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Offlinekeyzar
journeyman
Registered: 04/03/00
Posts: 56
Last seen: 21 years, 11 months
Re: Honey Tek, by hippie....has anyone ever done it with success?
    #14339 - 04/25/00 07:56 PM (23 years, 9 months ago)

the first time i did it it worked out gr8 the second time i had contam problems big time but i think its a good tek if your work area and incubation chamber is clean
sounds like you did everything right

[This message has been edited by keyzar (edited April 25, 2000).]



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OfflinePappaKaps
enthusiast
Registered: 03/29/00
Posts: 315
Last seen: 22 years, 9 months
Re: Honey Tek, by hippie....has anyone ever done it with success? [Re: keyzar]
    #14341 - 04/25/00 08:01 PM (23 years, 9 months ago)

So, when is it easiest to get contaminated??? While your inoculating the honey water jar? Or from the honey jar into your syringes??


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Invisibletokinshitload
t
Registered: 02/13/00
Posts: 198
Re: Honey Tek, by hippie....has anyone ever done it with success? [Re: keyzar]
    #14342 - 04/25/00 08:22 PM (23 years, 9 months ago)

i had no problem,got 12 good fruiting cakes,off one cc spores,cakes were done in 2 weeks cause i was able to put 6 cc myclium water in each jar.........


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InvisibleLinkA
Intrinsically No Good

Registered: 07/28/99
Posts: 2,314
Loc: Lost Woods, Hyrule
Re: Honey Tek, by hippie....has anyone ever done it with success? [Re: keyzar]
    #14343 - 04/25/00 09:58 PM (23 years, 9 months ago)

This may belong in its own topic, but oh well. What if you took a piece of a colonized cake, chopped it up like in funkyballoon's tissue culter tek, and put it with some h2o2 to kill some of the nasties, and threw that in with the honey water. that way you wouldn't have to wait for the spores to germinate, and contam rate should theoretically go down. any thought? critisisms? i think i may try it out

Link

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Wonder had gone away, and he had forgotten that all life is only a set of pictures in the brain, among which there is no difference betwixt those born of real things and those born of inward dreamings, and no cause to value the one above the other.  -- H.P. Lovecraft "The Silver Key"


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Invisibletokinshitload
t
Registered: 02/13/00
Posts: 198
Re: Honey Tek, by hippie....has anyone ever done it with success? [Re: keyzar]
    #14344 - 04/26/00 10:38 AM (23 years, 9 months ago)

ill give that a try link,sounds good if you already have a colinised cake to hack at.........


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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
Re: Honey Tek, by hippie....has anyone ever done it with success? [Re: keyzar]
    #14345 - 04/26/00 05:29 AM (23 years, 9 months ago)

that way works too of course. but that is cloning. after a clone or 2, the myc. will lose vigor and you must re-start from spores anyway.

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OfflinePhilosoPossum
member

Registered: 02/16/00
Posts: 131
Loc: Toronto, Canada
Last seen: 20 years, 2 months
Re: Honey Tek, by hippie....has anyone ever done it with success? [Re: keyzar]
    #14346 - 04/26/00 12:41 PM (23 years, 9 months ago)

I'm still new to growing, but I gave the honey tek a shot, and lo and behold, it worked. I also tried the mushroom-in-a-blender tek, and it also worked! (I didn't think I'd get them right on the first try...) The jars took a week to start showing any growth, but they're doing very well now after 3 weeks.

However one small problem is that the solution I suck into my syringe seems to be uneven in its mycelium distribution. Not very homogeneous. I say this because even though all my jars are now growing, about 30% of my innoculation sites completely failed (using about 2-3 ccs per site). This is creating some annoying uneven growth in some jars.

So... before filling the syringe, with the honey tek, do you first vigorously shake up the jar? And with the mushroom-in-a-blender tek, once the h202 is added, the mycelium floats to the surface. This makes it difficult to suck up. Any suggestions?



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OfflinePappaKaps
enthusiast
Registered: 03/29/00
Posts: 315
Last seen: 22 years, 9 months
Re: Honey Tek, by hippie....has anyone ever done it with success? [Re: keyzar]
    #14347 - 04/26/00 12:47 PM (23 years, 9 months ago)

I just inoculated my honey water........it seems there is alot of honey left on the bottom, should I shake it up. And how exactly does this tek work, anyone know?


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InvisibleHippie3
mycotopiate
 User Gallery

Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
Re: Honey Tek, by hippie....has anyone ever done it with success? [Re: keyzar]
    #14348 - 04/26/00 06:25 PM (23 years, 9 months ago)

it may help to include a penny in the jar when you prepare it. this might help to break up the mycellial clumps.
a few seconds in the blender will do the same.
i use filter disks on the jars instead of lids then pour off most of the water, leaving a very concentrated solution.
a few drops will work 100%.
otherwise, just try to suck up the myc. while leaving most of the water behind.
this tek works because it essential multiplies a few hundred cells (spores) into millions of cells.
this is not cloning,
it is true multi-spore germination.
the jar/culture will die after eating the sugar but one can just inject a syringe full into fresh jars of honey water to extend lifespan.

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