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Mushrooms, Mycology and Psychedelics > The Ethnobotanical Garden
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My GA3 came in today. Bring your experiment suggestions here.
    #10537395 -

I just got a couple vials of 90% GA3 (Gibberellic acid).  I think it will be fun experiment fodder for this summer. I have some P. virdis seeds arriving soon, so I know I'm going to try getting faster germination times from them.  That's... really the only thing I had in mind to test, although I'm sure some more ideas will come to me.  That's also why I'm making this thread. If you have anything in mind that you want to see tested with GA3, please post your ideas here.  I probably won't do all of them, but I might choose some ones that I like and see what happens.  As far as materials, I have plenty of hypodermic needles, syringes, and other miscellaneous supplies around (though I don't have a set-up for tissue culture).

Ungerminated Seeds:
Chinese Jujube
Dwarf Papaya
Coffee arabica "Catimor"
Darjeeling Banana
Japanese Rasin Tree
Argyreia nervosa
Peganum harmala
Mimosa hostilis
Panax quinquefolius
Lophorphora williamsii
Nymphaea caerulea
Psychotria virdis

Seedlings:
Passiflora edulis (haha, they finally came up, and damn near close to 100% germ, even after all the abuse)
Argyreia nervosa
Mimosa hostilis
Borago officinalis (Borage)
Alpine Strawberries

Established Plants:
Some ornamental Datura cultivar
Passiflora incarnata
Tomatoes
Cucumbers
Beets
Squash/Zuchinni
Watermelons
A bunch of other stuff...

ANYWAY, if you've got suggestions for GA3 experiments with any of those plants, or something else you want to see, post 'em here.  If the virdis experiment works, I'd like to see if I could coax better germ rates out of other stuff that is notoriously hard to sprout.

EDIT: Changed typo in Title. to day --> today


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The Plant Mage Guild

Edited by Horticulture (06/19/09 04:40 PM)

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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: Horticulture]
    #10537405 -

Also note that I'm going to be away to the weekend, so I won't be around or replying until Sunday.


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The Plant Mage Guild

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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: Horticulture]
    #10538846 -

For the ungerminated seeds - not all of them will need the GA.

I'd like to see if GA works for stalled cactus, do you have any to try? Would it boost seedlings growth?


FH

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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: felixhigh]
    #10539235 -

Feminized pot seeds maybe?

EG


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MY GROW CLOSET- update 06/17/09

MY IDIOT PROOF OPIUM POPPY TEA TEK

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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: Horticulture]
    #10541396 -

there was a guy on another forum that used GA3 in combination with BAP, he made a solution in a sprayer and misted his loph grafts and some other cacti with it...
they pupped from every aerole lol and then the pups pupped and pupped and pupped... like a "witches broom" effect...

i guess it was the BAP that did it as latter experiments proved that only the BAP was required.


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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: Dr. uarewotueat]
    #10555908 -

Oh snaps... I'm about to mix this shit.  Wish me luck guys. I'll snap some pictures when my setup is complete. 

Right now my cactus collection is not so big, but once I start grafting lophs, I'll see what sort of nonsense I can induce.


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The Plant Mage Guild

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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: Horticulture]
    #10556147 -

I have a vial, use it sparingly I was messing around and sprayed a good deal on a lettuce plant about 4 inches high, in a week it's leaves grew extremely long and thin and then it just died. A tomato receiving the same treatment did very nicely. Different plants have different tolerances.

I plan on soaking my San Pedro seeds in 2000ppm GA3 for 24 hours to help them germinate as I haven't had much luck recently...


EDIT: Dr. uarewotueat do you have a reference for that post or how much was used?


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"Life without freedom is not life... freedom is life."

Edited by crazyboy25 (06/22/09 10:34 PM)

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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: crazyboy25]
    #10557161 -

2,000 PPM?! That seems absurd. 

I set up a grid where I made vials with 50 PPM, 100 PPM, and 200 PPM, and am trying different seeds in them to see how the germ rates compare to unsoaked seeds.  I've never heard of anyone using a ratio as high as 2000 for germinating (or anything for that matter).  I would not be surprised if that level would ensure those seeds would never germinate.  Then again, I'm certainly no veteran of this germination technique.


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The Plant Mage Guild

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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: Horticulture]
    #10558645 -

Horticulture said:
2,000 PPM?! That seems absurd. 

I set up a grid where I made vials with 50 PPM, 100 PPM, and 200 PPM, and am trying different seeds in them to see how the germ rates compare to unsoaked seeds.  I've never heard of anyone using a ratio as high as 2000 for germinating (or anything for that matter).  I would not be surprised if that level would ensure those seeds would never germinate.  Then again, I'm certainly no veteran of this germination technique.


http://www.crfg.org/tidbits/gibberellic.html

Check out the chart at the bottom.


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"Life without freedom is not life... freedom is life."

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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: crazyboy25]
    #10558915 -

crazyboy25 said:
Horticulture said:
2,000 PPM?! That seems absurd. 

I set up a grid where I made vials with 50 PPM, 100 PPM, and 200 PPM, and am trying different seeds in them to see how the germ rates compare to unsoaked seeds.  I've never heard of anyone using a ratio as high as 2000 for germinating (or anything for that matter).  I would not be surprised if that level would ensure those seeds would never germinate.  Then again, I'm certainly no veteran of this germination technique.


http://www.crfg.org/tidbits/gibberellic.html

Check out the chart at the bottom.



Hmmm... interesting.  I based my relatively low numbers off a few different studies I looked at.  One thing I did find is that there seems to be no "sacred concentration" and the ideal varies a lot between species. 

The article you posted seems to have been published in a journal on fruit growing. Since fruit seeds generally have very tough seed coats, such high concentrations might be necessary to penetrate and affect seedlings.  Things with softer coats might need less.  (Just a speculation) Although, now I kind of regret not doing a fourth option with 1000 PPM.

Some of the articles I looked at:
http://www.actahort.org/members/showpdf?booknrarnr=419_17
http://www.cababstractsplus.org/abstracts/Abstract.aspx?AcNo=20043117862
http://www.ingentaconnect.com/content/ista/sst/2009/00000037/00000002/art00019?crawler=true

Anyway, later on today I'll make a new thread for the experiment itself.


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The Plant Mage Guild

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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: crazyboy25]
    #10559110 -

crazyboy25 said:
Dr. uarewotueat do you have a reference for that post or how much was used?


i don't know what ratios he used with the BAP/GA3 mix, i don't think he knew either :lol:


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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: Dr. uarewotueat]
    #10562414 -

Dr. uarewotueat said:
crazyboy25 said:
Dr. uarewotueat do you have a reference for that post or how much was used?


i don't know what ratios he used with the BAP/GA3 mix, i don't think he knew either :lol:



Damn, well I recently purchased some MS media as well as BAP, KINETIN, IBA,and NAA along with the GA3 I already have I should have some experiments coming up soon.


I hope to do some stimulation of pupping with BAP as well as cactus tissue culture and I will share all the proportions and procedures, so look out.


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"Life without freedom is not life... freedom is life."

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Re: My GA3 came in today. Bring your experiment suggestions here. [Re: crazyboy25]
    #10565644 -

the only thing i know about the application of BAP is using it to stimulate pupping/branching in cacti.
not sure if that is your intention... but anyway, you need to make a paste with lanolin, approx 10% w/w and smear it on the aeroles.
this works, use a toothpick and scratch the aerole while you apply the paste.

i plan to do this to some of my rarer cacti shortly to produce some small pups for grafting :yesnod:


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Mushrooms, Mycology and Psychedelics > The Ethnobotanical Garden
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