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Amazon Shop: ½ Pint Jars, Agar, Malt Extract, Petri Dish

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Offlineindicaz
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AGAR TEK....
    #1209030 - 01/11/03 10:50 AM (14 years, 4 months ago)

OK someone please help...

I have 2 types of agar

Malt Extract agar - wheat

and

Bacteriological Agar

Whats the difference and does anyone have a good preperation TEK for agar

Using a 40 ounce beer bottle.......... the instructions from sporeworks say

40 grams per liter of water....... So what would I use roughly on a 40 ounce bottle?

Thanks




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OfflineAnnoA
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Re: AGAR TEK.... [Re: indicaz]
    #1209185 - 01/11/03 11:48 AM (14 years, 4 months ago)

40 ounces of water is 1.134 liter.


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Offlineindicaz
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Re: AGAR TEK.... [Re: Anno]
    #1209235 - 01/11/03 12:12 PM (14 years, 4 months ago)

any difference between both agar's?

can i mix 50/50 of each?

thankx anno


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OfflineRaadt
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Re: AGAR TEK.... [Re: indicaz]
    #1209541 - 01/11/03 02:43 PM (14 years, 4 months ago)

the bac agar has no nutrients, you'll need to add them. There's recipe's in the FAQ. The other is a pre-mix, which contains nutes. You use it at the suggested mixture.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Invisiblecheesenoonions
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Re: AGAR TEK.... [Re: indicaz]
    #1211075 - 01/12/03 04:45 AM (14 years, 4 months ago)

This is all in the FAQ. I'm surprised you didn't get it yelled at you.


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OfflineAnnoA
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Re: AGAR TEK.... [Re: cheesenoonions]
    #1211090 - 01/12/03 04:58 AM (14 years, 4 months ago)

We don?t yell in this forum, we are not OTD.


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Invisiblecheesenoonions
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Re: AGAR TEK.... [Re: Anno]
    #1211122 - 01/12/03 05:52 AM (14 years, 4 months ago)

this isn't yelling ?


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Invisibleshaggymane
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Re: AGAR TEK.... [Re: cheesenoonions]
    #1211133 - 01/12/03 06:04 AM (14 years, 4 months ago)

maybe thats yelling-my beer tek I use is 1 beer 3 water 20g agar agar-easy bob's your boyfriend


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OfflineAnnoA
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Re: AGAR TEK.... [Re: cheesenoonions]
    #1211138 - 01/12/03 06:07 AM (14 years, 4 months ago)

Well, you?re right, sometimes we do, but we shouldn?t be.


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Invisiblecheesenoonions
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Re: AGAR TEK.... [Re: indicaz]
    #1211206 - 01/12/03 07:10 AM (14 years, 4 months ago)

A couple of things I would suggest when using agar, if you are going to use a 40 ounce container, use only half of the container's capacity. If you fill it to the top, and PC it it will boil over. So prepare only about 500mL or 20 ounces. That is more than enough. Pressure cook the media for as little time as possible 20-25 mins works for me. If you are going to add antibiotics or peroxide after you PC, do it when the agar has cooled down. Pour into plates when the agar is very cooled down. This way there is less condensation. If you do not have petri dishes, use jars. Keep in mind that agar dries out. I like to keep a plate that I do nothing with and store it in the incubater with the other dishes. This is a negative control. If i get contams in my dishes AND the neg control I need to pressure cook longer or pour faster and improve sterile technique. Here are some pics of my own strain isolated on peroxidated MEA. i used the plate seperated into 4 sections so that i don't have to use a whole butt load of plates.






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Amazon Shop: ½ Pint Jars, Agar, Malt Extract, Petri Dish

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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