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Offlineragadinks
MrBeatle
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Proper temperature and duration for sterilizing agar?
    #2083908 - 11/08/03 02:29 PM (13 years, 5 months ago)

When reading the various agar tek's the time for sterilizing agar differs from "not more than 15 minutes" to "about 40 minutes" at 15 psi. Some say that when the sterilization temp exceeds 250 ?F ( 121 ?C ) the sugar will caramelize the media and ruin it. I have tried both - sterilizing for 20 and for 40 minutes and the agar seemed to be ok both times. What temperature/duration values are correct now?


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OfflineChromeCrow
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Re: Proper temperature and duration for sterilizing agar? [Re: ragadinks]
    #2083919 - 11/08/03 02:33 PM (13 years, 5 months ago)

i use 30-40 mins at 15 psi with no problems, or caramelizing... im not sure if this is text-book, but i havent had an issue with these times/temps.


hope this helps a lil bit
CC


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Offlineragadinks
MrBeatle
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Re: Proper temperature and duration for sterilizing agar? [Re: ChromeCrow]
    #2083952 - 11/08/03 02:48 PM (13 years, 5 months ago)

That worked also for me ...


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Invisiblemicro
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Re: Proper temperature and duration for sterilizing agar? [Re: ragadinks]
    #2083995 - 11/08/03 03:02 PM (13 years, 5 months ago)

I've read 20 min. in a lot of places, too, but I think 15 is better. It comes out a lot lighter when you do 15, and it's a liquid so it should sterilize pretty quickly.

I've never had a problem at 15. Just wait for the pressure to go up to 15 psi, of course, before counting. 20 should be ok too, though.

--
Micro


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Offlinewalk
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Re: Proper temperature and duration for sterilizing agar? [Re: micro]
    #2084702 - 11/08/03 07:30 PM (13 years, 5 months ago)

I pc for 15 at 15.
I know a guy who always uses a microwave and has fine results.


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Offlineragadinks
MrBeatle
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Re: Proper temperature and duration for sterilizing agar? [Re: walk]
    #2084774 - 11/08/03 08:13 PM (13 years, 5 months ago)

I know a guy who always uses a microwave and has fine results.

How long did he sterilize the agar in the microwave ?


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-> It Is Not Important WHO Is Right But WHAT Is Right <-


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Offlineragadinks
MrBeatle
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Re: Proper temperature and duration for sterilizing agar? [Re: walk]
    #2089365 - 11/10/03 02:41 PM (13 years, 5 months ago)

I have tried now for 15 minutes instead of 40 and the color is really much lighter.


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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