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Invisiblephrozendata
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have any of you tried the Neimad Method?
    #13538 - 04/21/00 09:43 PM (16 years, 10 months ago)

have any of you tried the Neimad Method which suggest adding the spores to the water used to mix that cakes (ie - 2/3 cup of vermiculite, 1/4 cup brown rice flower and 1/2 cup spore water solution).. this methond is suspose to be a quick Spore Germination, and does not have to have the substrate pressure cooked or steam sterlized... anybody?

*edit*
I have just noticed this is a Tek --
High Fun Guy's 10-step -=Quicky Colinization=- -- Tek
*edit*

[This message has been edited by phrozendata (edited April 21, 2000).]



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Invisiblevivid
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Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #13539 - 04/22/00 03:10 AM (16 years, 10 months ago)

uhmm that guys obviously a dumbass.. because you have to sterilize the substrate thus KILLING the spoers in the spore water.. :shakes head:.. So don't bother trying it unless you want to waste perfectly good spores.

vivivd



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Invisiblephrozendata
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Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #13540 - 04/22/00 10:58 AM (16 years, 10 months ago)

ovbiously you dont get it, you dont sterlize the substrate, the whole cake should be colonized in 6 to 10 days, again has anybody here used it to sucess?


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"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
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Invisibleneverwhere
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Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #13541 - 04/27/00 10:17 PM (16 years, 9 months ago)

obviously vivid is right.....wouldnt not sterilizing the substrate bring on the contams?? phrozen, where did u find this? are you going to try it? (sorry to bring up an old post i was reading up on mycelium and saw this)


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Invisiblephrozendata
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Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #13542 - 04/28/00 01:00 AM (16 years, 9 months ago)

I found it in the Grow/Find section, dont you guys ever read? I dont think you totally understand what it is saying, you add spores to the water that you use to mix the substrate, and you do NOT sterlize the substance (this is the etchy part) because the whole cake should be colonized within 6 to 10 days, rather than just starting in 6 to 10 days, so ovbiously the speed of the growth is going to help the fight againsts contams. I have never tried this, and was asking if any of you have, because like I said, and like you are asking, not sterlizing the substrate is risky, but if the cake is colonized in 6 to 10 days it doesnt not have a huge chanch to get contaminated. Anyways, you still have to somewhat sterlize the substrate, you cook the BRF and vermiculite in the oven, and mix using the low doller sterile tek. I will probably try this next time.


--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


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InvisibleHippie3
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Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #13543 - 04/28/00 08:57 AM (16 years, 9 months ago)

it does NOT work.
bacteria are far faster than spores.
you might have a slim chance of success if you used a massive amount of mycellial slurry mixed with peroxide.

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OfflineoOjonahOo
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Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #13544 - 04/28/00 11:27 AM (16 years, 9 months ago)

The idea is to PRE-sterilize everything. All the substrate materials : rice flour, vermiculite, and water. The water is boiled, cooled and innoculated, then that is used to mix the substrate in sterile conditions. It is not a given that contamination will show up, the procedure just sterilizes before mixing rather than after. Attention must be paid to sterility when mixing the substrate, rather than after pressure cooker like in other teks. There is supposedly a reduced chance of conatinants because the it will colonize so quickly.


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Anonymous

Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #13545 - 05/01/00 10:36 PM (16 years, 9 months ago)

Let me tell you, this method does NOT work. I tried it once a year or so ago... Bad idea unless youre working completey under a HEPA flow hood or something similar.... It's just IMPOSSIBLE to acheive the level of sterility nexessary... However, look into Jaff's H202PF substrate tek... That one is basically the same only with H202 and mycelium water.. Worked AMAZINGLY for me the two times I tried it.... :-) Thanks again JafF if you ever see this!


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Anonymous

Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #13546 - 05/01/00 11:59 PM (16 years, 9 months ago)

Why the hell would anybody want to try that technique when there are many other teks which are far safer than that. Why take the risk of not sterilizing? For shit's sake have some patience and let colonization happen the right way.

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Anonymous

Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #13547 - 05/02/00 03:45 PM (16 years, 9 months ago)

I think there are many "right" ways... No one way is right, and that is the fun of mycology :smile: Although, I must admit, this method does just seem WRONG.


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OfflineDead Shaman
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Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #313669 - 05/10/01 02:49 AM (15 years, 9 months ago)

Using the Neimad method I have acheived fully colonized 1 pint cakes in about two weeks. Worked awesome no contamination. My question is will i be able to get the cakes out of the jars. Due to not boiling the substrate in the jars the cakes did not harden or shrink. And if the cakes come out will the mycelium be enough to hold them together or will they crumble? Also I had planned on trying to grow them invetro, but is that feesible if the cakes havnt shrunk, also I didn't colonize the cakes in light, does that really matter.

"Or at least that's what the monkey told me."


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InvisibleMyco-420
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Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #313900 - 05/10/01 12:54 PM (15 years, 9 months ago)

THis is rediculous,,,,,, I have trouble keeping molds and bacterias out of my shit even when I sterilize... WHo ever wrote the tek was is some kind of class 100 clean room.

Someone should email me a bowl.


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OfflineDarK_SavioR
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Re: have any of you tried the Neimad Method? [Re: phrozendata]
    #313948 - 05/10/01 02:03 PM (15 years, 9 months ago)

hmm, I get the theory behind this but it doesn't look like whoever came up with this was including all the factors... I get what its implying is that the mycelium will colonize so fast that it will be able to defend itself from the contams... but, I doubt it'd work as successfull as almost all other method out there, heh. What would make you think the contams aren't gonna grow as fast or faster than the mycelium? I recommend sticking with the pftek or something else. Even if this method might be faster the percentage of success wouldn't be worth it and in the long run it'd actually be quicker to sterilize and then innoculate for the fact that you're gonna be bringing in more shrooms this way and not losing the majority of your cakes to contams.

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