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OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
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Using a .5 - 1% bleach solution to sterilize substrate?
    #1372726 - 03/13/03 01:08 PM (15 years, 3 months ago)

This was originally posted on the advanced cult board, but I deleted that copy to effectively move it here. I wasn't getting any resposes over there.

Could one use a .5 - 1% bleach solution to chemically sterilize a dry substrate, such as dried manure? One could spray the container and dry substrate with the solution and let it sit for a day or two, or even heat it to speed up the process. After this, one could spray distilled water on the substrate until it is wet, drain it off, and introduce spawn. I doubt there would be enough bleach left to seriously disrupt colonization, especially if you first soak your cakes in sterile water before introducing the mycelium to the substrate. This will subject the mycelia to a smaller influx of bleach in solution upon introduction.

My friend wants to try it, but doesn't want to waste his time if someone here sees a major flaw he does not!

If this works, I can already see it being used in bulk teks, were sterilization becomes increasingly difficult.


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Offlineamyloid
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Re: Using a .5 - 1% bleach solution to sterilize substrate? [Re: BigJohnson]
    #1372970 - 03/13/03 02:33 PM (15 years, 3 months ago)

i dont know wether it would work, but i would be concerned about the mushies possibly becoming a residual bleach trash dump for the mycellium and possible effects of bleach on alkaloids/structure... i would just stick with heat sterilization, if the bleach method worked i think someone would have mentioned it in print.

-doc


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OfflineGWAR
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Re: Using a .5 - 1% bleach solution to sterilize substrate? [Re: amyloid]
    #1372976 - 03/13/03 02:35 PM (15 years, 3 months ago)

hippi3 used bleach to kill contams.


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OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
Posts: 716
Loc: The Yard
Last seen: 14 years, 11 months
Re: Using a .5 - 1% bleach solution to sterilize substrate? [Re: GWAR]
    #1372984 - 03/13/03 02:38 PM (15 years, 3 months ago)

Quote:

hippi3 used bleach to kill contams.




That's where I came up with idea.


--------------------
Should the US relegalize drugs?

http://www.lp.org/issues/relegalize.html


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OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
Posts: 716
Loc: The Yard
Last seen: 14 years, 11 months
Re: Using a .5 - 1% bleach solution to sterilize substrate? [Re: BigJohnson]
    #1373042 - 03/13/03 02:59 PM (15 years, 3 months ago)



--------------------
Should the US relegalize drugs?

http://www.lp.org/issues/relegalize.html


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OfflineNorthernsoul
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Registered: 11/17/01
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Re: Using a .5 - 1% bleach solution to sterilize substrate? [Re: BigJohnson]
    #1373051 - 03/13/03 03:01 PM (15 years, 3 months ago)

Sorry, couldnt tell ya, never heard of doing this...good luck, let us know how it goes :cool:


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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