post Top
Re: The Prince rises! [Re: fungal_alchemist]
    #17431691 -

Cloning is what I did and I also experienced the very long lag times when transferring to different media.  The mycelium takes an extraordinarily long time to adapt to a different substrate.  But when it finally figures it out, it grows and fruits easily.  Germinating spores is my only problem.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting Sales Towards Basic Research and Species Identification :amanitajar:

Extras: Filter Print Post Top
Re: The Prince rises! [Re: Workman]
    #17431963 -

:omg: this is an awesome grow!! being one of my favorite of all edible mushrooms , i am very happy to see your success. :congrats:
way to go!!


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Re: The Prince rises! [Re: psylosymonreturns]
    #17440733 -

Interesting grow; I look forward to seeing what you can do with the spores and/or tissue cloning.


--------------------
_-_--->My Trade List<---_-_

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Re: The Prince rises! [Re: Workman]
    #18135316 -

Firstly, amazing grow, congratulations.
would love to get a culture of this.


Workman said:
Nice work.  Take care of your culture.  I haven't been able to get even the freshest spores directly from cultivated fruits to germinate on agar.  Even tricks that work for Agaricus bisporus have failed me.  If you figure it out, be sure to post your method.

My Prince Cultivations


Had same issue, with Augustus and with wild Agaricus I collected.
The trick to getting fast and furious germination was organic matter, take the same grains you use as spawn, grind to powder, mix with water until very runny.
PC plates and inoculate. Had full plates within 10 days in ghetto grain agar, the bonus is also they now colonize grain Spawn rapidly due to already recognizing it.

In one plate I neglected there were rhizomorphs nearly 1/8" thick.


--------------------
¯\_(ツ)_/¯

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Re: The Prince rises! [Re: Tmethyl]
    #18135380 -

Tmethyl said:
Firstly, amazing grow, congratulations.
would love to get a culture of this.


Workman said:
Nice work.  Take care of your culture.  I haven't been able to get even the freshest spores directly from cultivated fruits to germinate on agar.  Even tricks that work for Agaricus bisporus have failed me.  If you figure it out, be sure to post your method.

My Prince Cultivations


Had same issue, with Augustus and with wild Agaricus I collected.
The trick to getting fast and furious germination was organic matter, take the same grains you use as spawn, grind to powder, mix with water until very runny.
PC plates and inoculate. Had full plates within 10 days in ghetto grain agar, the bonus is also they now colonize grain Spawn rapidly due to already recognizing it.

In one plate I neglected there were rhizomorphs nearly 1/8" thick.


awesome idea man!! i germinated my auagustus spores with a woodchip water MEA. but i like your plan better.

i just got some prince on grain the other day actually. no leap off yet.


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Re: The Prince rises! [Re: psylosymonreturns]
    #18141738 -

Don't know how I missed this thread.  What a result and from a small/shallow substrate!!  How did they taste?!

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Re: The Prince rises! [Re: Tmethyl]
    #18142129 -

Tmethyl said:
Firstly, amazing grow, congratulations.
would love to get a culture of this.


Workman said:
Nice work.  Take care of your culture.  I haven't been able to get even the freshest spores directly from cultivated fruits to germinate on agar.  Even tricks that work for Agaricus bisporus have failed me.  If you figure it out, be sure to post your method.

My Prince Cultivations


Had same issue, with Augustus and with wild Agaricus I collected.
The trick to getting fast and furious germination was organic matter, take the same grains you use as spawn, grind to powder, mix with water until very runny.
PC plates and inoculate. Had full plates within 10 days in ghetto grain agar, the bonus is also they now colonize grain Spawn rapidly due to already recognizing it.

In one plate I neglected there were rhizomorphs nearly 1/8" thick.


Very interesting idea. Will need to try that. I'll keep you posted on a culture as soon as i get enough to work with. Its been taking forever to get it growing again and every attempt to get a glc or lc have shown no growth in test jars after 3 months. Starting new plates in hopes on catching it growing. My original plate is shrinking its so old now. I would love to hear any more tips or tricks with this species.
Btw they tasted great! Made a mushroom bisque using those princes and some candy caps. Turned out to be a big hit with the family


--------------------
Grocery Store Agar Tek
damion5050's coir Tek
A. augustus grow

"I see the mycelium as the Earth's natural Internet, a consciousness with which we might be able to communicate... Because these externalized neurological nets sense any impression upon them, from footsteps to falling tree branches, they could relay enormous amounts of data regarding the movements of all organisms through the landscape."
Paul Stamets

Edited by fungal_alchemist (04/20/13 10:09 PM)

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Re: The Prince rises! [Re: fungal_alchemist]
    #18142202 -

Ya they are outstanding eh!!!

I am thinking about doing an LC since ive been having great success using LC for other slower species . I really love A augustus!


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Re: The Prince rises! [Re: psylosymonreturns]
    #18157736 -

That looks like really good biological efficiency, the msurhooms are almost boigger than the substrate! That strain bye the way is really valuable, make sure you take ggood care of it .

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Re: The Prince rises! [Re: Lennybernadino]
    #18157767 -

Hey what are your fruiting temps, and humidity?

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