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Mushrooms, Mycology and Psychedelics > The Ethnobotanical Garden
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GA3 for Germination - An Experiment for Circumstantial Data!
    #10562777 -

Recently I acquired some GA3 for the purpose of experimenting with my plants.  I began with this experiment in seed germination:


Experimentation Table

I set up a 7x3 grid with the X axis as plant seed type, and the Y axis as increasing concentrations of GA3 (50 PPM, 100 PPM, 200 PPM).

Sets of seeds were placed in 1mL of each solution. The seed species and number per set are as follows:
1.) Two (2) Scarified Psychotria virdis
2.) Two (2) Undamaged Psychotria virdis
3.) Four (4) Nymphaea caerulea
4.) Four (4) Coffee arabica 'Catimor'
5.) One (1) Ziziphus zizyphus
6.) One (1) Scarified Musa sikkimensis
7.) Four (4) Peganum harmala


Grid set-up.

Seeds soaked for 24 hours before removal, and were potted in Pro-Mix potting soil, with the exception of Nymphaea caerulea, the treatment of which can be found here:
http://www.shroomery.org/forums/showflat.php/Number/10562563

Germination dates for seedlings will be determined to see if there is correspondence with use of GA3.  Each seed type has an untreated control group that was placed in moist Pro-Mix. 


Some of the potted seedlings.

Additional Notes:
* GA3 must be dissolved in alcohol before diluting.  I used a squirt of denatured methanol/ethanol they sold in the painting section of my hardware store.
*The 'Test Tubes' are actually the things they cap cut flowers with when you order them online.  I had a bunch saved, thinking they would be useful for something like this. I rinsed them with distilled water before using them here. 
* 50, 100, and 200 PPM are fairly low concentrations. Different seeds apparently respond to different concentrations. If I were to try this again, I'd probably add a 500 and 1000 PPM column. 
* I figured I would try scarrifying the Psychotria virdis to see if that had any effect.  I wasn't sure the hormone would penetrate the seed-coat otherwise. We shall see. 
*  Coffee arabica 'Catimor' is a cross of Timor an Catura coffees that it disease resistant, absurdly productive, and is apparently quite poor in taste, which is fine, because I prefer bitter sludgy coffee. 
*Ziziphus zizyphus is the Chinese Jujube.  I want these in my yard.
*Musa sikkimensis is the Darjeeling Banana, a variety that is known to be extremely cold hardy, a trait that is desirable in Zone 6. The tops will die when it gets cold, but the root will continue to live, and put out trees every spring.
*By no means are the results of this experiment to be comprehensive, or even conclusive.  Circumstantial Data is exactly what this will yield, but I hope that whatever happens helps guide other forum members with their own experiments.
*This thread will be updated as stuff happens.


--------------------
The Plant Mage Guild

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Mushrooms, Mycology and Psychedelics > The Ethnobotanical Garden
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