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OfflineShadowTH
Dilated Mind

Registered: 08/13/01
Posts: 148
Loc: Wonderland
Last seen: 11 years, 8 months
Contamination setbacks..
    #683526 - 06/17/02 05:48 PM (20 years, 3 months ago)

Well, I finally got what I thought was a clean culture going from some half-assed prints I took a year ago. I've been using honey water, and adding h202 every now and then to keep it what I assumed to be clean and isolated from contams. But the jars I innoculated ended up with contams growing right along with the mycelium, and it's really knocked my confidence down.
Every time I think I'm getting somewhere, I end up back at square one. I guess this's one of those reminders of how much of a threat contams really are.
Anyway, Is there any advice on what I can do to get some clean healthy mycelium going again? I was thinking of adding a diluted bleach solution to the culture, but I'm wondering if it's even worth my time. Is it even possible to revive it? Thanks in advance.


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Offlinepowderfinger
member
Registered: 06/02/02
Posts: 112
Last seen: 19 years, 18 days
Re: Contamination setbacks.. [Re: ShadowTH]
    #684554 - 06/18/02 01:57 AM (20 years, 3 months ago)

I don't think you can save it but you can avoid contams in hte future by, of course, working more methodicly and in a clean enviroment. I doubt this is what you are looking for though.


--------------------
Shelter me from the powder and the finger,
cover me with the thought that pulled the trigger.


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OfflineShadowTH
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Registered: 08/13/01
Posts: 148
Loc: Wonderland
Last seen: 11 years, 8 months
Re: Contamination setbacks.. [Re: powderfinger]
    #684646 - 06/18/02 02:42 AM (20 years, 3 months ago)

*Sighs*
It's sad, because I have a small jar full of very rhizomorphic mycelium right now. I can't comprehend having to throw it out, but maybe it's for the best. Definately a lesson in not cutting corners..


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OfflineShroomNewb
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Registered: 11/09/01
Posts: 230
Last seen: 16 years, 8 months
Re: Contamination setbacks.. [Re: ShadowTH]
    #684842 - 06/18/02 04:50 AM (20 years, 3 months ago)

Personally I wouldn't mess with honey water.. but that's may just be me.
Right now I am doing grain to grain transfers.. it's easy.. and it works like a charm.


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OfflineShadowTH
Dilated Mind

Registered: 08/13/01
Posts: 148
Loc: Wonderland
Last seen: 11 years, 8 months
Re: Contamination setbacks.. [Re: ShroomNewb]
    #684972 - 06/18/02 07:48 AM (20 years, 3 months ago)

Yeah, I'm looking forward to getting into that. I don't mind the PF tek, but it's getting a bit old. However I don't have a pressure cooker at the moment, so I'm doing what I can without one. (which isn't much )


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OfflineDERRAYLD
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Registered: 05/14/02
Posts: 6,116
Loc: South Africa
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Re: Contamination setbacks.. [Re: ShadowTH]
    #684986 - 06/18/02 08:12 AM (20 years, 3 months ago)

nO way, the honey tech or any similar malt extract works much better inmo that multigrain.
Firstly I get growth after just 1 day after innoculation.
And it strong growth.
Think about it,
a) with multigrain you are exposing the grain to the air, in a glovebox no problems.
But then the same applies for honey water.
b) the grain your using might have been contaminated but being covered in contam and beeing beeten by the mycelium you`ll never know it.
So you do your grain transfer and wham, contamination.

I`ve never had problems with honey water, using a clone or just spores.
Maybe you just need to spray your work area or use a glovebox



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OfflineShadowTH
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Registered: 08/13/01
Posts: 148
Loc: Wonderland
Last seen: 11 years, 8 months
Re: Contamination setbacks.. [Re: DERRAYLD]
    #685565 - 06/18/02 04:39 PM (20 years, 3 months ago)

Well, I added a squirt of 1/9 bleach solution to the honey water last night, and plan to make at least one more attempt. This time around I'm definately going to be more sterile. I'm thinking maybe I didn't sterilize the syringe/needle enough, because the mycelium I've got growing in the jar looks extremely healthy & vigorous.
Really wish I had a pc right now.. I could be working with agar and stuff.
Anyway, any more suggestions are welcome.


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InvisibleHippie3
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Registered: 11/07/99
Posts: 3,090
Loc: mycotopia.net
Re: Contamination setbacks.. [Re: ShadowTH]
    #687317 - 06/19/02 11:15 AM (20 years, 3 months ago)

i'd be very interested to hear how your experiment with bleach turns out, could you either email me [hippie3 at hushmail.com] or pm me here when you find out, please ?


--------------------
Admin @ mycotopia.net
Mycotopia


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OfflineDERRAYLD
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Re: Contamination setbacks.. [Re: Hippie3]
    #687362 - 06/19/02 12:04 PM (20 years, 3 months ago)

works well hippie but not necessarily needed.
I only use a bit of bleach to help prolongue the life of the jar.
So far I`ve kept a jar for 4 weeks and it`s still transferrable.
and that`s without h202


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OfflineDERRAYLD
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Registered: 05/14/02
Posts: 6,116
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Re: Contamination setbacks.. [Re: DERRAYLD]
    #687366 - 06/19/02 12:06 PM (20 years, 3 months ago)

Next comes the horlicks and honey tech
Better than milo


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OfflineShadowTH
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Registered: 08/13/01
Posts: 148
Loc: Wonderland
Last seen: 11 years, 8 months
Re: Contamination setbacks.. [Re: Hippie3]
    #687975 - 06/19/02 05:51 PM (20 years, 3 months ago)

Of course, np.
Would definately be great when you can't use agar to isolate a dirty print. Probably hopeful thinking, but hey, no one ever got anywhere sticking to one proven method..


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